Job ID = 12531688 SRX = SRX4555057 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18339536 spots for SRR7696758/SRR7696758.sra Written 18339536 spots for SRR7696758/SRR7696758.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:22:09 18339536 reads; of these: 18339536 (100.00%) were paired; of these: 1082290 (5.90%) aligned concordantly 0 times 10605306 (57.83%) aligned concordantly exactly 1 time 6651940 (36.27%) aligned concordantly >1 times ---- 1082290 pairs aligned concordantly 0 times; of these: 68086 (6.29%) aligned discordantly 1 time ---- 1014204 pairs aligned 0 times concordantly or discordantly; of these: 2028408 mates make up the pairs; of these: 1654472 (81.57%) aligned 0 times 215077 (10.60%) aligned exactly 1 time 158859 (7.83%) aligned >1 times 95.49% overall alignment rate Time searching: 00:22:09 Overall time: 00:22:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2480343 / 7428022 = 0.3339 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:44:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:44:57: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:44:57: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:45:05: 1000000 INFO @ Sat, 17 Apr 2021 09:45:12: 2000000 INFO @ Sat, 17 Apr 2021 09:45:19: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:45:27: 4000000 INFO @ Sat, 17 Apr 2021 09:45:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:45:27: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:45:27: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:45:35: 5000000 INFO @ Sat, 17 Apr 2021 09:45:36: 1000000 INFO @ Sat, 17 Apr 2021 09:45:43: 6000000 INFO @ Sat, 17 Apr 2021 09:45:44: 2000000 INFO @ Sat, 17 Apr 2021 09:45:51: 7000000 INFO @ Sat, 17 Apr 2021 09:45:52: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:45:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:45:57: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:45:57: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:45:59: 8000000 INFO @ Sat, 17 Apr 2021 09:46:01: 4000000 INFO @ Sat, 17 Apr 2021 09:46:05: 1000000 INFO @ Sat, 17 Apr 2021 09:46:07: 9000000 INFO @ Sat, 17 Apr 2021 09:46:09: 5000000 INFO @ Sat, 17 Apr 2021 09:46:13: 2000000 INFO @ Sat, 17 Apr 2021 09:46:16: 10000000 INFO @ Sat, 17 Apr 2021 09:46:17: 6000000 INFO @ Sat, 17 Apr 2021 09:46:22: 3000000 INFO @ Sat, 17 Apr 2021 09:46:24: 11000000 INFO @ Sat, 17 Apr 2021 09:46:25: 7000000 INFO @ Sat, 17 Apr 2021 09:46:30: 4000000 INFO @ Sat, 17 Apr 2021 09:46:33: 12000000 INFO @ Sat, 17 Apr 2021 09:46:33: 8000000 INFO @ Sat, 17 Apr 2021 09:46:38: 5000000 INFO @ Sat, 17 Apr 2021 09:46:41: 9000000 INFO @ Sat, 17 Apr 2021 09:46:41: 13000000 INFO @ Sat, 17 Apr 2021 09:46:46: 6000000 INFO @ Sat, 17 Apr 2021 09:46:49: 10000000 INFO @ Sat, 17 Apr 2021 09:46:50: 14000000 INFO @ Sat, 17 Apr 2021 09:46:54: 7000000 INFO @ Sat, 17 Apr 2021 09:46:57: 15000000 INFO @ Sat, 17 Apr 2021 09:46:58: 11000000 INFO @ Sat, 17 Apr 2021 09:47:02: 8000000 INFO @ Sat, 17 Apr 2021 09:47:05: 16000000 INFO @ Sat, 17 Apr 2021 09:47:06: 12000000 INFO @ Sat, 17 Apr 2021 09:47:11: 9000000 INFO @ Sat, 17 Apr 2021 09:47:13: 17000000 INFO @ Sat, 17 Apr 2021 09:47:14: 13000000 INFO @ Sat, 17 Apr 2021 09:47:19: 10000000 INFO @ Sat, 17 Apr 2021 09:47:21: 18000000 INFO @ Sat, 17 Apr 2021 09:47:22: 14000000 INFO @ Sat, 17 Apr 2021 09:47:27: 11000000 INFO @ Sat, 17 Apr 2021 09:47:29: 19000000 INFO @ Sat, 17 Apr 2021 09:47:30: 15000000 INFO @ Sat, 17 Apr 2021 09:47:36: 12000000 INFO @ Sat, 17 Apr 2021 09:47:36: 20000000 INFO @ Sat, 17 Apr 2021 09:47:38: 16000000 INFO @ Sat, 17 Apr 2021 09:47:44: 13000000 INFO @ Sat, 17 Apr 2021 09:47:44: 21000000 INFO @ Sat, 17 Apr 2021 09:47:45: 17000000 INFO @ Sat, 17 Apr 2021 09:47:52: 22000000 INFO @ Sat, 17 Apr 2021 09:47:52: 14000000 INFO @ Sat, 17 Apr 2021 09:47:53: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 09:48:00: 15000000 INFO @ Sat, 17 Apr 2021 09:48:00: 23000000 INFO @ Sat, 17 Apr 2021 09:48:01: 19000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 09:48:07: 16000000 INFO @ Sat, 17 Apr 2021 09:48:08: 20000000 INFO @ Sat, 17 Apr 2021 09:48:09: 24000000 INFO @ Sat, 17 Apr 2021 09:48:15: 17000000 INFO @ Sat, 17 Apr 2021 09:48:16: 21000000 INFO @ Sat, 17 Apr 2021 09:48:17: 25000000 INFO @ Sat, 17 Apr 2021 09:48:23: 18000000 INFO @ Sat, 17 Apr 2021 09:48:23: 22000000 INFO @ Sat, 17 Apr 2021 09:48:26: 26000000 INFO @ Sat, 17 Apr 2021 09:48:30: 19000000 INFO @ Sat, 17 Apr 2021 09:48:31: 23000000 INFO @ Sat, 17 Apr 2021 09:48:35: 27000000 INFO @ Sat, 17 Apr 2021 09:48:38: 20000000 INFO @ Sat, 17 Apr 2021 09:48:40: 24000000 INFO @ Sat, 17 Apr 2021 09:48:43: 28000000 INFO @ Sat, 17 Apr 2021 09:48:45: 21000000 INFO @ Sat, 17 Apr 2021 09:48:48: 25000000 INFO @ Sat, 17 Apr 2021 09:48:52: 29000000 INFO @ Sat, 17 Apr 2021 09:48:53: 22000000 INFO @ Sat, 17 Apr 2021 09:48:56: 26000000 INFO @ Sat, 17 Apr 2021 09:49:00: 30000000 INFO @ Sat, 17 Apr 2021 09:49:01: 23000000 INFO @ Sat, 17 Apr 2021 09:49:01: #1 tag size is determined as 83 bps INFO @ Sat, 17 Apr 2021 09:49:01: #1 tag size = 83 INFO @ Sat, 17 Apr 2021 09:49:01: #1 total tags in treatment: 14781642 INFO @ Sat, 17 Apr 2021 09:49:01: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:49:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:49:01: #1 tags after filtering in treatment: 3882840 INFO @ Sat, 17 Apr 2021 09:49:01: #1 Redundant rate of treatment: 0.74 INFO @ Sat, 17 Apr 2021 09:49:01: #1 finished! INFO @ Sat, 17 Apr 2021 09:49:01: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:49:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:49:02: #2 number of paired peaks: 25 WARNING @ Sat, 17 Apr 2021 09:49:02: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:49:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:49:05: 27000000 INFO @ Sat, 17 Apr 2021 09:49:09: 24000000 INFO @ Sat, 17 Apr 2021 09:49:13: 28000000 INFO @ Sat, 17 Apr 2021 09:49:17: 25000000 INFO @ Sat, 17 Apr 2021 09:49:21: 29000000 INFO @ Sat, 17 Apr 2021 09:49:25: 26000000 INFO @ Sat, 17 Apr 2021 09:49:30: 30000000 INFO @ Sat, 17 Apr 2021 09:49:30: #1 tag size is determined as 83 bps INFO @ Sat, 17 Apr 2021 09:49:30: #1 tag size = 83 INFO @ Sat, 17 Apr 2021 09:49:30: #1 total tags in treatment: 14781642 INFO @ Sat, 17 Apr 2021 09:49:30: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:49:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:49:31: #1 tags after filtering in treatment: 3882840 INFO @ Sat, 17 Apr 2021 09:49:31: #1 Redundant rate of treatment: 0.74 INFO @ Sat, 17 Apr 2021 09:49:31: #1 finished! INFO @ Sat, 17 Apr 2021 09:49:31: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:49:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:49:31: #2 number of paired peaks: 25 WARNING @ Sat, 17 Apr 2021 09:49:31: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:49:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:49:33: 27000000 INFO @ Sat, 17 Apr 2021 09:49:41: 28000000 INFO @ Sat, 17 Apr 2021 09:49:48: 29000000 INFO @ Sat, 17 Apr 2021 09:49:56: 30000000 INFO @ Sat, 17 Apr 2021 09:49:57: #1 tag size is determined as 83 bps INFO @ Sat, 17 Apr 2021 09:49:57: #1 tag size = 83 INFO @ Sat, 17 Apr 2021 09:49:57: #1 total tags in treatment: 14781642 INFO @ Sat, 17 Apr 2021 09:49:57: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:49:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:49:57: #1 tags after filtering in treatment: 3882840 INFO @ Sat, 17 Apr 2021 09:49:57: #1 Redundant rate of treatment: 0.74 INFO @ Sat, 17 Apr 2021 09:49:57: #1 finished! INFO @ Sat, 17 Apr 2021 09:49:57: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:49:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:49:57: #2 number of paired peaks: 25 WARNING @ Sat, 17 Apr 2021 09:49:57: Too few paired peaks (25) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:49:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555057/SRX4555057.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling