Job ID = 2011050 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 25,539,487 reads read : 25,539,487 reads written : 25,539,487 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:00 25539487 reads; of these: 25539487 (100.00%) were unpaired; of these: 1425853 (5.58%) aligned 0 times 21455654 (84.01%) aligned exactly 1 time 2657980 (10.41%) aligned >1 times 94.42% overall alignment rate Time searching: 00:05:00 Overall time: 00:05:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12834430 / 24113634 = 0.5322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:10:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:10:40: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:10:40: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:10:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:10:41: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:10:41: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:10:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:10:42: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:10:42: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:10:48: 1000000 INFO @ Sat, 06 Jul 2019 02:10:50: 1000000 INFO @ Sat, 06 Jul 2019 02:10:53: 1000000 INFO @ Sat, 06 Jul 2019 02:10:55: 2000000 INFO @ Sat, 06 Jul 2019 02:10:58: 2000000 INFO @ Sat, 06 Jul 2019 02:11:03: 3000000 INFO @ Sat, 06 Jul 2019 02:11:03: 2000000 INFO @ Sat, 06 Jul 2019 02:11:06: 3000000 INFO @ Sat, 06 Jul 2019 02:11:10: 4000000 INFO @ Sat, 06 Jul 2019 02:11:14: 3000000 INFO @ Sat, 06 Jul 2019 02:11:14: 4000000 INFO @ Sat, 06 Jul 2019 02:11:18: 5000000 INFO @ Sat, 06 Jul 2019 02:11:23: 5000000 INFO @ Sat, 06 Jul 2019 02:11:24: 4000000 INFO @ Sat, 06 Jul 2019 02:11:25: 6000000 INFO @ Sat, 06 Jul 2019 02:11:31: 6000000 INFO @ Sat, 06 Jul 2019 02:11:33: 7000000 INFO @ Sat, 06 Jul 2019 02:11:34: 5000000 INFO @ Sat, 06 Jul 2019 02:11:39: 7000000 INFO @ Sat, 06 Jul 2019 02:11:40: 8000000 INFO @ Sat, 06 Jul 2019 02:11:45: 6000000 INFO @ Sat, 06 Jul 2019 02:11:47: 8000000 INFO @ Sat, 06 Jul 2019 02:11:48: 9000000 INFO @ Sat, 06 Jul 2019 02:11:55: 10000000 INFO @ Sat, 06 Jul 2019 02:11:55: 7000000 INFO @ Sat, 06 Jul 2019 02:11:56: 9000000 INFO @ Sat, 06 Jul 2019 02:12:02: 11000000 INFO @ Sat, 06 Jul 2019 02:12:04: 10000000 INFO @ Sat, 06 Jul 2019 02:12:05: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:12:05: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:12:05: #1 total tags in treatment: 11279204 INFO @ Sat, 06 Jul 2019 02:12:05: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:12:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:12:05: #1 tags after filtering in treatment: 11279204 INFO @ Sat, 06 Jul 2019 02:12:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:12:05: #1 finished! INFO @ Sat, 06 Jul 2019 02:12:05: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:12:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:12:05: 8000000 INFO @ Sat, 06 Jul 2019 02:12:06: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:12:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:12:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:12:12: 11000000 INFO @ Sat, 06 Jul 2019 02:12:14: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:12:14: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:12:14: #1 total tags in treatment: 11279204 INFO @ Sat, 06 Jul 2019 02:12:14: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:12:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:12:14: #1 tags after filtering in treatment: 11279204 INFO @ Sat, 06 Jul 2019 02:12:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:12:14: #1 finished! INFO @ Sat, 06 Jul 2019 02:12:14: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:12:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:12:15: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:12:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:12:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:12:15: 9000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 02:12:25: 10000000 INFO @ Sat, 06 Jul 2019 02:12:35: 11000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 02:12:38: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:12:38: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:12:38: #1 total tags in treatment: 11279204 INFO @ Sat, 06 Jul 2019 02:12:38: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:12:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:12:38: #1 tags after filtering in treatment: 11279204 INFO @ Sat, 06 Jul 2019 02:12:38: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:12:38: #1 finished! INFO @ Sat, 06 Jul 2019 02:12:38: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:12:38: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:12:39: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:12:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:12:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553738/SRX4553738.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling