Job ID = 2010960 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,872,039 reads read : 17,744,078 reads written : 8,872,039 reads 0-length : 8,872,039 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:44 8872039 reads; of these: 8872039 (100.00%) were unpaired; of these: 1128428 (12.72%) aligned 0 times 6753003 (76.12%) aligned exactly 1 time 990608 (11.17%) aligned >1 times 87.28% overall alignment rate Time searching: 00:01:44 Overall time: 00:01:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1681881 / 7743611 = 0.2172 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:32:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:32:43: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:32:43: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:32:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:32:44: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:32:44: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:32:50: 1000000 INFO @ Sat, 06 Jul 2019 01:32:52: 1000000 INFO @ Sat, 06 Jul 2019 01:32:58: 2000000 INFO @ Sat, 06 Jul 2019 01:32:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:32:59: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:32:59: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:33:01: 2000000 INFO @ Sat, 06 Jul 2019 01:33:05: 3000000 INFO @ Sat, 06 Jul 2019 01:33:08: 1000000 INFO @ Sat, 06 Jul 2019 01:33:09: 3000000 INFO @ Sat, 06 Jul 2019 01:33:12: 4000000 INFO @ Sat, 06 Jul 2019 01:33:17: 4000000 INFO @ Sat, 06 Jul 2019 01:33:17: 2000000 INFO @ Sat, 06 Jul 2019 01:33:20: 5000000 INFO @ Sat, 06 Jul 2019 01:33:25: 5000000 INFO @ Sat, 06 Jul 2019 01:33:26: 3000000 INFO @ Sat, 06 Jul 2019 01:33:27: 6000000 INFO @ Sat, 06 Jul 2019 01:33:27: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:33:27: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:33:27: #1 total tags in treatment: 6061730 INFO @ Sat, 06 Jul 2019 01:33:27: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:33:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:33:27: #1 tags after filtering in treatment: 6061730 INFO @ Sat, 06 Jul 2019 01:33:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:33:27: #1 finished! INFO @ Sat, 06 Jul 2019 01:33:27: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:33:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:33:28: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:33:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:33:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:33:33: 6000000 INFO @ Sat, 06 Jul 2019 01:33:33: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:33:33: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:33:33: #1 total tags in treatment: 6061730 INFO @ Sat, 06 Jul 2019 01:33:33: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:33:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:33:33: #1 tags after filtering in treatment: 6061730 INFO @ Sat, 06 Jul 2019 01:33:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:33:33: #1 finished! INFO @ Sat, 06 Jul 2019 01:33:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:33:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:33:34: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:33:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:33:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:33:34: 4000000 INFO @ Sat, 06 Jul 2019 01:33:42: 5000000 INFO @ Sat, 06 Jul 2019 01:33:50: 6000000 INFO @ Sat, 06 Jul 2019 01:33:51: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:33:51: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:33:51: #1 total tags in treatment: 6061730 INFO @ Sat, 06 Jul 2019 01:33:51: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:33:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:33:51: #1 tags after filtering in treatment: 6061730 INFO @ Sat, 06 Jul 2019 01:33:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:33:51: #1 finished! INFO @ Sat, 06 Jul 2019 01:33:51: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:33:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:33:51: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:33:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:33:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342610/SRX4342610.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。