Job ID = 2011042 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,959,264 reads read : 23,959,264 reads written : 23,959,264 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:28 23959264 reads; of these: 23959264 (100.00%) were unpaired; of these: 1744159 (7.28%) aligned 0 times 19823809 (82.74%) aligned exactly 1 time 2391296 (9.98%) aligned >1 times 92.72% overall alignment rate Time searching: 00:04:28 Overall time: 00:04:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 11864237 / 22215105 = 0.5341 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:08:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:08:17: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:08:17: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:08:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:08:18: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:08:18: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:08:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:08:24: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:08:24: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:08:25: 1000000 INFO @ Sat, 06 Jul 2019 02:08:26: 1000000 INFO @ Sat, 06 Jul 2019 02:08:34: 2000000 INFO @ Sat, 06 Jul 2019 02:08:35: 2000000 INFO @ Sat, 06 Jul 2019 02:08:35: 1000000 INFO @ Sat, 06 Jul 2019 02:08:42: 3000000 INFO @ Sat, 06 Jul 2019 02:08:43: 3000000 INFO @ Sat, 06 Jul 2019 02:08:46: 2000000 INFO @ Sat, 06 Jul 2019 02:08:50: 4000000 INFO @ Sat, 06 Jul 2019 02:08:52: 4000000 INFO @ Sat, 06 Jul 2019 02:08:57: 3000000 INFO @ Sat, 06 Jul 2019 02:08:58: 5000000 INFO @ Sat, 06 Jul 2019 02:09:01: 5000000 INFO @ Sat, 06 Jul 2019 02:09:06: 6000000 INFO @ Sat, 06 Jul 2019 02:09:07: 4000000 INFO @ Sat, 06 Jul 2019 02:09:09: 6000000 INFO @ Sat, 06 Jul 2019 02:09:13: 7000000 INFO @ Sat, 06 Jul 2019 02:09:18: 5000000 INFO @ Sat, 06 Jul 2019 02:09:18: 7000000 INFO @ Sat, 06 Jul 2019 02:09:21: 8000000 INFO @ Sat, 06 Jul 2019 02:09:26: 8000000 INFO @ Sat, 06 Jul 2019 02:09:28: 6000000 INFO @ Sat, 06 Jul 2019 02:09:29: 9000000 INFO @ Sat, 06 Jul 2019 02:09:35: 9000000 INFO @ Sat, 06 Jul 2019 02:09:36: 10000000 INFO @ Sat, 06 Jul 2019 02:09:39: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:09:39: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:09:39: #1 total tags in treatment: 10350868 INFO @ Sat, 06 Jul 2019 02:09:39: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:09:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:09:39: #1 tags after filtering in treatment: 10350868 INFO @ Sat, 06 Jul 2019 02:09:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:09:39: #1 finished! INFO @ Sat, 06 Jul 2019 02:09:39: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:09:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:09:39: 7000000 INFO @ Sat, 06 Jul 2019 02:09:40: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:09:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:09:40: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:09:43: 10000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:09:46: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:09:46: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:09:46: #1 total tags in treatment: 10350868 INFO @ Sat, 06 Jul 2019 02:09:46: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:09:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:09:47: #1 tags after filtering in treatment: 10350868 INFO @ Sat, 06 Jul 2019 02:09:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:09:47: #1 finished! INFO @ Sat, 06 Jul 2019 02:09:47: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:09:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:09:47: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:09:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:09:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:09:50: 8000000 INFO @ Sat, 06 Jul 2019 02:10:00: 9000000 INFO @ Sat, 06 Jul 2019 02:10:11: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 02:10:15: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:10:15: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:10:15: #1 total tags in treatment: 10350868 INFO @ Sat, 06 Jul 2019 02:10:15: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:10:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:10:15: #1 tags after filtering in treatment: 10350868 INFO @ Sat, 06 Jul 2019 02:10:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:10:15: #1 finished! INFO @ Sat, 06 Jul 2019 02:10:15: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:10:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:10:16: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:10:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:10:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553733/SRX4553733.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。