Job ID = 2011005 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,320,488 reads read : 66,640,976 reads written : 33,320,488 reads 0-length : 33,320,488 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:22 33320488 reads; of these: 33320488 (100.00%) were unpaired; of these: 577012 (1.73%) aligned 0 times 28477163 (85.46%) aligned exactly 1 time 4266313 (12.80%) aligned >1 times 98.27% overall alignment rate Time searching: 00:10:22 Overall time: 00:10:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 16926810 / 32743476 = 0.5170 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:04:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:04:35: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:04:35: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:04:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:04:35: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:04:35: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:04:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:04:36: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:04:36: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:04:42: 1000000 INFO @ Sat, 06 Jul 2019 02:04:43: 1000000 INFO @ Sat, 06 Jul 2019 02:04:43: 1000000 INFO @ Sat, 06 Jul 2019 02:04:49: 2000000 INFO @ Sat, 06 Jul 2019 02:04:50: 2000000 INFO @ Sat, 06 Jul 2019 02:04:52: 2000000 INFO @ Sat, 06 Jul 2019 02:04:55: 3000000 INFO @ Sat, 06 Jul 2019 02:04:57: 3000000 INFO @ Sat, 06 Jul 2019 02:05:00: 3000000 INFO @ Sat, 06 Jul 2019 02:05:02: 4000000 INFO @ Sat, 06 Jul 2019 02:05:03: 4000000 INFO @ Sat, 06 Jul 2019 02:05:08: 4000000 INFO @ Sat, 06 Jul 2019 02:05:08: 5000000 INFO @ Sat, 06 Jul 2019 02:05:10: 5000000 INFO @ Sat, 06 Jul 2019 02:05:15: 6000000 INFO @ Sat, 06 Jul 2019 02:05:16: 5000000 INFO @ Sat, 06 Jul 2019 02:05:16: 6000000 INFO @ Sat, 06 Jul 2019 02:05:22: 7000000 INFO @ Sat, 06 Jul 2019 02:05:23: 7000000 INFO @ Sat, 06 Jul 2019 02:05:24: 6000000 INFO @ Sat, 06 Jul 2019 02:05:28: 8000000 INFO @ Sat, 06 Jul 2019 02:05:29: 8000000 INFO @ Sat, 06 Jul 2019 02:05:32: 7000000 INFO @ Sat, 06 Jul 2019 02:05:35: 9000000 INFO @ Sat, 06 Jul 2019 02:05:36: 9000000 INFO @ Sat, 06 Jul 2019 02:05:40: 8000000 INFO @ Sat, 06 Jul 2019 02:05:42: 10000000 INFO @ Sat, 06 Jul 2019 02:05:42: 10000000 INFO @ Sat, 06 Jul 2019 02:05:47: 9000000 INFO @ Sat, 06 Jul 2019 02:05:48: 11000000 INFO @ Sat, 06 Jul 2019 02:05:49: 11000000 INFO @ Sat, 06 Jul 2019 02:05:55: 12000000 INFO @ Sat, 06 Jul 2019 02:05:55: 10000000 INFO @ Sat, 06 Jul 2019 02:05:55: 12000000 INFO @ Sat, 06 Jul 2019 02:06:01: 13000000 INFO @ Sat, 06 Jul 2019 02:06:02: 13000000 INFO @ Sat, 06 Jul 2019 02:06:03: 11000000 INFO @ Sat, 06 Jul 2019 02:06:08: 14000000 INFO @ Sat, 06 Jul 2019 02:06:08: 14000000 INFO @ Sat, 06 Jul 2019 02:06:10: 12000000 INFO @ Sat, 06 Jul 2019 02:06:14: 15000000 INFO @ Sat, 06 Jul 2019 02:06:15: 15000000 INFO @ Sat, 06 Jul 2019 02:06:18: 13000000 INFO @ Sat, 06 Jul 2019 02:06:19: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:06:19: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:06:19: #1 total tags in treatment: 15816666 INFO @ Sat, 06 Jul 2019 02:06:19: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:06:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:06:20: #1 tags after filtering in treatment: 15816666 INFO @ Sat, 06 Jul 2019 02:06:20: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:06:20: #1 finished! INFO @ Sat, 06 Jul 2019 02:06:20: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:06:20: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:06:20: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:06:20: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:06:20: #1 total tags in treatment: 15816666 INFO @ Sat, 06 Jul 2019 02:06:20: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:06:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:06:21: #1 tags after filtering in treatment: 15816666 INFO @ Sat, 06 Jul 2019 02:06:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:06:21: #1 finished! INFO @ Sat, 06 Jul 2019 02:06:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:06:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:06:21: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:06:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:06:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:06:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:06:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:06:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:06:25: 14000000 INFO @ Sat, 06 Jul 2019 02:06:33: 15000000 INFO @ Sat, 06 Jul 2019 02:06:39: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:06:39: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:06:39: #1 total tags in treatment: 15816666 INFO @ Sat, 06 Jul 2019 02:06:39: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:06:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:06:39: #1 tags after filtering in treatment: 15816666 INFO @ Sat, 06 Jul 2019 02:06:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:06:39: #1 finished! INFO @ Sat, 06 Jul 2019 02:06:39: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:06:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:06:41: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:06:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:06:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409373/SRX4409373.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。