Job ID = 2011022 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,656,136 reads read : 15,312,272 reads written : 7,656,136 reads 0-length : 7,656,136 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:22 7656136 reads; of these: 7656136 (100.00%) were unpaired; of these: 588888 (7.69%) aligned 0 times 6014538 (78.56%) aligned exactly 1 time 1052710 (13.75%) aligned >1 times 92.31% overall alignment rate Time searching: 00:01:22 Overall time: 00:01:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1751195 / 7067248 = 0.2478 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:45:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:39: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:39: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:40: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:40: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:41: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:41: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:47: 1000000 INFO @ Sat, 06 Jul 2019 01:45:47: 1000000 INFO @ Sat, 06 Jul 2019 01:45:47: 1000000 INFO @ Sat, 06 Jul 2019 01:45:53: 2000000 INFO @ Sat, 06 Jul 2019 01:45:54: 2000000 INFO @ Sat, 06 Jul 2019 01:45:56: 2000000 INFO @ Sat, 06 Jul 2019 01:46:00: 3000000 INFO @ Sat, 06 Jul 2019 01:46:01: 3000000 INFO @ Sat, 06 Jul 2019 01:46:05: 3000000 INFO @ Sat, 06 Jul 2019 01:46:07: 4000000 INFO @ Sat, 06 Jul 2019 01:46:08: 4000000 INFO @ Sat, 06 Jul 2019 01:46:13: 4000000 INFO @ Sat, 06 Jul 2019 01:46:13: 5000000 INFO @ Sat, 06 Jul 2019 01:46:14: 5000000 INFO @ Sat, 06 Jul 2019 01:46:15: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:46:15: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:46:15: #1 total tags in treatment: 5316053 INFO @ Sat, 06 Jul 2019 01:46:15: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:46:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:46:16: #1 tags after filtering in treatment: 5316053 INFO @ Sat, 06 Jul 2019 01:46:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:46:16: #1 finished! INFO @ Sat, 06 Jul 2019 01:46:16: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:46:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:46:16: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:46:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:46:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:46:17: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:46:17: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:46:17: #1 total tags in treatment: 5316053 INFO @ Sat, 06 Jul 2019 01:46:17: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:46:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:46:17: #1 tags after filtering in treatment: 5316053 INFO @ Sat, 06 Jul 2019 01:46:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:46:17: #1 finished! INFO @ Sat, 06 Jul 2019 01:46:17: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:46:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:46:17: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:46:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:46:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:46:22: 5000000 INFO @ Sat, 06 Jul 2019 01:46:24: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:46:24: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:46:24: #1 total tags in treatment: 5316053 INFO @ Sat, 06 Jul 2019 01:46:24: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:46:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:46:24: #1 tags after filtering in treatment: 5316053 INFO @ Sat, 06 Jul 2019 01:46:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:46:24: #1 finished! INFO @ Sat, 06 Jul 2019 01:46:24: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:46:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:46:25: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:46:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:46:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550102/SRX4550102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。