Job ID = 2011056 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,663,080 reads read : 28,663,080 reads written : 28,663,080 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:48 28663080 reads; of these: 28663080 (100.00%) were unpaired; of these: 1458183 (5.09%) aligned 0 times 25130903 (87.68%) aligned exactly 1 time 2073994 (7.24%) aligned >1 times 94.91% overall alignment rate Time searching: 00:04:48 Overall time: 00:04:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14897909 / 27204897 = 0.5476 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:14:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:27: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:27: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:28: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:28: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:34: 1000000 INFO @ Sat, 06 Jul 2019 02:14:35: 1000000 INFO @ Sat, 06 Jul 2019 02:14:36: 1000000 INFO @ Sat, 06 Jul 2019 02:14:42: 2000000 INFO @ Sat, 06 Jul 2019 02:14:42: 2000000 INFO @ Sat, 06 Jul 2019 02:14:43: 2000000 INFO @ Sat, 06 Jul 2019 02:14:49: 3000000 INFO @ Sat, 06 Jul 2019 02:14:50: 3000000 INFO @ Sat, 06 Jul 2019 02:14:50: 3000000 INFO @ Sat, 06 Jul 2019 02:14:56: 4000000 INFO @ Sat, 06 Jul 2019 02:14:58: 4000000 INFO @ Sat, 06 Jul 2019 02:14:58: 4000000 INFO @ Sat, 06 Jul 2019 02:15:03: 5000000 INFO @ Sat, 06 Jul 2019 02:15:05: 5000000 INFO @ Sat, 06 Jul 2019 02:15:06: 5000000 INFO @ Sat, 06 Jul 2019 02:15:09: 6000000 INFO @ Sat, 06 Jul 2019 02:15:12: 6000000 INFO @ Sat, 06 Jul 2019 02:15:14: 6000000 INFO @ Sat, 06 Jul 2019 02:15:16: 7000000 INFO @ Sat, 06 Jul 2019 02:15:19: 7000000 INFO @ Sat, 06 Jul 2019 02:15:22: 7000000 INFO @ Sat, 06 Jul 2019 02:15:23: 8000000 INFO @ Sat, 06 Jul 2019 02:15:26: 8000000 INFO @ Sat, 06 Jul 2019 02:15:30: 9000000 INFO @ Sat, 06 Jul 2019 02:15:30: 8000000 INFO @ Sat, 06 Jul 2019 02:15:33: 9000000 INFO @ Sat, 06 Jul 2019 02:15:36: 10000000 INFO @ Sat, 06 Jul 2019 02:15:39: 9000000 INFO @ Sat, 06 Jul 2019 02:15:40: 10000000 INFO @ Sat, 06 Jul 2019 02:15:43: 11000000 INFO @ Sat, 06 Jul 2019 02:15:47: 10000000 INFO @ Sat, 06 Jul 2019 02:15:47: 11000000 INFO @ Sat, 06 Jul 2019 02:15:50: 12000000 INFO @ Sat, 06 Jul 2019 02:15:52: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:15:52: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:15:52: #1 total tags in treatment: 12306988 INFO @ Sat, 06 Jul 2019 02:15:52: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:15:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:15:52: #1 tags after filtering in treatment: 12306988 INFO @ Sat, 06 Jul 2019 02:15:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:15:52: #1 finished! INFO @ Sat, 06 Jul 2019 02:15:52: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:15:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:15:53: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:15:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:15:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:15:54: 12000000 INFO @ Sat, 06 Jul 2019 02:15:55: 11000000 INFO @ Sat, 06 Jul 2019 02:15:56: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:15:56: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:15:56: #1 total tags in treatment: 12306988 INFO @ Sat, 06 Jul 2019 02:15:56: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:15:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:15:56: #1 tags after filtering in treatment: 12306988 INFO @ Sat, 06 Jul 2019 02:15:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:15:56: #1 finished! INFO @ Sat, 06 Jul 2019 02:15:56: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:15:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:15:57: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:15:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:15:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:16:03: 12000000 INFO @ Sat, 06 Jul 2019 02:16:05: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:16:05: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:16:05: #1 total tags in treatment: 12306988 INFO @ Sat, 06 Jul 2019 02:16:05: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:16:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:16:05: #1 tags after filtering in treatment: 12306988 INFO @ Sat, 06 Jul 2019 02:16:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:16:05: #1 finished! INFO @ Sat, 06 Jul 2019 02:16:05: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:16:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:16:06: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:16:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:16:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553743/SRX4553743.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。