Job ID = 2011060 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,693,370 reads read : 26,693,370 reads written : 26,693,370 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:37 26693370 reads; of these: 26693370 (100.00%) were unpaired; of these: 1199006 (4.49%) aligned 0 times 21864980 (81.91%) aligned exactly 1 time 3629384 (13.60%) aligned >1 times 95.51% overall alignment rate Time searching: 00:04:37 Overall time: 00:04:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11581057 / 25494364 = 0.4543 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:18:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:18:39: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:18:39: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:18:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:18:39: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:18:39: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:18:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:18:40: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:18:40: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:18:47: 1000000 INFO @ Sat, 06 Jul 2019 02:18:47: 1000000 INFO @ Sat, 06 Jul 2019 02:18:47: 1000000 INFO @ Sat, 06 Jul 2019 02:18:54: 2000000 INFO @ Sat, 06 Jul 2019 02:18:54: 2000000 INFO @ Sat, 06 Jul 2019 02:18:55: 2000000 INFO @ Sat, 06 Jul 2019 02:19:01: 3000000 INFO @ Sat, 06 Jul 2019 02:19:02: 3000000 INFO @ Sat, 06 Jul 2019 02:19:02: 3000000 INFO @ Sat, 06 Jul 2019 02:19:08: 4000000 INFO @ Sat, 06 Jul 2019 02:19:09: 4000000 INFO @ Sat, 06 Jul 2019 02:19:09: 4000000 INFO @ Sat, 06 Jul 2019 02:19:14: 5000000 INFO @ Sat, 06 Jul 2019 02:19:16: 5000000 INFO @ Sat, 06 Jul 2019 02:19:17: 5000000 INFO @ Sat, 06 Jul 2019 02:19:21: 6000000 INFO @ Sat, 06 Jul 2019 02:19:24: 6000000 INFO @ Sat, 06 Jul 2019 02:19:24: 6000000 INFO @ Sat, 06 Jul 2019 02:19:28: 7000000 INFO @ Sat, 06 Jul 2019 02:19:31: 7000000 INFO @ Sat, 06 Jul 2019 02:19:32: 7000000 INFO @ Sat, 06 Jul 2019 02:19:35: 8000000 INFO @ Sat, 06 Jul 2019 02:19:39: 8000000 INFO @ Sat, 06 Jul 2019 02:19:39: 8000000 INFO @ Sat, 06 Jul 2019 02:19:42: 9000000 INFO @ Sat, 06 Jul 2019 02:19:47: 9000000 INFO @ Sat, 06 Jul 2019 02:19:47: 9000000 INFO @ Sat, 06 Jul 2019 02:19:48: 10000000 INFO @ Sat, 06 Jul 2019 02:19:54: 10000000 INFO @ Sat, 06 Jul 2019 02:19:55: 10000000 INFO @ Sat, 06 Jul 2019 02:19:55: 11000000 INFO @ Sat, 06 Jul 2019 02:20:01: 11000000 INFO @ Sat, 06 Jul 2019 02:20:02: 12000000 INFO @ Sat, 06 Jul 2019 02:20:02: 11000000 INFO @ Sat, 06 Jul 2019 02:20:09: 13000000 INFO @ Sat, 06 Jul 2019 02:20:09: 12000000 INFO @ Sat, 06 Jul 2019 02:20:10: 12000000 INFO @ Sat, 06 Jul 2019 02:20:15: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:20:15: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:20:15: #1 total tags in treatment: 13913307 INFO @ Sat, 06 Jul 2019 02:20:15: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:20:15: #1 tags after filtering in treatment: 13913307 INFO @ Sat, 06 Jul 2019 02:20:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:20:15: #1 finished! INFO @ Sat, 06 Jul 2019 02:20:15: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:20:16: 13000000 INFO @ Sat, 06 Jul 2019 02:20:16: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:20:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:20:16: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:20:17: 13000000 INFO @ Sat, 06 Jul 2019 02:20:22: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:20:22: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:20:22: #1 total tags in treatment: 13913307 INFO @ Sat, 06 Jul 2019 02:20:22: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:20:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:20:23: #1 tags after filtering in treatment: 13913307 INFO @ Sat, 06 Jul 2019 02:20:23: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:20:23: #1 finished! INFO @ Sat, 06 Jul 2019 02:20:23: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:20:23: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:20:24: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:20:24: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:20:24: #1 total tags in treatment: 13913307 INFO @ Sat, 06 Jul 2019 02:20:24: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:20:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:20:24: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:20:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:20:24: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:20:24: #1 tags after filtering in treatment: 13913307 INFO @ Sat, 06 Jul 2019 02:20:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:20:24: #1 finished! INFO @ Sat, 06 Jul 2019 02:20:24: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:20:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:20:25: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:20:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:20:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553747/SRX4553747.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。