Job ID = 2011004 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,405,502 reads read : 66,811,004 reads written : 33,405,502 reads 0-length : 33,405,502 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:44 33405502 reads; of these: 33405502 (100.00%) were unpaired; of these: 1898723 (5.68%) aligned 0 times 27379951 (81.96%) aligned exactly 1 time 4126828 (12.35%) aligned >1 times 94.32% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 18301275 / 31506779 = 0.5809 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:58:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:58:41: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:58:41: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:58:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:58:42: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:58:42: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:58:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:58:43: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:58:43: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:58:49: 1000000 INFO @ Sat, 06 Jul 2019 01:58:49: 1000000 INFO @ Sat, 06 Jul 2019 01:58:51: 1000000 INFO @ Sat, 06 Jul 2019 01:58:56: 2000000 INFO @ Sat, 06 Jul 2019 01:58:57: 2000000 INFO @ Sat, 06 Jul 2019 01:58:59: 2000000 INFO @ Sat, 06 Jul 2019 01:59:03: 3000000 INFO @ Sat, 06 Jul 2019 01:59:04: 3000000 INFO @ Sat, 06 Jul 2019 01:59:06: 3000000 INFO @ Sat, 06 Jul 2019 01:59:10: 4000000 INFO @ Sat, 06 Jul 2019 01:59:12: 4000000 INFO @ Sat, 06 Jul 2019 01:59:14: 4000000 INFO @ Sat, 06 Jul 2019 01:59:17: 5000000 INFO @ Sat, 06 Jul 2019 01:59:20: 5000000 INFO @ Sat, 06 Jul 2019 01:59:22: 5000000 INFO @ Sat, 06 Jul 2019 01:59:24: 6000000 INFO @ Sat, 06 Jul 2019 01:59:28: 6000000 INFO @ Sat, 06 Jul 2019 01:59:29: 6000000 INFO @ Sat, 06 Jul 2019 01:59:31: 7000000 INFO @ Sat, 06 Jul 2019 01:59:35: 7000000 INFO @ Sat, 06 Jul 2019 01:59:37: 7000000 INFO @ Sat, 06 Jul 2019 01:59:38: 8000000 INFO @ Sat, 06 Jul 2019 01:59:42: 8000000 INFO @ Sat, 06 Jul 2019 01:59:44: 8000000 INFO @ Sat, 06 Jul 2019 01:59:44: 9000000 INFO @ Sat, 06 Jul 2019 01:59:50: 9000000 INFO @ Sat, 06 Jul 2019 01:59:51: 10000000 INFO @ Sat, 06 Jul 2019 01:59:52: 9000000 INFO @ Sat, 06 Jul 2019 01:59:57: 10000000 INFO @ Sat, 06 Jul 2019 01:59:58: 11000000 INFO @ Sat, 06 Jul 2019 01:59:59: 10000000 INFO @ Sat, 06 Jul 2019 02:00:05: 12000000 INFO @ Sat, 06 Jul 2019 02:00:05: 11000000 INFO @ Sat, 06 Jul 2019 02:00:07: 11000000 INFO @ Sat, 06 Jul 2019 02:00:12: 13000000 INFO @ Sat, 06 Jul 2019 02:00:12: 12000000 INFO @ Sat, 06 Jul 2019 02:00:13: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:00:13: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:00:13: #1 total tags in treatment: 13205504 INFO @ Sat, 06 Jul 2019 02:00:13: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:00:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:00:13: #1 tags after filtering in treatment: 13205504 INFO @ Sat, 06 Jul 2019 02:00:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:00:13: #1 finished! INFO @ Sat, 06 Jul 2019 02:00:13: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:00:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:00:14: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:00:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:00:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:00:14: 12000000 INFO @ Sat, 06 Jul 2019 02:00:20: 13000000 INFO @ Sat, 06 Jul 2019 02:00:21: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:00:21: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:00:21: #1 total tags in treatment: 13205504 INFO @ Sat, 06 Jul 2019 02:00:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:00:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:00:21: #1 tags after filtering in treatment: 13205504 INFO @ Sat, 06 Jul 2019 02:00:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:00:21: #1 finished! INFO @ Sat, 06 Jul 2019 02:00:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:00:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:00:22: 13000000 INFO @ Sat, 06 Jul 2019 02:00:23: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:00:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:00:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:00:23: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:00:23: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:00:23: #1 total tags in treatment: 13205504 INFO @ Sat, 06 Jul 2019 02:00:23: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:00:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:00:24: #1 tags after filtering in treatment: 13205504 INFO @ Sat, 06 Jul 2019 02:00:24: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:00:24: #1 finished! INFO @ Sat, 06 Jul 2019 02:00:24: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:00:24: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:00:25: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:00:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:00:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409372/SRX4409372.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。