Job ID = 11244908 sra ファイルのダウンロード中... Completed: 146458K bytes transferred in 6 seconds (195956K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 4949792 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4554655/SRR7696329.sra Written 4949792 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4554655/SRR7696329.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 4949792 reads; of these: 4949792 (100.00%) were paired; of these: 3272139 (66.11%) aligned concordantly 0 times 1425649 (28.80%) aligned concordantly exactly 1 time 252004 (5.09%) aligned concordantly >1 times ---- 3272139 pairs aligned concordantly 0 times; of these: 23275 (0.71%) aligned discordantly 1 time ---- 3248864 pairs aligned 0 times concordantly or discordantly; of these: 6497728 mates make up the pairs; of these: 4486565 (69.05%) aligned 0 times 1712004 (26.35%) aligned exactly 1 time 299159 (4.60%) aligned >1 times 54.68% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 77667 / 1700438 = 0.0457 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 09 Oct 2018 22:48:32: # Command line: callpeak -t SRX4554655.bam -f BAM -g 12100000 -n SRX4554655.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4554655.20 # format = BAM # ChIP-seq file = ['SRX4554655.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 22:48:32: #1 read tag files... INFO @ Tue, 09 Oct 2018 22:48:32: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 22:48:32: # Command line: callpeak -t SRX4554655.bam -f BAM -g 12100000 -n SRX4554655.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4554655.10 # format = BAM # ChIP-seq file = ['SRX4554655.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 22:48:32: #1 read tag files... INFO @ Tue, 09 Oct 2018 22:48:32: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 22:48:32: # Command line: callpeak -t SRX4554655.bam -f BAM -g 12100000 -n SRX4554655.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4554655.05 # format = BAM # ChIP-seq file = ['SRX4554655.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 09 Oct 2018 22:48:32: #1 read tag files... INFO @ Tue, 09 Oct 2018 22:48:32: #1 read treatment tags... INFO @ Tue, 09 Oct 2018 22:48:38: 1000000 INFO @ Tue, 09 Oct 2018 22:48:38: 1000000 INFO @ Tue, 09 Oct 2018 22:48:38: 1000000 INFO @ Tue, 09 Oct 2018 22:48:43: 2000000 INFO @ Tue, 09 Oct 2018 22:48:44: 2000000 INFO @ Tue, 09 Oct 2018 22:48:44: 2000000 INFO @ Tue, 09 Oct 2018 22:48:49: 3000000 INFO @ Tue, 09 Oct 2018 22:48:50: 3000000 INFO @ Tue, 09 Oct 2018 22:48:50: 3000000 INFO @ Tue, 09 Oct 2018 22:48:55: 4000000 INFO @ Tue, 09 Oct 2018 22:48:56: 4000000 INFO @ Tue, 09 Oct 2018 22:48:56: 4000000 INFO @ Tue, 09 Oct 2018 22:49:01: 5000000 INFO @ Tue, 09 Oct 2018 22:49:02: 5000000 INFO @ Tue, 09 Oct 2018 22:49:02: 5000000 INFO @ Tue, 09 Oct 2018 22:49:02: #1 tag size is determined as 37 bps INFO @ Tue, 09 Oct 2018 22:49:02: #1 tag size = 37 INFO @ Tue, 09 Oct 2018 22:49:02: #1 total tags in treatment: 1600428 INFO @ Tue, 09 Oct 2018 22:49:02: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 22:49:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 22:49:02: #1 tags after filtering in treatment: 1189006 INFO @ Tue, 09 Oct 2018 22:49:02: #1 Redundant rate of treatment: 0.26 INFO @ Tue, 09 Oct 2018 22:49:02: #1 finished! INFO @ Tue, 09 Oct 2018 22:49:02: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 22:49:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 22:49:02: #2 number of paired peaks: 389 WARNING @ Tue, 09 Oct 2018 22:49:02: Fewer paired peaks (389) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 389 pairs to build model! INFO @ Tue, 09 Oct 2018 22:49:02: start model_add_line... INFO @ Tue, 09 Oct 2018 22:49:02: start X-correlation... INFO @ Tue, 09 Oct 2018 22:49:02: end of X-cor INFO @ Tue, 09 Oct 2018 22:49:02: #2 finished! INFO @ Tue, 09 Oct 2018 22:49:02: #2 predicted fragment length is 260 bps INFO @ Tue, 09 Oct 2018 22:49:02: #2 alternative fragment length(s) may be 1,241,260 bps INFO @ Tue, 09 Oct 2018 22:49:02: #2.2 Generate R script for model : SRX4554655.05_model.r INFO @ Tue, 09 Oct 2018 22:49:02: #3 Call peaks... INFO @ Tue, 09 Oct 2018 22:49:02: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 09 Oct 2018 22:49:03: #1 tag size is determined as 37 bps INFO @ Tue, 09 Oct 2018 22:49:03: #1 tag size = 37 INFO @ Tue, 09 Oct 2018 22:49:03: #1 total tags in treatment: 1600428 INFO @ Tue, 09 Oct 2018 22:49:03: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 22:49:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 22:49:03: #1 tag size is determined as 37 bps INFO @ Tue, 09 Oct 2018 22:49:03: #1 tag size = 37 INFO @ Tue, 09 Oct 2018 22:49:03: #1 total tags in treatment: 1600428 INFO @ Tue, 09 Oct 2018 22:49:03: #1 user defined the maximum tags... INFO @ Tue, 09 Oct 2018 22:49:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 09 Oct 2018 22:49:03: #1 tags after filtering in treatment: 1189006 INFO @ Tue, 09 Oct 2018 22:49:03: #1 Redundant rate of treatment: 0.26 INFO @ Tue, 09 Oct 2018 22:49:03: #1 finished! INFO @ Tue, 09 Oct 2018 22:49:03: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 22:49:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 22:49:03: #1 tags after filtering in treatment: 1189006 INFO @ Tue, 09 Oct 2018 22:49:03: #1 Redundant rate of treatment: 0.26 INFO @ Tue, 09 Oct 2018 22:49:03: #1 finished! INFO @ Tue, 09 Oct 2018 22:49:03: #2 Build Peak Model... INFO @ Tue, 09 Oct 2018 22:49:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 09 Oct 2018 22:49:03: #2 number of paired peaks: 389 WARNING @ Tue, 09 Oct 2018 22:49:03: Fewer paired peaks (389) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 389 pairs to build model! INFO @ Tue, 09 Oct 2018 22:49:03: start model_add_line... INFO @ Tue, 09 Oct 2018 22:49:03: #2 number of paired peaks: 389 WARNING @ Tue, 09 Oct 2018 22:49:03: Fewer paired peaks (389) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 389 pairs to build model! INFO @ Tue, 09 Oct 2018 22:49:03: start model_add_line... INFO @ Tue, 09 Oct 2018 22:49:03: start X-correlation... INFO @ Tue, 09 Oct 2018 22:49:03: end of X-cor INFO @ Tue, 09 Oct 2018 22:49:03: #2 finished! INFO @ Tue, 09 Oct 2018 22:49:03: #2 predicted fragment length is 260 bps INFO @ Tue, 09 Oct 2018 22:49:03: #2 alternative fragment length(s) may be 1,241,260 bps INFO @ Tue, 09 Oct 2018 22:49:03: #2.2 Generate R script for model : SRX4554655.20_model.r INFO @ Tue, 09 Oct 2018 22:49:03: #3 Call peaks... INFO @ Tue, 09 Oct 2018 22:49:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 09 Oct 2018 22:49:03: start X-correlation... INFO @ Tue, 09 Oct 2018 22:49:03: end of X-cor INFO @ Tue, 09 Oct 2018 22:49:03: #2 finished! INFO @ Tue, 09 Oct 2018 22:49:03: #2 predicted fragment length is 260 bps INFO @ Tue, 09 Oct 2018 22:49:03: #2 alternative fragment length(s) may be 1,241,260 bps INFO @ Tue, 09 Oct 2018 22:49:03: #2.2 Generate R script for model : SRX4554655.10_model.r INFO @ Tue, 09 Oct 2018 22:49:03: #3 Call peaks... INFO @ Tue, 09 Oct 2018 22:49:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 09 Oct 2018 22:49:09: #3 Call peaks for each chromosome... INFO @ Tue, 09 Oct 2018 22:49:10: #3 Call peaks for each chromosome... INFO @ Tue, 09 Oct 2018 22:49:10: #3 Call peaks for each chromosome... INFO @ Tue, 09 Oct 2018 22:49:10: #4 Write output xls file... SRX4554655.05_peaks.xls INFO @ Tue, 09 Oct 2018 22:49:10: #4 Write peak in narrowPeak format file... SRX4554655.05_peaks.narrowPeak INFO @ Tue, 09 Oct 2018 22:49:10: #4 Write summits bed file... SRX4554655.05_summits.bed INFO @ Tue, 09 Oct 2018 22:49:10: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (262 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 09 Oct 2018 22:49:11: #4 Write output xls file... SRX4554655.10_peaks.xls INFO @ Tue, 09 Oct 2018 22:49:11: #4 Write peak in narrowPeak format file... SRX4554655.10_peaks.narrowPeak INFO @ Tue, 09 Oct 2018 22:49:11: #4 Write summits bed file... SRX4554655.10_summits.bed INFO @ Tue, 09 Oct 2018 22:49:11: #4 Write output xls file... SRX4554655.20_peaks.xls INFO @ Tue, 09 Oct 2018 22:49:11: Done! INFO @ Tue, 09 Oct 2018 22:49:11: #4 Write peak in narrowPeak format file... SRX4554655.20_peaks.narrowPeak INFO @ Tue, 09 Oct 2018 22:49:11: #4 Write summits bed file... SRX4554655.20_summits.bed INFO @ Tue, 09 Oct 2018 22:49:11: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (216 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (17 chroms): 0 millis pass2 - checking and writing primary data (190 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。