Job ID = 2011054 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,833,109 reads read : 26,833,109 reads written : 26,833,109 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:56 26833109 reads; of these: 26833109 (100.00%) were unpaired; of these: 1858931 (6.93%) aligned 0 times 22829482 (85.08%) aligned exactly 1 time 2144696 (7.99%) aligned >1 times 93.07% overall alignment rate Time searching: 00:04:56 Overall time: 00:04:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13208604 / 24974178 = 0.5289 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:14:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:16: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:16: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:17: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:17: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:25: 1000000 INFO @ Sat, 06 Jul 2019 02:14:26: 1000000 INFO @ Sat, 06 Jul 2019 02:14:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:14:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:14:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:14:33: 2000000 INFO @ Sat, 06 Jul 2019 02:14:34: 2000000 INFO @ Sat, 06 Jul 2019 02:14:39: 1000000 INFO @ Sat, 06 Jul 2019 02:14:41: 3000000 INFO @ Sat, 06 Jul 2019 02:14:42: 3000000 INFO @ Sat, 06 Jul 2019 02:14:49: 4000000 INFO @ Sat, 06 Jul 2019 02:14:49: 2000000 INFO @ Sat, 06 Jul 2019 02:14:50: 4000000 INFO @ Sat, 06 Jul 2019 02:14:57: 5000000 INFO @ Sat, 06 Jul 2019 02:14:58: 5000000 INFO @ Sat, 06 Jul 2019 02:14:59: 3000000 INFO @ Sat, 06 Jul 2019 02:15:05: 6000000 INFO @ Sat, 06 Jul 2019 02:15:06: 6000000 INFO @ Sat, 06 Jul 2019 02:15:08: 4000000 INFO @ Sat, 06 Jul 2019 02:15:13: 7000000 INFO @ Sat, 06 Jul 2019 02:15:14: 7000000 INFO @ Sat, 06 Jul 2019 02:15:18: 5000000 INFO @ Sat, 06 Jul 2019 02:15:21: 8000000 INFO @ Sat, 06 Jul 2019 02:15:22: 8000000 INFO @ Sat, 06 Jul 2019 02:15:27: 6000000 INFO @ Sat, 06 Jul 2019 02:15:29: 9000000 INFO @ Sat, 06 Jul 2019 02:15:30: 9000000 INFO @ Sat, 06 Jul 2019 02:15:37: 7000000 INFO @ Sat, 06 Jul 2019 02:15:37: 10000000 INFO @ Sat, 06 Jul 2019 02:15:38: 10000000 INFO @ Sat, 06 Jul 2019 02:15:45: 11000000 INFO @ Sat, 06 Jul 2019 02:15:46: 11000000 INFO @ Sat, 06 Jul 2019 02:15:46: 8000000 INFO @ Sat, 06 Jul 2019 02:15:51: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:15:51: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:15:51: #1 total tags in treatment: 11765574 INFO @ Sat, 06 Jul 2019 02:15:51: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:15:51: #1 tags after filtering in treatment: 11765574 INFO @ Sat, 06 Jul 2019 02:15:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:15:51: #1 finished! INFO @ Sat, 06 Jul 2019 02:15:51: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:15:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:15:52: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:15:52: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:15:52: #1 total tags in treatment: 11765574 INFO @ Sat, 06 Jul 2019 02:15:52: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:15:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:15:52: #1 tags after filtering in treatment: 11765574 INFO @ Sat, 06 Jul 2019 02:15:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:15:52: #1 finished! INFO @ Sat, 06 Jul 2019 02:15:52: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:15:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:15:52: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:15:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:15:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:15:53: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:15:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:15:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:15:56: 9000000 INFO @ Sat, 06 Jul 2019 02:16:05: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 02:16:14: 11000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 02:16:21: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:16:21: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:16:21: #1 total tags in treatment: 11765574 INFO @ Sat, 06 Jul 2019 02:16:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:16:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:16:21: #1 tags after filtering in treatment: 11765574 INFO @ Sat, 06 Jul 2019 02:16:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:16:21: #1 finished! INFO @ Sat, 06 Jul 2019 02:16:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:16:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:16:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:16:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:16:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553741/SRX4553741.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling