Job ID = 12531656 SRX = SRX4555030 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 3224976 spots for SRR7696725/SRR7696725.sra Written 3224976 spots for SRR7696725/SRR7696725.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:01 3224976 reads; of these: 3224976 (100.00%) were paired; of these: 182924 (5.67%) aligned concordantly 0 times 1734565 (53.79%) aligned concordantly exactly 1 time 1307487 (40.54%) aligned concordantly >1 times ---- 182924 pairs aligned concordantly 0 times; of these: 14376 (7.86%) aligned discordantly 1 time ---- 168548 pairs aligned 0 times concordantly or discordantly; of these: 337096 mates make up the pairs; of these: 272216 (80.75%) aligned 0 times 33956 (10.07%) aligned exactly 1 time 30924 (9.17%) aligned >1 times 95.78% overall alignment rate Time searching: 00:04:01 Overall time: 00:04:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 423495 / 1545894 = 0.2739 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:00:21: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:00:21: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:00:31: 1000000 INFO @ Sat, 17 Apr 2021 09:00:41: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:00:51: 3000000 INFO @ Sat, 17 Apr 2021 09:00:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:00:51: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:00:51: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:01:02: 1000000 INFO @ Sat, 17 Apr 2021 09:01:02: 4000000 INFO @ Sat, 17 Apr 2021 09:01:13: 2000000 INFO @ Sat, 17 Apr 2021 09:01:14: 5000000 INFO @ Sat, 17 Apr 2021 09:01:18: #1 tag size is determined as 120 bps INFO @ Sat, 17 Apr 2021 09:01:18: #1 tag size = 120 INFO @ Sat, 17 Apr 2021 09:01:18: #1 total tags in treatment: 2618832 INFO @ Sat, 17 Apr 2021 09:01:18: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:01:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:01:18: #1 tags after filtering in treatment: 1031734 INFO @ Sat, 17 Apr 2021 09:01:18: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 17 Apr 2021 09:01:18: #1 finished! INFO @ Sat, 17 Apr 2021 09:01:18: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:01:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:01:18: #2 number of paired peaks: 452 WARNING @ Sat, 17 Apr 2021 09:01:18: Fewer paired peaks (452) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 452 pairs to build model! INFO @ Sat, 17 Apr 2021 09:01:18: start model_add_line... INFO @ Sat, 17 Apr 2021 09:01:18: start X-correlation... INFO @ Sat, 17 Apr 2021 09:01:18: end of X-cor INFO @ Sat, 17 Apr 2021 09:01:18: #2 finished! INFO @ Sat, 17 Apr 2021 09:01:18: #2 predicted fragment length is 101 bps INFO @ Sat, 17 Apr 2021 09:01:18: #2 alternative fragment length(s) may be 101 bps INFO @ Sat, 17 Apr 2021 09:01:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.05_model.r WARNING @ Sat, 17 Apr 2021 09:01:18: #2 Since the d (101) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 17 Apr 2021 09:01:18: #2 You may need to consider one of the other alternative d(s): 101 WARNING @ Sat, 17 Apr 2021 09:01:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 17 Apr 2021 09:01:18: #3 Call peaks... INFO @ Sat, 17 Apr 2021 09:01:18: #3 Pre-compute pvalue-qvalue table... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 09:01:21: #3 Call peaks for each chromosome... INFO @ Sat, 17 Apr 2021 09:01:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 09:01:21: #1 read tag files... INFO @ Sat, 17 Apr 2021 09:01:21: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 09:01:22: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.05_peaks.xls INFO @ Sat, 17 Apr 2021 09:01:22: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.05_peaks.narrowPeak INFO @ Sat, 17 Apr 2021 09:01:22: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.05_summits.bed INFO @ Sat, 17 Apr 2021 09:01:22: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (1912 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:01:22: 3000000 INFO @ Sat, 17 Apr 2021 09:01:33: 4000000 INFO @ Sat, 17 Apr 2021 09:01:33: 1000000 INFO @ Sat, 17 Apr 2021 09:01:44: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 09:01:46: 2000000 INFO @ Sat, 17 Apr 2021 09:01:47: #1 tag size is determined as 120 bps INFO @ Sat, 17 Apr 2021 09:01:47: #1 tag size = 120 INFO @ Sat, 17 Apr 2021 09:01:47: #1 total tags in treatment: 