Job ID = 12531685 SRX = SRX4555054 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11416059 spots for SRR7696755/SRR7696755.sra Written 11416059 spots for SRR7696755/SRR7696755.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:02 11416059 reads; of these: 11416059 (100.00%) were paired; of these: 613602 (5.37%) aligned concordantly 0 times 6703301 (58.72%) aligned concordantly exactly 1 time 4099156 (35.91%) aligned concordantly >1 times ---- 613602 pairs aligned concordantly 0 times; of these: 44596 (7.27%) aligned discordantly 1 time ---- 569006 pairs aligned 0 times concordantly or discordantly; of these: 1138012 mates make up the pairs; of these: 899458 (79.04%) aligned 0 times 142031 (12.48%) aligned exactly 1 time 96523 (8.48%) aligned >1 times 96.06% overall alignment rate Time searching: 00:25:02 Overall time: 00:25:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1642672 / 5319279 = 0.3088 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:45:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:45:52: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:45:52: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:46:11: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:46:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:46:22: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:46:22: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:46:27: 2000000 INFO @ Sat, 17 Apr 2021 09:46:40: 1000000 INFO @ Sat, 17 Apr 2021 09:46:46: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:46:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:46:53: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:46:53: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:47:04: 2000000 INFO @ Sat, 17 Apr 2021 09:47:11: 4000000 INFO @ Sat, 17 Apr 2021 09:47:24: 1000000 INFO @ Sat, 17 Apr 2021 09:47:38: 5000000 INFO @ Sat, 17 Apr 2021 09:47:39: 3000000 INFO @ Sat, 17 Apr 2021 09:48:02: 2000000 INFO @ Sat, 17 Apr 2021 09:48:08: 6000000 INFO @ Sat, 17 Apr 2021 09:48:12: 4000000 BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 09:48:38: 7000000 INFO @ Sat, 17 Apr 2021 09:48:39: 5000000 INFO @ Sat, 17 Apr 2021 09:48:40: 3000000 INFO @ Sat, 17 Apr 2021 09:48:59: 8000000 INFO @ Sat, 17 Apr 2021 09:49:04: 6000000 INFO @ Sat, 17 Apr 2021 09:49:06: 4000000 INFO @ Sat, 17 Apr 2021 09:49:25: 5000000 INFO @ Sat, 17 Apr 2021 09:49:27: 9000000 INFO @ Sat, 17 Apr 2021 09:49:32: 7000000 INFO @ Sat, 17 Apr 2021 09:49:49: 6000000 INFO @ Sat, 17 Apr 2021 09:49:49: 10000000 INFO @ Sat, 17 Apr 2021 09:49:55: 8000000 INFO @ Sat, 17 Apr 2021 09:50:08: 11000000 INFO @ Sat, 17 Apr 2021 09:50:09: 7000000 INFO @ Sat, 17 Apr 2021 09:50:16: 9000000 INFO @ Sat, 17 Apr 2021 09:50:28: 8000000 INFO @ Sat, 17 Apr 2021 09:50:33: 12000000 INFO @ Sat, 17 Apr 2021 09:50:41: 10000000 INFO @ Sat, 17 Apr 2021 09:50:47: 9000000 INFO @ Sat, 17 Apr 2021 09:50:57: 13000000 INFO @ Sat, 17 Apr 2021 09:51:03: 11000000 INFO @ Sat, 17 Apr 2021 09:51:05: 10000000 INFO @ Sat, 17 Apr 2021 09:51:24: 11000000 INFO @ Sat, 17 Apr 2021 09:51:25: 12000000 INFO @ Sat, 17 Apr 2021 09:51:25: 14000000 INFO @ Sat, 17 Apr 2021 09:51:42: 12000000 INFO @ Sat, 17 Apr 2021 09:51:46: 13000000 INFO @ Sat, 17 Apr 2021 09:51:49: 15000000 INFO @ Sat, 17 Apr 2021 09:52:02: 13000000 INFO @ Sat, 17 Apr 2021 09:52:11: 14000000 INFO @ Sat, 17 Apr 2021 09:52:12: 16000000 INFO @ Sat, 17 Apr 2021 09:52:27: 14000000 INFO @ Sat, 17 Apr 2021 09:52:46: 15000000 INFO @ Sat, 17 Apr 2021 09:52:57: 17000000 INFO @ Sat, 17 Apr 2021 09:53:12: 15000000 INFO @ Sat, 17 Apr 2021 09:53:18: 16000000 INFO @ Sat, 17 Apr 2021 09:53:54: 17000000 INFO @ Sat, 17 Apr 2021 09:53:55: 18000000 INFO @ Sat, 17 Apr 2021 09:54:01: 16000000 INFO @ Sat, 17 Apr 2021 09:54:28: 18000000 INFO @ Sat, 17 Apr 2021 09:54:33: #1 tag size is determined as 76 bps INFO @ Sat, 17 Apr 2021 09:54:33: #1 tag size = 76 INFO @ Sat, 17 Apr 2021 09:54:33: #1 total tags in treatment: 9162170 INFO @ Sat, 17 Apr 2021 09:54:33: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:54:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:54:33: #1 tags after filtering in treatment: 2784016 INFO @ Sat, 17 Apr 2021 09:54:33: #1 Redundant rate of treatment: 0.70 INFO @ Sat, 17 Apr 2021 09:54:33: #1 finished! INFO @ Sat, 17 Apr 2021 09:54:33: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:54:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:54:34: #2 number of paired peaks: 49 WARNING @ Sat, 17 Apr 2021 09:54:34: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:54:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:54:54: #1 tag size is determined as 76 bps INFO @ Sat, 17 Apr 2021 09:54:54: #1 tag size = 76 INFO @ Sat, 17 Apr 2021 09:54:54: #1 total tags in treatment: 9162170 INFO @ Sat, 17 Apr 2021 09:54:54: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:54:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:54:54: #1 tags after filtering in treatment: 2784016 INFO @ Sat, 17 Apr 2021 09:54:54: #1 Redundant rate of treatment: 0.70 INFO @ Sat, 17 Apr 2021 09:54:54: #1 finished! INFO @ Sat, 17 Apr 2021 09:54:54: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:54:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:54:55: #2 number of paired peaks: 49 WARNING @ Sat, 17 Apr 2021 09:54:55: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:54:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:54:59: 17000000 INFO @ Sat, 17 Apr 2021 09:55:36: 18000000 INFO @ Sat, 17 Apr 2021 09:55:53: #1 tag size is determined as 76 bps INFO @ Sat, 17 Apr 2021 09:55:53: #1 tag size = 76 INFO @ Sat, 17 Apr 2021 09:55:53: #1 total tags in treatment: 9162170 INFO @ Sat, 17 Apr 2021 09:55:53: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:55:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:55:53: #1 tags after filtering in treatment: 2784016 INFO @ Sat, 17 Apr 2021 09:55:53: #1 Redundant rate of treatment: 0.70 INFO @ Sat, 17 Apr 2021 09:55:53: #1 finished! INFO @ Sat, 17 Apr 2021 09:55:53: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:55:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:55:53: #2 number of paired peaks: 49 WARNING @ Sat, 17 Apr 2021 09:55:53: Too few paired peaks (49) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:55:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 13 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555054/SRX4555054.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling