Job ID = 12531670 SRX = SRX4555042 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18126426 spots for SRR7696739/SRR7696739.sra Written 18126426 spots for SRR7696739/SRR7696739.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:20:35 18126426 reads; of these: 18126426 (100.00%) were paired; of these: 917831 (5.06%) aligned concordantly 0 times 9812091 (54.13%) aligned concordantly exactly 1 time 7396504 (40.81%) aligned concordantly >1 times ---- 917831 pairs aligned concordantly 0 times; of these: 69443 (7.57%) aligned discordantly 1 time ---- 848388 pairs aligned 0 times concordantly or discordantly; of these: 1696776 mates make up the pairs; of these: 1325357 (78.11%) aligned 0 times 203070 (11.97%) aligned exactly 1 time 168349 (9.92%) aligned >1 times 96.34% overall alignment rate Time searching: 00:20:35 Overall time: 00:20:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3012317 / 8110601 = 0.3714 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:34:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:34:14: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:34:14: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:34:22: 1000000 INFO @ Sat, 17 Apr 2021 09:34:30: 2000000 INFO @ Sat, 17 Apr 2021 09:34:38: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:34:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:34:44: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:34:44: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:34:45: 4000000 INFO @ Sat, 17 Apr 2021 09:34:55: 1000000 INFO @ Sat, 17 Apr 2021 09:34:55: 5000000 INFO @ Sat, 17 Apr 2021 09:35:05: 6000000 INFO @ Sat, 17 Apr 2021 09:35:05: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:35:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:35:14: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:35:14: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:35:15: 7000000 INFO @ Sat, 17 Apr 2021 09:35:15: 3000000 INFO @ Sat, 17 Apr 2021 09:35:25: 8000000 INFO @ Sat, 17 Apr 2021 09:35:25: 1000000 INFO @ Sat, 17 Apr 2021 09:35:26: 4000000 INFO @ Sat, 17 Apr 2021 09:35:35: 9000000 INFO @ Sat, 17 Apr 2021 09:35:36: 2000000 INFO @ Sat, 17 Apr 2021 09:35:37: 5000000 INFO @ Sat, 17 Apr 2021 09:35:45: 10000000 INFO @ Sat, 17 Apr 2021 09:35:47: 3000000 INFO @ Sat, 17 Apr 2021 09:35:47: 6000000 INFO @ Sat, 17 Apr 2021 09:35:55: 11000000 INFO @ Sat, 17 Apr 2021 09:35:57: 4000000 INFO @ Sat, 17 Apr 2021 09:35:58: 7000000 INFO @ Sat, 17 Apr 2021 09:36:05: 12000000 INFO @ Sat, 17 Apr 2021 09:36:07: 5000000 INFO @ Sat, 17 Apr 2021 09:36:08: 8000000 INFO @ Sat, 17 Apr 2021 09:36:15: 13000000 INFO @ Sat, 17 Apr 2021 09:36:18: 6000000 INFO @ Sat, 17 Apr 2021 09:36:18: 9000000 INFO @ Sat, 17 Apr 2021 09:36:24: 14000000 INFO @ Sat, 17 Apr 2021 09:36:29: 7000000 INFO @ Sat, 17 Apr 2021 09:36:29: 10000000 INFO @ Sat, 17 Apr 2021 09:36:33: 15000000 INFO @ Sat, 17 Apr 2021 09:36:39: 8000000 INFO @ Sat, 17 Apr 2021 09:36:40: 11000000 INFO @ Sat, 17 Apr 2021 09:36:42: 16000000 INFO @ Sat, 17 Apr 2021 09:36:50: 9000000 INFO @ Sat, 17 Apr 2021 09:36:51: 12000000 INFO @ Sat, 17 Apr 2021 09:36:52: 17000000 INFO @ Sat, 17 Apr 2021 09:37:01: 10000000 INFO @ Sat, 17 Apr 2021 09:37:01: 18000000 INFO @ Sat, 17 Apr 2021 09:37:01: 13000000 INFO @ Sat, 17 Apr 2021 09:37:10: 19000000 INFO @ Sat, 17 Apr 2021 09:37:11: 14000000 INFO @ Sat, 17 Apr 2021 09:37:12: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 09:37:20: 20000000 INFO @ Sat, 17 Apr 2021 09:37:21: 15000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 09:37:22: 12000000 INFO @ Sat, 17 Apr 2021 09:37:29: 21000000 INFO @ Sat, 17 Apr 2021 09:37:31: 16000000 INFO @ Sat, 17 Apr 2021 09:37:33: 13000000 INFO @ Sat, 17 Apr 2021 09:37:39: 22000000 INFO @ Sat, 17 Apr 2021 09:37:41: 17000000 INFO @ Sat, 17 Apr 2021 09:37:43: 14000000 INFO @ Sat, 17 Apr 