Job ID = 2011041 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,402,321 reads read : 27,402,321 reads written : 27,402,321 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 27402321 reads; of these: 27402321 (100.00%) were unpaired; of these: 1876784 (6.85%) aligned 0 times 22588960 (82.43%) aligned exactly 1 time 2936577 (10.72%) aligned >1 times 93.15% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14556509 / 25525537 = 0.5703 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:11:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:11:57: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:11:57: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:11:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:11:58: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:11:58: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:11:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:11:59: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:11:59: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:12:06: 1000000 INFO @ Sat, 06 Jul 2019 02:12:08: 1000000 INFO @ Sat, 06 Jul 2019 02:12:09: 1000000 INFO @ Sat, 06 Jul 2019 02:12:15: 2000000 INFO @ Sat, 06 Jul 2019 02:12:16: 2000000 INFO @ Sat, 06 Jul 2019 02:12:18: 2000000 INFO @ Sat, 06 Jul 2019 02:12:23: 3000000 INFO @ Sat, 06 Jul 2019 02:12:24: 3000000 INFO @ Sat, 06 Jul 2019 02:12:27: 3000000 INFO @ Sat, 06 Jul 2019 02:12:30: 4000000 INFO @ Sat, 06 Jul 2019 02:12:33: 4000000 INFO @ Sat, 06 Jul 2019 02:12:36: 4000000 INFO @ Sat, 06 Jul 2019 02:12:37: 5000000 INFO @ Sat, 06 Jul 2019 02:12:42: 5000000 INFO @ Sat, 06 Jul 2019 02:12:44: 6000000 INFO @ Sat, 06 Jul 2019 02:12:45: 5000000 INFO @ Sat, 06 Jul 2019 02:12:51: 6000000 INFO @ Sat, 06 Jul 2019 02:12:51: 7000000 INFO @ Sat, 06 Jul 2019 02:12:53: 6000000 INFO @ Sat, 06 Jul 2019 02:12:58: 8000000 INFO @ Sat, 06 Jul 2019 02:12:59: 7000000 INFO @ Sat, 06 Jul 2019 02:13:02: 7000000 INFO @ Sat, 06 Jul 2019 02:13:05: 9000000 INFO @ Sat, 06 Jul 2019 02:13:08: 8000000 INFO @ Sat, 06 Jul 2019 02:13:11: 8000000 INFO @ Sat, 06 Jul 2019 02:13:12: 10000000 INFO @ Sat, 06 Jul 2019 02:13:17: 9000000 INFO @ Sat, 06 Jul 2019 02:13:19: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:13:19: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:13:19: #1 total tags in treatment: 10969028 INFO @ Sat, 06 Jul 2019 02:13:19: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:13:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:13:19: #1 tags after filtering in treatment: 10969028 INFO @ Sat, 06 Jul 2019 02:13:19: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:13:19: #1 finished! INFO @ Sat, 06 Jul 2019 02:13:19: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:13:19: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:13:19: 9000000 INFO @ Sat, 06 Jul 2019 02:13:20: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:13:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:13:20: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 02:13:25: 10000000 INFO @ Sat, 06 Jul 2019 02:13:28: 10000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:13:34: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:13:34: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:13:34: #1 total tags in treatment: 10969028 INFO @ Sat, 06 Jul 2019 02:13:34: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:13:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:13:34: #1 tags after filtering in treatment: 10969028 INFO @ Sat, 06 Jul 2019 02:13:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:13:34: #1 finished! INFO @ Sat, 06 Jul 2019 02:13:34: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:13:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:13:35: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:13:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:13:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:13:36: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:13:36: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:13:36: #1 total tags in treatment: 10969028 INFO @ Sat, 06 Jul 2019 02:13:36: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:13:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:13:37: #1 tags after filtering in treatment: 10969028 INFO @ Sat, 06 Jul 2019 02:13:37: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:13:37: #1 finished! INFO @ Sat, 06 Jul 2019 02:13:37: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:13:37: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:13:37: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:13:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:13:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553732/SRX4553732.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。