2618832 INFO @ Sat, 17 Apr 2021 09:01:47: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:01:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:01:48: #1 tags after filtering in treatment: 1031734 INFO @ Sat, 17 Apr 2021 09:01:48: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 17 Apr 2021 09:01:48: #1 finished! INFO @ Sat, 17 Apr 2021 09:01:48: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:01:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:01:48: #2 number of paired peaks: 452 WARNING @ Sat, 17 Apr 2021 09:01:48: Fewer paired peaks (452) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 452 pairs to build model! INFO @ Sat, 17 Apr 2021 09:01:48: start model_add_line... INFO @ Sat, 17 Apr 2021 09:01:48: start X-correlation... INFO @ Sat, 17 Apr 2021 09:01:48: end of X-cor INFO @ Sat, 17 Apr 2021 09:01:48: #2 finished! INFO @ Sat, 17 Apr 2021 09:01:48: #2 predicted fragment length is 101 bps INFO @ Sat, 17 Apr 2021 09:01:48: #2 alternative fragment length(s) may be 101 bps INFO @ Sat, 17 Apr 2021 09:01:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.10_model.r WARNING @ Sat, 17 Apr 2021 09:01:48: #2 Since the d (101) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 17 Apr 2021 09:01:48: #2 You may need to consider one of the other alternative d(s): 101 WARNING @ Sat, 17 Apr 2021 09:01:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 17 Apr 2021 09:01:48: #3 Call peaks... INFO @ Sat, 17 Apr 2021 09:01:48: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 09:01:50: #3 Call peaks for each chromosome... INFO @ Sat, 17 Apr 2021 09:01:52: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.10_peaks.xls INFO @ Sat, 17 Apr 2021 09:01:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.10_peaks.narrowPeak INFO @ Sat, 17 Apr 2021 09:01:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.10_summits.bed INFO @ Sat, 17 Apr 2021 09:01:52: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (1321 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 09:01:56: 3000000 INFO @ Sat, 17 Apr 2021 09:02:07: 4000000 INFO @ Sat, 17 Apr 2021 09:02:17: 5000000 INFO @ Sat, 17 Apr 2021 09:02:21: #1 tag size is determined as 120 bps INFO @ Sat, 17 Apr 2021 09:02:21: #1 tag size = 120 INFO @ Sat, 17 Apr 2021 09:02:21: #1 total tags in treatment: 2618832 INFO @ Sat, 17 Apr 2021 09:02:21: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 09:02:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 09:02:21: #1 tags after filtering in treatment: 1031734 INFO @ Sat, 17 Apr 2021 09:02:21: #1 Redundant rate of treatment: 0.61 INFO @ Sat, 17 Apr 2021 09:02:21: #1 finished! INFO @ Sat, 17 Apr 2021 09:02:21: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 09:02:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 09:02:21: #2 number of paired peaks: 452 WARNING @ Sat, 17 Apr 2021 09:02:21: Fewer paired peaks (452) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 452 pairs to build model! INFO @ Sat, 17 Apr 2021 09:02:21: start model_add_line... INFO @ Sat, 17 Apr 2021 09:02:21: start X-correlation... INFO @ Sat, 17 Apr 2021 09:02:21: end of X-cor INFO @ Sat, 17 Apr 2021 09:02:21: #2 finished! INFO @ Sat, 17 Apr 2021 09:02:21: #2 predicted fragment length is 101 bps INFO @ Sat, 17 Apr 2021 09:02:21: #2 alternative fragment length(s) may be 101 bps INFO @ Sat, 17 Apr 2021 09:02:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.20_model.r WARNING @ Sat, 17 Apr 2021 09:02:21: #2 Since the d (101) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 17 Apr 2021 09:02:21: #2 You may need to consider one of the other alternative d(s): 101 WARNING @ Sat, 17 Apr 2021 09:02:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 17 Apr 2021 09:02:21: #3 Call peaks... INFO @ Sat, 17 Apr 2021 09:02:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 17 Apr 2021 09:02:24: #3 Call peaks for each chromosome... INFO @ Sat, 17 Apr 2021 09:02:25: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.20_peaks.xls INFO @ Sat, 17 Apr 2021 09:02:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.20_peaks.narrowPeak INFO @ Sat, 17 Apr 2021 09:02:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/SRX4555030/SRX4555030.20_summits.bed INFO @ Sat, 17 Apr 2021 09:02:25: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (754 records, 4 fields): 7 millis CompletedMACS2peakCalling