Job ID = 11245100 sra ファイルのダウンロード中... Completed: 280390K bytes transferred in 5 seconds (389969K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 16380529 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4590917/SRR7734558.sra Written 16380529 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4590917/SRR7734558.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 16380529 reads; of these: 16380529 (100.00%) were unpaired; of these: 1852957 (11.31%) aligned 0 times 12808327 (78.19%) aligned exactly 1 time 1719245 (10.50%) aligned >1 times 88.69% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6677559 / 14527572 = 0.4596 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 23:28:36: # Command line: callpeak -t SRX4590917.bam -f BAM -g 12100000 -n SRX4590917.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4590917.20 # format = BAM # ChIP-seq file = ['SRX4590917.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:28:36: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:28:36: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:28:36: # Command line: callpeak -t SRX4590917.bam -f BAM -g 12100000 -n SRX4590917.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4590917.05 # format = BAM # ChIP-seq file = ['SRX4590917.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:28:36: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:28:36: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:28:36: # Command line: callpeak -t SRX4590917.bam -f BAM -g 12100000 -n SRX4590917.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4590917.10 # format = BAM # ChIP-seq file = ['SRX4590917.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:28:36: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:28:36: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:28:43: 1000000 INFO @ Tue, 09 Oct 2018 23:28:43: 1000000 INFO @ Tue, 09 Oct 2018 23:28:43: 1000000 INFO @ Tue, 09 Oct 2018 23:28:50: 2000000 INFO @ Tue, 09 Oct 2018 23:28:50: 2000000 INFO @ Tue, 09 Oct 2018 23:28:50: 2000000 INFO @ Tue, 09 Oct 2018 23:28:57: 3000000 INFO @ Tue, 09 Oct 2018 23:28:57: 3000000 INFO @ Tue, 09 Oct 2018 23:28:57: 3000000 INFO @ Tue, 09 Oct 2018 23:29:03: 4000000 INFO @ Tue, 09 Oct 2018 23:29:04: 4000000 INFO @ Tue, 09 Oct 2018 23:29:04: 4000000 INFO @ Tue, 09 Oct 2018 23:29:11: 5000000 INFO @ Tue, 09 Oct 2018 23:29:11: 5000000 INFO @ Tue, 09 Oct 2018 23:29:11: 5000000 INFO @ Tue, 09 Oct 2018 23:29:17: 6000000 INFO @ Tue, 09 Oct 2018 23:29:18: 6000000 INFO @ Tue, 09 Oct 2018 23:29:18: 6000000 INFO @ Tue, 09 Oct 2018 23:29:24: 7000000 INFO @ Tue, 09 Oct 2018 23:29:25: 7000000 INFO @ Tue, 09 Oct 2018 23:29:25: 7000000 INFO @ Tue, 09 Oct 2018 23:29:30: #1 tag size is determined as 51 bps INFO @ Tue, 09 Oct 2018 23:29:30: #1 tag size = 51 INFO @ Tue, 09 Oct 2018 23:29:30: #1 total tags in treatment: 7850013 INFO @ Tue, 09 Oct 2018 23:29:30: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:29:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:29:30: #1 tags after filtering in treatment: 7850013 INFO @ Tue, 09 Oct 2018 23:29:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 09 Oct 2018 23:29:30: #1 finished! INFO @ Tue, 09 Oct 2018 23:29:30: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:29:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:29:30: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:29:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:29:30: Process for pairing-model is terminated! INFO @ Tue, 09 Oct 2018 23:29:30: #1 tag size is determined as 51 bps INFO @ Tue, 09 Oct 2018 23:29:30: #1 tag size = 51 INFO @ Tue, 09 Oct 2018 23:29:30: #1 total tags in treatment: 7850013 INFO @ Tue, 09 Oct 2018 23:29:30: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:29:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cat: SRX4590917.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4590917.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4590917.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4590917.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:29:30: #1 tags after filtering in treatment: 7850013 INFO @ Tue, 09 Oct 2018 23:29:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 09 Oct 2018 23:29:30: #1 finished! INFO @ Tue, 09 Oct 2018 23:29:30: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:29:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:29:31: #1 tag size is determined as 51 bps INFO @ Tue, 09 Oct 2018 23:29:31: #1 tag size = 51 INFO @ Tue, 09 Oct 2018 23:29:31: #1 total tags in treatment: 7850013 INFO @ Tue, 09 Oct 2018 23:29:31: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:29:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:29:31: #1 tags after filtering in treatment: 7850013 INFO @ Tue, 09 Oct 2018 23:29:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 09 Oct 2018 23:29:31: #1 finished! INFO @ Tue, 09 Oct 2018 23:29:31: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:29:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:29:31: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:29:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:29:31: Process for pairing-model is terminated! cat: SRX4590917.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4590917.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4590917.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4590917.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:29:31: #2 number of paired peaks: 0 WARNING @ Tue, 09 Oct 2018 23:29:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 09 Oct 2018 23:29:31: Process for pairing-model is terminated! cat: SRX4590917.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4590917.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4590917.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4590917.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。