Job ID = 2011048 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,861,627 reads read : 24,861,627 reads written : 24,861,627 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:16 24861627 reads; of these: 24861627 (100.00%) were unpaired; of these: 1743543 (7.01%) aligned 0 times 20535826 (82.60%) aligned exactly 1 time 2582258 (10.39%) aligned >1 times 92.99% overall alignment rate Time searching: 00:04:16 Overall time: 00:04:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12075073 / 23118084 = 0.5223 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:13:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:13:50: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:13:50: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:13:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:13:51: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:13:51: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:13:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:13:52: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:13:52: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:13:59: 1000000 INFO @ Sat, 06 Jul 2019 02:13:59: 1000000 INFO @ Sat, 06 Jul 2019 02:14:01: 1000000 INFO @ Sat, 06 Jul 2019 02:14:08: 2000000 INFO @ Sat, 06 Jul 2019 02:14:08: 2000000 INFO @ Sat, 06 Jul 2019 02:14:10: 2000000 INFO @ Sat, 06 Jul 2019 02:14:18: 3000000 INFO @ Sat, 06 Jul 2019 02:14:18: 3000000 INFO @ Sat, 06 Jul 2019 02:14:18: 3000000 INFO @ Sat, 06 Jul 2019 02:14:25: 4000000 INFO @ Sat, 06 Jul 2019 02:14:28: 4000000 INFO @ Sat, 06 Jul 2019 02:14:28: 4000000 INFO @ Sat, 06 Jul 2019 02:14:32: 5000000 INFO @ Sat, 06 Jul 2019 02:14:37: 5000000 INFO @ Sat, 06 Jul 2019 02:14:38: 5000000 INFO @ Sat, 06 Jul 2019 02:14:40: 6000000 INFO @ Sat, 06 Jul 2019 02:14:46: 6000000 INFO @ Sat, 06 Jul 2019 02:14:47: 7000000 INFO @ Sat, 06 Jul 2019 02:14:48: 6000000 INFO @ Sat, 06 Jul 2019 02:14:54: 8000000 INFO @ Sat, 06 Jul 2019 02:14:55: 7000000 INFO @ Sat, 06 Jul 2019 02:14:57: 7000000 INFO @ Sat, 06 Jul 2019 02:15:02: 9000000 INFO @ Sat, 06 Jul 2019 02:15:04: 8000000 INFO @ Sat, 06 Jul 2019 02:15:07: 8000000 INFO @ Sat, 06 Jul 2019 02:15:09: 10000000 INFO @ Sat, 06 Jul 2019 02:15:14: 9000000 INFO @ Sat, 06 Jul 2019 02:15:16: 9000000 INFO @ Sat, 06 Jul 2019 02:15:17: 11000000 INFO @ Sat, 06 Jul 2019 02:15:18: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:15:18: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:15:18: #1 total tags in treatment: 11043011 INFO @ Sat, 06 Jul 2019 02:15:18: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:15:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:15:18: #1 tags after filtering in treatment: 11043011 INFO @ Sat, 06 Jul 2019 02:15:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:15:18: #1 finished! INFO @ Sat, 06 Jul 2019 02:15:18: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:15:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:15:19: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:15:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:15:19: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:15:23: 10000000 INFO @ Sat, 06 Jul 2019 02:15:24: 10000000 INFO @ Sat, 06 Jul 2019 02:15:32: 11000000 INFO @ Sat, 06 Jul 2019 02:15:32: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:15:32: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:15:32: #1 total tags in treatment: 11043011 INFO @ Sat, 06 Jul 2019 02:15:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:15:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:15:32: 11000000 INFO @ Sat, 06 Jul 2019 02:15:32: #1 tags after filtering in treatment: 11043011 INFO @ Sat, 06 Jul 2019 02:15:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:15:32: #1 finished! INFO @ Sat, 06 Jul 2019 02:15:32: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:15:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:15:32: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:15:32: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:15:32: #1 total tags in treatment: 11043011 INFO @ Sat, 06 Jul 2019 02:15:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:15:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:15:33: #1 tags after filtering in treatment: 11043011 INFO @ Sat, 06 Jul 2019 02:15:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:15:33: #1 finished! INFO @ Sat, 06 Jul 2019 02:15:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:15:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:15:33: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:15:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:15:33: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:15:34: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:15:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:15:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.05_peaks.narrowPeakcut: : No such file or directory/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.10_peaks.narrowPeak : No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.20_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.10_model.r’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.10_*.xls’: No such file or directory rm: rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.10_peaks.narrowPeak’cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.05_model.r’: No such file or directory : No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.05_*.xls’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553737/SRX4553737.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。