Job ID = 2011020 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,615,114 reads read : 19,230,228 reads written : 9,615,114 reads 0-length : 9,615,114 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:38 9615114 reads; of these: 9615114 (100.00%) were unpaired; of these: 812989 (8.46%) aligned 0 times 7491628 (77.92%) aligned exactly 1 time 1310497 (13.63%) aligned >1 times 91.54% overall alignment rate Time searching: 00:01:38 Overall time: 00:01:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2772335 / 8802125 = 0.3150 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:45:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:45: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:45: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:46: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:46: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:45:47: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:45:47: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:45:53: 1000000 INFO @ Sat, 06 Jul 2019 01:45:54: 1000000 INFO @ Sat, 06 Jul 2019 01:45:55: 1000000 INFO @ Sat, 06 Jul 2019 01:46:01: 2000000 INFO @ Sat, 06 Jul 2019 01:46:02: 2000000 INFO @ Sat, 06 Jul 2019 01:46:03: 2000000 INFO @ Sat, 06 Jul 2019 01:46:08: 3000000 INFO @ Sat, 06 Jul 2019 01:46:09: 3000000 INFO @ Sat, 06 Jul 2019 01:46:11: 3000000 INFO @ Sat, 06 Jul 2019 01:46:15: 4000000 INFO @ Sat, 06 Jul 2019 01:46:17: 4000000 INFO @ Sat, 06 Jul 2019 01:46:19: 4000000 INFO @ Sat, 06 Jul 2019 01:46:23: 5000000 INFO @ Sat, 06 Jul 2019 01:46:24: 5000000 INFO @ Sat, 06 Jul 2019 01:46:26: 5000000 INFO @ Sat, 06 Jul 2019 01:46:30: 6000000 INFO @ Sat, 06 Jul 2019 01:46:30: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:46:30: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:46:30: #1 total tags in treatment: 6029790 INFO @ Sat, 06 Jul 2019 01:46:30: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:46:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:46:30: #1 tags after filtering in treatment: 6029790 INFO @ Sat, 06 Jul 2019 01:46:30: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:46:30: #1 finished! INFO @ Sat, 06 Jul 2019 01:46:30: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:46:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:46:31: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:46:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:46:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:46:31: 6000000 INFO @ Sat, 06 Jul 2019 01:46:32: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:46:32: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:46:32: #1 total tags in treatment: 6029790 INFO @ Sat, 06 Jul 2019 01:46:32: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:46:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:46:32: #1 tags after filtering in treatment: 6029790 INFO @ Sat, 06 Jul 2019 01:46:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:46:32: #1 finished! INFO @ Sat, 06 Jul 2019 01:46:32: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:46:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:46:32: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:46:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:46:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:46:34: 6000000 INFO @ Sat, 06 Jul 2019 01:46:34: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:46:34: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:46:34: #1 total tags in treatment: 6029790 INFO @ Sat, 06 Jul 2019 01:46:34: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:46:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:46:34: #1 tags after filtering in treatment: 6029790 INFO @ Sat, 06 Jul 2019 01:46:34: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:46:34: #1 finished! INFO @ Sat, 06 Jul 2019 01:46:34: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:46:34: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:46:35: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:46:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:46:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4550100/SRX4550100.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。