2021 09:37:49: 23000000 INFO @ Sat, 17 Apr 2021 09:37:50: 18000000 INFO @ Sat, 17 Apr 2021 09:37:53: 15000000 INFO @ Sat, 17 Apr 2021 09:37:59: 24000000 INFO @ Sat, 17 Apr 2021 09:38:00: 19000000 INFO @ Sat, 17 Apr 2021 09:38:02: 16000000 INFO @ Sat, 17 Apr 2021 09:38:09: 25000000 INFO @ Sat, 17 Apr 2021 09:38:10: 20000000 INFO @ Sat, 17 Apr 2021 09:38:13: 17000000 INFO @ Sat, 17 Apr 2021 09:38:20: 26000000 INFO @ Sat, 17 Apr 2021 09:38:20: 21000000 INFO @ Sat, 17 Apr 2021 09:38:23: 18000000 INFO @ Sat, 17 Apr 2021 09:38:30: 27000000 INFO @ Sat, 17 Apr 2021 09:38:30: 22000000 INFO @ Sat, 17 Apr 2021 09:38:33: 19000000 INFO @ Sat, 17 Apr 2021 09:38:40: 28000000 INFO @ Sat, 17 Apr 2021 09:38:40: 23000000 INFO @ Sat, 17 Apr 2021 09:38:43: 20000000 INFO @ Sat, 17 Apr 2021 09:38:49: #1 tag size is determined as 81 bps INFO @ Sat, 17 Apr 2021 09:38:49: #1 tag size = 81 INFO @ Sat, 17 Apr 2021 09:38:49: #1 total tags in treatment: 14199983 INFO @ Sat, 17 Apr 2021 09:38:49: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:38:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:38:49: #1 tags after filtering in treatment: 3778387 INFO @ Sat, 17 Apr 2021 09:38:49: #1 Redundant rate of treatment: 0.73 INFO @ Sat, 17 Apr 2021 09:38:49: #1 finished! INFO @ Sat, 17 Apr 2021 09:38:49: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:38:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:38:50: #2 number of paired peaks: 29 WARNING @ Sat, 17 Apr 2021 09:38:50: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:38:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:38:51: 24000000 INFO @ Sat, 17 Apr 2021 09:38:53: 21000000 INFO @ Sat, 17 Apr 2021 09:39:02: 25000000 INFO @ Sat, 17 Apr 2021 09:39:03: 22000000 INFO @ Sat, 17 Apr 2021 09:39:12: 26000000 INFO @ Sat, 17 Apr 2021 09:39:14: 23000000 INFO @ Sat, 17 Apr 2021 09:39:22: 27000000 INFO @ Sat, 17 Apr 2021 09:39:24: 24000000 INFO @ Sat, 17 Apr 2021 09:39:33: 28000000 INFO @ Sat, 17 Apr 2021 09:39:35: 25000000 INFO @ Sat, 17 Apr 2021 09:39:42: #1 tag size is determined as 81 bps INFO @ Sat, 17 Apr 2021 09:39:42: #1 tag size = 81 INFO @ Sat, 17 Apr 2021 09:39:42: #1 total tags in treatment: 14199983 INFO @ Sat, 17 Apr 2021 09:39:42: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:39:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:39:42: #1 tags after filtering in treatment: 3778387 INFO @ Sat, 17 Apr 2021 09:39:42: #1 Redundant rate of treatment: 0.73 INFO @ Sat, 17 Apr 2021 09:39:42: #1 finished! INFO @ Sat, 17 Apr 2021 09:39:42: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:39:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:39:42: #2 number of paired peaks: 29 WARNING @ Sat, 17 Apr 2021 09:39:42: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:39:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:39:46: 26000000 INFO @ Sat, 17 Apr 2021 09:39:55: 27000000 INFO @ Sat, 17 Apr 2021 09:40:05: 28000000 INFO @ Sat, 17 Apr 2021 09:40:13: #1 tag size is determined as 81 bps INFO @ Sat, 17 Apr 2021 09:40:13: #1 tag size = 81 INFO @ Sat, 17 Apr 2021 09:40:13: #1 total tags in treatment: 14199983 INFO @ Sat, 17 Apr 2021 09:40:13: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:40:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:40:14: #1 tags after filtering in treatment: 3778387 INFO @ Sat, 17 Apr 2021 09:40:14: #1 Redundant rate of treatment: 0.73 INFO @ Sat, 17 Apr 2021 09:40:14: #1 finished! INFO @ Sat, 17 Apr 2021 09:40:14: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:40:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:40:14: #2 number of paired peaks: 29 WARNING @ Sat, 17 Apr 2021 09:40:14: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 09:40:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4555042/SRX4555042.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling