Job ID = 2011062 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,413,846 reads read : 26,413,846 reads written : 26,413,846 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:14 26413846 reads; of these: 26413846 (100.00%) were unpaired; of these: 952112 (3.60%) aligned 0 times 21811452 (82.58%) aligned exactly 1 time 3650282 (13.82%) aligned >1 times 96.40% overall alignment rate Time searching: 00:05:14 Overall time: 00:05:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11484166 / 25461734 = 0.4510 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:14:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:57: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:57: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:58: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:58: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:59: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:59: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:15:07: 1000000 INFO @ Sat, 06 Jul 2019 02:15:08: 1000000 INFO @ Sat, 06 Jul 2019 02:15:08: 1000000 INFO @ Sat, 06 Jul 2019 02:15:16: 2000000 INFO @ Sat, 06 Jul 2019 02:15:17: 2000000 INFO @ Sat, 06 Jul 2019 02:15:18: 2000000 INFO @ Sat, 06 Jul 2019 02:15:27: 3000000 INFO @ Sat, 06 Jul 2019 02:15:27: 3000000 INFO @ Sat, 06 Jul 2019 02:15:27: 3000000 INFO @ Sat, 06 Jul 2019 02:15:36: 4000000 INFO @ Sat, 06 Jul 2019 02:15:38: 4000000 INFO @ Sat, 06 Jul 2019 02:15:38: 4000000 INFO @ Sat, 06 Jul 2019 02:15:45: 5000000 INFO @ Sat, 06 Jul 2019 02:15:48: 5000000 INFO @ Sat, 06 Jul 2019 02:15:48: 5000000 INFO @ Sat, 06 Jul 2019 02:15:54: 6000000 INFO @ Sat, 06 Jul 2019 02:15:59: 6000000 INFO @ Sat, 06 Jul 2019 02:15:59: 6000000 INFO @ Sat, 06 Jul 2019 02:16:03: 7000000 INFO @ Sat, 06 Jul 2019 02:16:08: 7000000 INFO @ Sat, 06 Jul 2019 02:16:09: 7000000 INFO @ Sat, 06 Jul 2019 02:16:12: 8000000 INFO @ Sat, 06 Jul 2019 02:16:18: 8000000 INFO @ Sat, 06 Jul 2019 02:16:19: 8000000 INFO @ Sat, 06 Jul 2019 02:16:20: 9000000 INFO @ Sat, 06 Jul 2019 02:16:28: 9000000 INFO @ Sat, 06 Jul 2019 02:16:29: 10000000 INFO @ Sat, 06 Jul 2019 02:16:29: 9000000 INFO @ Sat, 06 Jul 2019 02:16:38: 10000000 INFO @ Sat, 06 Jul 2019 02:16:38: 11000000 INFO @ Sat, 06 Jul 2019 02:16:39: 10000000 INFO @ Sat, 06 Jul 2019 02:16:47: 12000000 INFO @ Sat, 06 Jul 2019 02:16:48: 11000000 INFO @ Sat, 06 Jul 2019 02:16:50: 11000000 INFO @ Sat, 06 Jul 2019 02:16:56: 13000000 INFO @ Sat, 06 Jul 2019 02:16:58: 12000000 INFO @ Sat, 06 Jul 2019 02:17:00: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 02:17:05: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:17:05: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:17:05: #1 total tags in treatment: 13977568 INFO @ Sat, 06 Jul 2019 02:17:05: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:17:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:17:05: #1 tags after filtering in treatment: 13977568 INFO @ Sat, 06 Jul 2019 02:17:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:17:05: #1 finished! INFO @ Sat, 06 Jul 2019 02:17:05: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:17:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:17:06: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:17:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:17:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:17:08: 13000000 INFO @ Sat, 06 Jul 2019 02:17:11: 13000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 02:17:18: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:17:18: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:17:18: #1 total tags in treatment: 13977568 INFO @ Sat, 06 Jul 2019 02:17:18: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:17:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:17:18: #1 tags after filtering in treatment: 13977568 INFO @ Sat, 06 Jul 2019 02:17:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:17:18: #1 finished! INFO @ Sat, 06 Jul 2019 02:17:18: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:17:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:17:19: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:17:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:17:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:17:21: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:17:21: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:17:21: #1 total tags in treatment: 13977568 INFO @ Sat, 06 Jul 2019 02:17:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:17:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:17:21: #1 tags after filtering in treatment: 13977568 INFO @ Sat, 06 Jul 2019 02:17:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:17:21: #1 finished! INFO @ Sat, 06 Jul 2019 02:17:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:17:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:17:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:17:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:17:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553749/SRX4553749.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling