Job ID = 2011039 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,468,412 reads read : 24,468,412 reads written : 24,468,412 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:25 24468412 reads; of these: 24468412 (100.00%) were unpaired; of these: 1684380 (6.88%) aligned 0 times 20210321 (82.60%) aligned exactly 1 time 2573711 (10.52%) aligned >1 times 93.12% overall alignment rate Time searching: 00:04:25 Overall time: 00:04:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12437578 / 22784032 = 0.5459 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:09:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:09:34: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:09:34: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:09:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:09:35: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:09:35: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:09:44: 1000000 INFO @ Sat, 06 Jul 2019 02:09:44: 1000000 INFO @ Sat, 06 Jul 2019 02:09:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:09:46: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:09:46: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:09:53: 2000000 INFO @ Sat, 06 Jul 2019 02:09:54: 1000000 INFO @ Sat, 06 Jul 2019 02:09:54: 2000000 INFO @ Sat, 06 Jul 2019 02:10:02: 2000000 INFO @ Sat, 06 Jul 2019 02:10:02: 3000000 INFO @ Sat, 06 Jul 2019 02:10:04: 3000000 INFO @ Sat, 06 Jul 2019 02:10:10: 3000000 INFO @ Sat, 06 Jul 2019 02:10:11: 4000000 INFO @ Sat, 06 Jul 2019 02:10:14: 4000000 INFO @ Sat, 06 Jul 2019 02:10:17: 4000000 INFO @ Sat, 06 Jul 2019 02:10:20: 5000000 INFO @ Sat, 06 Jul 2019 02:10:23: 5000000 INFO @ Sat, 06 Jul 2019 02:10:25: 5000000 INFO @ Sat, 06 Jul 2019 02:10:28: 6000000 INFO @ Sat, 06 Jul 2019 02:10:32: 6000000 INFO @ Sat, 06 Jul 2019 02:10:33: 6000000 INFO @ Sat, 06 Jul 2019 02:10:37: 7000000 INFO @ Sat, 06 Jul 2019 02:10:41: 7000000 INFO @ Sat, 06 Jul 2019 02:10:41: 7000000 INFO @ Sat, 06 Jul 2019 02:10:46: 8000000 INFO @ Sat, 06 Jul 2019 02:10:49: 8000000 INFO @ Sat, 06 Jul 2019 02:10:50: 8000000 INFO @ Sat, 06 Jul 2019 02:10:55: 9000000 INFO @ Sat, 06 Jul 2019 02:10:56: 9000000 INFO @ Sat, 06 Jul 2019 02:10:59: 9000000 INFO @ Sat, 06 Jul 2019 02:11:04: 10000000 INFO @ Sat, 06 Jul 2019 02:11:04: 10000000 INFO @ Sat, 06 Jul 2019 02:11:07: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:11:07: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:11:07: #1 total tags in treatment: 10346454 INFO @ Sat, 06 Jul 2019 02:11:07: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:11:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:11:07: #1 tags after filtering in treatment: 10346454 INFO @ Sat, 06 Jul 2019 02:11:07: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:11:07: #1 finished! INFO @ Sat, 06 Jul 2019 02:11:07: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:11:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:11:07: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:11:07: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:11:07: #1 total tags in treatment: 10346454 INFO @ Sat, 06 Jul 2019 02:11:07: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:11:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:11:08: #1 tags after filtering in treatment: 10346454 INFO @ Sat, 06 Jul 2019 02:11:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:11:08: #1 finished! INFO @ Sat, 06 Jul 2019 02:11:08: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:11:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:11:08: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:11:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:11:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:11:08: 10000000 INFO @ Sat, 06 Jul 2019 02:11:08: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:11:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:11:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:11:11: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:11:11: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:11:11: #1 total tags in treatment: 10346454 INFO @ Sat, 06 Jul 2019 02:11:11: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:11:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:11:11: #1 tags after filtering in treatment: 10346454 INFO @ Sat, 06 Jul 2019 02:11:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:11:11: #1 finished! INFO @ Sat, 06 Jul 2019 02:11:11: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:11:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:11:12: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:11:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:11:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553731/SRX4553731.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。