Job ID = 2011002 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 33,094,013 reads read : 66,188,026 reads written : 33,094,013 reads 0-length : 33,094,013 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:25 33094013 reads; of these: 33094013 (100.00%) were unpaired; of these: 1712926 (5.18%) aligned 0 times 27479765 (83.04%) aligned exactly 1 time 3901322 (11.79%) aligned >1 times 94.82% overall alignment rate Time searching: 00:06:25 Overall time: 00:06:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 17967657 / 31381087 = 0.5726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:58:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:58:57: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:58:57: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:58:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:58:58: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:58:58: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:58:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:58:59: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:58:59: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:59:05: 1000000 INFO @ Sat, 06 Jul 2019 01:59:05: 1000000 INFO @ Sat, 06 Jul 2019 01:59:08: 1000000 INFO @ Sat, 06 Jul 2019 01:59:12: 2000000 INFO @ Sat, 06 Jul 2019 01:59:13: 2000000 INFO @ Sat, 06 Jul 2019 01:59:18: 2000000 INFO @ Sat, 06 Jul 2019 01:59:20: 3000000 INFO @ Sat, 06 Jul 2019 01:59:21: 3000000 INFO @ Sat, 06 Jul 2019 01:59:27: 4000000 INFO @ Sat, 06 Jul 2019 01:59:27: 3000000 INFO @ Sat, 06 Jul 2019 01:59:29: 4000000 INFO @ Sat, 06 Jul 2019 01:59:34: 5000000 INFO @ Sat, 06 Jul 2019 01:59:36: 4000000 INFO @ Sat, 06 Jul 2019 01:59:36: 5000000 INFO @ Sat, 06 Jul 2019 01:59:41: 6000000 INFO @ Sat, 06 Jul 2019 01:59:44: 6000000 INFO @ Sat, 06 Jul 2019 01:59:45: 5000000 INFO @ Sat, 06 Jul 2019 01:59:48: 7000000 INFO @ Sat, 06 Jul 2019 01:59:52: 7000000 INFO @ Sat, 06 Jul 2019 01:59:54: 6000000 INFO @ Sat, 06 Jul 2019 01:59:55: 8000000 INFO @ Sat, 06 Jul 2019 02:00:00: 8000000 INFO @ Sat, 06 Jul 2019 02:00:02: 9000000 INFO @ Sat, 06 Jul 2019 02:00:04: 7000000 INFO @ Sat, 06 Jul 2019 02:00:07: 9000000 INFO @ Sat, 06 Jul 2019 02:00:09: 10000000 INFO @ Sat, 06 Jul 2019 02:00:13: 8000000 INFO @ Sat, 06 Jul 2019 02:00:15: 10000000 INFO @ Sat, 06 Jul 2019 02:00:16: 11000000 INFO @ Sat, 06 Jul 2019 02:00:22: 9000000 INFO @ Sat, 06 Jul 2019 02:00:23: 11000000 INFO @ Sat, 06 Jul 2019 02:00:23: 12000000 INFO @ Sat, 06 Jul 2019 02:00:30: 13000000 INFO @ Sat, 06 Jul 2019 02:00:31: 12000000 INFO @ Sat, 06 Jul 2019 02:00:31: 10000000 INFO @ Sat, 06 Jul 2019 02:00:33: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:00:33: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:00:33: #1 total tags in treatment: 13413430 INFO @ Sat, 06 Jul 2019 02:00:33: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:00:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:00:33: #1 tags after filtering in treatment: 13413430 INFO @ Sat, 06 Jul 2019 02:00:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:00:33: #1 finished! INFO @ Sat, 06 Jul 2019 02:00:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:00:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:00:34: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:00:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:00:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:00:38: 13000000 INFO @ Sat, 06 Jul 2019 02:00:40: 11000000 INFO @ Sat, 06 Jul 2019 02:00:41: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:00:41: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:00:41: #1 total tags in treatment: 13413430 INFO @ Sat, 06 Jul 2019 02:00:41: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:00:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:00:41: #1 tags after filtering in treatment: 13413430 INFO @ Sat, 06 Jul 2019 02:00:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:00:41: #1 finished! INFO @ Sat, 06 Jul 2019 02:00:41: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:00:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:00:42: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:00:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:00:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:00:49: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 02:00:58: 13000000 INFO @ Sat, 06 Jul 2019 02:01:01: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:01:01: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:01:01: #1 total tags in treatment: 13413430 INFO @ Sat, 06 Jul 2019 02:01:01: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:01:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:01:01: #1 tags after filtering in treatment: 13413430 INFO @ Sat, 06 Jul 2019 02:01:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:01:01: #1 finished! INFO @ Sat, 06 Jul 2019 02:01:01: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:01:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:01:02: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:01:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:01:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409370/SRX4409370.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。