Job ID = 2010963 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,251,867 reads read : 20,503,734 reads written : 10,251,867 reads 0-length : 10,251,867 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:18 10251867 reads; of these: 10251867 (100.00%) were unpaired; of these: 955876 (9.32%) aligned 0 times 8017743 (78.21%) aligned exactly 1 time 1278248 (12.47%) aligned >1 times 90.68% overall alignment rate Time searching: 00:02:18 Overall time: 00:02:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2788623 / 9295991 = 0.3000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:35:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:35:29: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:35:29: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:35:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:35:30: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:35:30: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:35:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:35:31: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:35:31: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:35:38: 1000000 INFO @ Sat, 06 Jul 2019 01:35:39: 1000000 INFO @ Sat, 06 Jul 2019 01:35:41: 1000000 INFO @ Sat, 06 Jul 2019 01:35:47: 2000000 INFO @ Sat, 06 Jul 2019 01:35:48: 2000000 INFO @ Sat, 06 Jul 2019 01:35:50: 2000000 INFO @ Sat, 06 Jul 2019 01:35:56: 3000000 INFO @ Sat, 06 Jul 2019 01:35:56: 3000000 INFO @ Sat, 06 Jul 2019 01:35:59: 3000000 INFO @ Sat, 06 Jul 2019 01:36:04: 4000000 INFO @ Sat, 06 Jul 2019 01:36:05: 4000000 INFO @ Sat, 06 Jul 2019 01:36:08: 4000000 INFO @ Sat, 06 Jul 2019 01:36:13: 5000000 INFO @ Sat, 06 Jul 2019 01:36:14: 5000000 INFO @ Sat, 06 Jul 2019 01:36:18: 5000000 INFO @ Sat, 06 Jul 2019 01:36:22: 6000000 INFO @ Sat, 06 Jul 2019 01:36:22: 6000000 INFO @ Sat, 06 Jul 2019 01:36:26: #1 tag size is determined as 67 bps INFO @ Sat, 06 Jul 2019 01:36:26: #1 tag size = 67 INFO @ Sat, 06 Jul 2019 01:36:26: #1 total tags in treatment: 6507368 INFO @ Sat, 06 Jul 2019 01:36:26: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:36:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:36:26: #1 tags after filtering in treatment: 6507368 INFO @ Sat, 06 Jul 2019 01:36:26: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:36:26: #1 finished! INFO @ Sat, 06 Jul 2019 01:36:26: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:36:26: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:36:26: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:36:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:36:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:36:27: #1 tag size is determined as 67 bps INFO @ Sat, 06 Jul 2019 01:36:27: #1 tag size = 67 INFO @ Sat, 06 Jul 2019 01:36:27: #1 total tags in treatment: 6507368 INFO @ Sat, 06 Jul 2019 01:36:27: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:36:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:36:27: #1 tags after filtering in treatment: 6507368 INFO @ Sat, 06 Jul 2019 01:36:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:36:27: #1 finished! INFO @ Sat, 06 Jul 2019 01:36:27: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:36:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:36:27: 6000000 INFO @ Sat, 06 Jul 2019 01:36:27: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:36:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:36:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:36:31: #1 tag size is determined as 67 bps INFO @ Sat, 06 Jul 2019 01:36:31: #1 tag size = 67 INFO @ Sat, 06 Jul 2019 01:36:31: #1 total tags in treatment: 6507368 INFO @ Sat, 06 Jul 2019 01:36:31: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:36:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:36:32: #1 tags after filtering in treatment: 6507368 INFO @ Sat, 06 Jul 2019 01:36:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:36:32: #1 finished! INFO @ Sat, 06 Jul 2019 01:36:32: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:36:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:36:32: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:36:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:36:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342613/SRX4342613.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。