Job ID = 11244980 sra ファイルのダウンロード中... Completed: 240406K bytes transferred in 7 seconds (275202K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 7788222 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4554691/SRR7696364.sra Written 7788222 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4554691/SRR7696364.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:30 7788222 reads; of these: 7788222 (100.00%) were paired; of these: 6557747 (84.20%) aligned concordantly 0 times 901792 (11.58%) aligned concordantly exactly 1 time 328683 (4.22%) aligned concordantly >1 times ---- 6557747 pairs aligned concordantly 0 times; of these: 944 (0.01%) aligned discordantly 1 time ---- 6556803 pairs aligned 0 times concordantly or discordantly; of these: 13113606 mates make up the pairs; of these: 10878535 (82.96%) aligned 0 times 1679143 (12.80%) aligned exactly 1 time 555928 (4.24%) aligned >1 times 30.16% overall alignment rate Time searching: 00:02:30 Overall time: 00:02:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 565006 / 1230853 = 0.4590 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 23:01:37: # Command line: callpeak -t SRX4554691.bam -f BAM -g 12100000 -n SRX4554691.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4554691.10 # format = BAM # ChIP-seq file = ['SRX4554691.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:01:37: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:01:37: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:01:37: # Command line: callpeak -t SRX4554691.bam -f BAM -g 12100000 -n SRX4554691.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4554691.20 # format = BAM # ChIP-seq file = ['SRX4554691.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:01:37: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:01:37: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:01:37: # Command line: callpeak -t SRX4554691.bam -f BAM -g 12100000 -n SRX4554691.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4554691.05 # format = BAM # ChIP-seq file = ['SRX4554691.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 23:01:37: #1 read tag files... INFO @ Tue, 09 Oct 2018 23:01:37: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 23:01:42: 1000000 INFO @ Tue, 09 Oct 2018 23:01:43: 1000000 INFO @ Tue, 09 Oct 2018 23:01:43: 1000000 INFO @ Tue, 09 Oct 2018 23:01:48: 2000000 INFO @ Tue, 09 Oct 2018 23:01:48: 2000000 INFO @ Tue, 09 Oct 2018 23:01:48: 2000000 INFO @ Tue, 09 Oct 2018 23:01:53: 3000000 INFO @ Tue, 09 Oct 2018 23:01:53: 3000000 INFO @ Tue, 09 Oct 2018 23:01:54: 3000000 INFO @ Tue, 09 Oct 2018 23:01:56: #1 tag size is determined as 37 bps INFO @ Tue, 09 Oct 2018 23:01:56: #1 tag size = 37 INFO @ Tue, 09 Oct 2018 23:01:56: #1 total tags in treatment: 665517 INFO @ Tue, 09 Oct 2018 23:01:56: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:01:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:01:56: #1 tags after filtering in treatment: 449805 INFO @ Tue, 09 Oct 2018 23:01:56: #1 Redundant rate of treatment: 0.32 INFO @ Tue, 09 Oct 2018 23:01:56: #1 finished! INFO @ Tue, 09 Oct 2018 23:01:56: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:01:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:01:56: #2 number of paired peaks: 302 WARNING @ Tue, 09 Oct 2018 23:01:56: Fewer paired peaks (302) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 302 pairs to build model! INFO @ Tue, 09 Oct 2018 23:01:56: start model_add_line... INFO @ Tue, 09 Oct 2018 23:01:56: start X-correlation... INFO @ Tue, 09 Oct 2018 23:01:56: end of X-cor INFO @ Tue, 09 Oct 2018 23:01:56: #2 finished! INFO @ Tue, 09 Oct 2018 23:01:56: #2 predicted fragment length is 194 bps INFO @ Tue, 09 Oct 2018 23:01:56: #2 alternative fragment length(s) may be 3,194,221,237,259,547,586,593,596 bps INFO @ Tue, 09 Oct 2018 23:01:56: #2.2 Generate R script for model : SRX4554691.05_model.r INFO @ Tue, 09 Oct 2018 23:01:56: #3 Call peaks... INFO @ Tue, 09 Oct 2018 23:01:56: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 09 Oct 2018 23:01:56: #1 tag size is determined as 37 bps INFO @ Tue, 09 Oct 2018 23:01:56: #1 tag size = 37 INFO @ Tue, 09 Oct 2018 23:01:56: #1 total tags in treatment: 665517 INFO @ Tue, 09 Oct 2018 23:01:56: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:01:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:01:56: #1 tags after filtering in treatment: 449805 INFO @ Tue, 09 Oct 2018 23:01:56: #1 Redundant rate of treatment: 0.32 INFO @ Tue, 09 Oct 2018 23:01:56: #1 finished! INFO @ Tue, 09 Oct 2018 23:01:56: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:01:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:01:56: #1 tag size is determined as 37 bps INFO @ Tue, 09 Oct 2018 23:01:56: #1 tag size = 37 INFO @ Tue, 09 Oct 2018 23:01:56: #1 total tags in treatment: 665517 INFO @ Tue, 09 Oct 2018 23:01:56: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 23:01:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 23:01:56: #1 tags after filtering in treatment: 449805 INFO @ Tue, 09 Oct 2018 23:01:56: #1 Redundant rate of treatment: 0.32 INFO @ Tue, 09 Oct 2018 23:01:56: #1 finished! INFO @ Tue, 09 Oct 2018 23:01:56: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 23:01:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 23:01:57: #2 number of paired peaks: 302 WARNING @ Tue, 09 Oct 2018 23:01:57: Fewer paired peaks (302) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 302 pairs to build model! INFO @ Tue, 09 Oct 2018 23:01:57: start model_add_line... INFO @ Tue, 09 Oct 2018 23:01:57: start X-correlation... INFO @ Tue, 09 Oct 2018 23:01:57: end of X-cor INFO @ Tue, 09 Oct 2018 23:01:57: #2 finished! INFO @ Tue, 09 Oct 2018 23:01:57: #2 predicted fragment length is 194 bps INFO @ Tue, 09 Oct 2018 23:01:57: #2 alternative fragment length(s) may be 3,194,221,237,259,547,586,593,596 bps INFO @ Tue, 09 Oct 2018 23:01:57: #2.2 Generate R script for model : SRX4554691.10_model.r INFO @ Tue, 09 Oct 2018 23:01:57: #3 Call peaks... INFO @ Tue, 09 Oct 2018 23:01:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 09 Oct 2018 23:01:57: #2 number of paired peaks: 302 WARNING @ Tue, 09 Oct 2018 23:01:57: Fewer paired peaks (302) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 302 pairs to build model! INFO @ Tue, 09 Oct 2018 23:01:57: start model_add_line... INFO @ Tue, 09 Oct 2018 23:01:57: start X-correlation... INFO @ Tue, 09 Oct 2018 23:01:57: end of X-cor INFO @ Tue, 09 Oct 2018 23:01:57: #2 finished! INFO @ Tue, 09 Oct 2018 23:01:57: #2 predicted fragment length is 194 bps INFO @ Tue, 09 Oct 2018 23:01:57: #2 alternative fragment length(s) may be 3,194,221,237,259,547,586,593,596 bps INFO @ Tue, 09 Oct 2018 23:01:57: #2.2 Generate R script for model : SRX4554691.20_model.r INFO @ Tue, 09 Oct 2018 23:01:57: #3 Call peaks... INFO @ Tue, 09 Oct 2018 23:01:57: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 09 Oct 2018 23:01:58: #3 Call peaks for each chromosome... INFO @ Tue, 09 Oct 2018 23:01:58: #4 Write output xls file... SRX4554691.05_peaks.xls INFO @ Tue, 09 Oct 2018 23:01:58: #4 Write peak in narrowPeak format file... SRX4554691.05_peaks.narrowPeak INFO @ Tue, 09 Oct 2018 23:01:58: #4 Write summits bed file... SRX4554691.05_summits.bed INFO @ Tue, 09 Oct 2018 23:01:58: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (22 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:01:58: #3 Call peaks for each chromosome... INFO @ Tue, 09 Oct 2018 23:01:58: #3 Call peaks for each chromosome... INFO @ Tue, 09 Oct 2018 23:01:59: #4 Write output xls file... SRX4554691.10_peaks.xls INFO @ Tue, 09 Oct 2018 23:01:59: #4 Write peak in narrowPeak format file... SRX4554691.10_peaks.narrowPeak INFO @ Tue, 09 Oct 2018 23:01:59: #4 Write summits bed file... SRX4554691.10_summits.bed INFO @ Tue, 09 Oct 2018 23:01:59: Done! pass1 - making usageList (3 chroms): 1 millis pass2 - checking and writing primary data (6 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 23:01:59: #4 Write output xls file... SRX4554691.20_peaks.xls INFO @ Tue, 09 Oct 2018 23:01:59: #4 Write peak in narrowPeak format file... SRX4554691.20_peaks.narrowPeak INFO @ Tue, 09 Oct 2018 23:01:59: #4 Write summits bed file... SRX4554691.20_summits.bed INFO @ Tue, 09 Oct 2018 23:01:59: Done! pass1 - making usageList (1 chroms): 1 millis pass2 - checking and writing primary data (2 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。