Job ID = 2010970 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,472,339 reads read : 16,944,678 reads written : 8,472,339 reads 0-length : 8,472,339 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:58 8472339 reads; of these: 8472339 (100.00%) were unpaired; of these: 1329121 (15.69%) aligned 0 times 5682680 (67.07%) aligned exactly 1 time 1460538 (17.24%) aligned >1 times 84.31% overall alignment rate Time searching: 00:01:58 Overall time: 00:01:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2273186 / 7143218 = 0.3182 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:36:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:36:14: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:36:14: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:36:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:36:15: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:36:15: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:36:22: 1000000 INFO @ Sat, 06 Jul 2019 01:36:23: 1000000 INFO @ Sat, 06 Jul 2019 01:36:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:36:28: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:36:28: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:36:30: 2000000 INFO @ Sat, 06 Jul 2019 01:36:31: 2000000 INFO @ Sat, 06 Jul 2019 01:36:36: 1000000 INFO @ Sat, 06 Jul 2019 01:36:38: 3000000 INFO @ Sat, 06 Jul 2019 01:36:39: 3000000 INFO @ Sat, 06 Jul 2019 01:36:44: 2000000 INFO @ Sat, 06 Jul 2019 01:36:45: 4000000 INFO @ Sat, 06 Jul 2019 01:36:46: 4000000 INFO @ Sat, 06 Jul 2019 01:36:52: #1 tag size is determined as 68 bps INFO @ Sat, 06 Jul 2019 01:36:52: #1 tag size = 68 INFO @ Sat, 06 Jul 2019 01:36:52: #1 total tags in treatment: 4870032 INFO @ Sat, 06 Jul 2019 01:36:52: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:36:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:36:52: #1 tags after filtering in treatment: 4870032 INFO @ Sat, 06 Jul 2019 01:36:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:36:52: #1 finished! INFO @ Sat, 06 Jul 2019 01:36:52: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:36:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:36:52: 3000000 INFO @ Sat, 06 Jul 2019 01:36:52: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:36:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:36:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:36:53: #1 tag size is determined as 68 bps INFO @ Sat, 06 Jul 2019 01:36:53: #1 tag size = 68 INFO @ Sat, 06 Jul 2019 01:36:53: #1 total tags in treatment: 4870032 INFO @ Sat, 06 Jul 2019 01:36:53: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:36:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:36:53: #1 tags after filtering in treatment: 4870032 INFO @ Sat, 06 Jul 2019 01:36:53: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:36:53: #1 finished! INFO @ Sat, 06 Jul 2019 01:36:53: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:36:53: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:36:53: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:36:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:36:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:36:59: 4000000 INFO @ Sat, 06 Jul 2019 01:37:06: #1 tag size is determined as 68 bps INFO @ Sat, 06 Jul 2019 01:37:06: #1 tag size = 68 INFO @ Sat, 06 Jul 2019 01:37:06: #1 total tags in treatment: 4870032 INFO @ Sat, 06 Jul 2019 01:37:06: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:37:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:37:06: #1 tags after filtering in treatment: 4870032 INFO @ Sat, 06 Jul 2019 01:37:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:37:06: #1 finished! INFO @ Sat, 06 Jul 2019 01:37:06: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:37:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:37:06: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:37:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:37:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342620/SRX4342620.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。