Job ID = 2011046 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,992,864 reads read : 23,992,864 reads written : 23,992,864 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:05 23992864 reads; of these: 23992864 (100.00%) were unpaired; of these: 1505897 (6.28%) aligned 0 times 19853202 (82.75%) aligned exactly 1 time 2633765 (10.98%) aligned >1 times 93.72% overall alignment rate Time searching: 00:04:05 Overall time: 00:04:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11889682 / 22486967 = 0.5287 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:08:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:08:30: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:08:30: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:08:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:08:31: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:08:31: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:08:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:08:32: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:08:32: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:08:38: 1000000 INFO @ Sat, 06 Jul 2019 02:08:38: 1000000 INFO @ Sat, 06 Jul 2019 02:08:39: 1000000 INFO @ Sat, 06 Jul 2019 02:08:45: 2000000 INFO @ Sat, 06 Jul 2019 02:08:46: 2000000 INFO @ Sat, 06 Jul 2019 02:08:46: 2000000 INFO @ Sat, 06 Jul 2019 02:08:52: 3000000 INFO @ Sat, 06 Jul 2019 02:08:53: 3000000 INFO @ Sat, 06 Jul 2019 02:08:53: 3000000 INFO @ Sat, 06 Jul 2019 02:08:59: 4000000 INFO @ Sat, 06 Jul 2019 02:08:59: 4000000 INFO @ Sat, 06 Jul 2019 02:09:01: 4000000 INFO @ Sat, 06 Jul 2019 02:09:05: 5000000 INFO @ Sat, 06 Jul 2019 02:09:06: 5000000 INFO @ Sat, 06 Jul 2019 02:09:08: 5000000 INFO @ Sat, 06 Jul 2019 02:09:12: 6000000 INFO @ Sat, 06 Jul 2019 02:09:13: 6000000 INFO @ Sat, 06 Jul 2019 02:09:15: 6000000 INFO @ Sat, 06 Jul 2019 02:09:19: 7000000 INFO @ Sat, 06 Jul 2019 02:09:19: 7000000 INFO @ Sat, 06 Jul 2019 02:09:22: 7000000 INFO @ Sat, 06 Jul 2019 02:09:25: 8000000 INFO @ Sat, 06 Jul 2019 02:09:26: 8000000 INFO @ Sat, 06 Jul 2019 02:09:30: 8000000 INFO @ Sat, 06 Jul 2019 02:09:32: 9000000 INFO @ Sat, 06 Jul 2019 02:09:32: 9000000 INFO @ Sat, 06 Jul 2019 02:09:37: 9000000 INFO @ Sat, 06 Jul 2019 02:09:39: 10000000 INFO @ Sat, 06 Jul 2019 02:09:39: 10000000 INFO @ Sat, 06 Jul 2019 02:09:43: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:09:43: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:09:43: #1 total tags in treatment: 10597285 INFO @ Sat, 06 Jul 2019 02:09:43: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:09:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:09:43: #1 tags after filtering in treatment: 10597285 INFO @ Sat, 06 Jul 2019 02:09:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:09:43: #1 finished! INFO @ Sat, 06 Jul 2019 02:09:43: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:09:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:09:43: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:09:43: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:09:43: #1 total tags in treatment: 10597285 INFO @ Sat, 06 Jul 2019 02:09:43: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:09:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:09:43: #1 tags after filtering in treatment: 10597285 INFO @ Sat, 06 Jul 2019 02:09:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:09:43: #1 finished! INFO @ Sat, 06 Jul 2019 02:09:43: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:09:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:09:44: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:09:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:09:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:09:44: 10000000 INFO @ Sat, 06 Jul 2019 02:09:44: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:09:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:09:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:09:48: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:09:48: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:09:48: #1 total tags in treatment: 10597285 INFO @ Sat, 06 Jul 2019 02:09:48: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:09:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:09:49: #1 tags after filtering in treatment: 10597285 INFO @ Sat, 06 Jul 2019 02:09:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:09:49: #1 finished! INFO @ Sat, 06 Jul 2019 02:09:49: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:09:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:09:49: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:09:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:09:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553736/SRX4553736.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。