Job ID = 2010943 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,405,326 reads read : 26,810,652 reads written : 13,405,326 reads 0-length : 13,405,326 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:58 13405326 reads; of these: 13405326 (100.00%) were unpaired; of these: 1824021 (13.61%) aligned 0 times 10515889 (78.45%) aligned exactly 1 time 1065416 (7.95%) aligned >1 times 86.39% overall alignment rate Time searching: 00:02:58 Overall time: 00:02:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3321119 / 11581305 = 0.2868 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:31:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:31:46: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:31:46: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:31:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:31:47: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:31:47: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:31:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:31:48: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:31:48: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:31:54: 1000000 INFO @ Sat, 06 Jul 2019 01:31:56: 1000000 INFO @ Sat, 06 Jul 2019 01:31:56: 1000000 INFO @ Sat, 06 Jul 2019 01:32:01: 2000000 INFO @ Sat, 06 Jul 2019 01:32:05: 2000000 INFO @ Sat, 06 Jul 2019 01:32:07: 2000000 INFO @ Sat, 06 Jul 2019 01:32:08: 3000000 INFO @ Sat, 06 Jul 2019 01:32:13: 3000000 INFO @ Sat, 06 Jul 2019 01:32:15: 4000000 INFO @ Sat, 06 Jul 2019 01:32:17: 3000000 INFO @ Sat, 06 Jul 2019 01:32:21: 4000000 INFO @ Sat, 06 Jul 2019 01:32:22: 5000000 INFO @ Sat, 06 Jul 2019 01:32:27: 4000000 INFO @ Sat, 06 Jul 2019 01:32:28: 5000000 INFO @ Sat, 06 Jul 2019 01:32:30: 6000000 INFO @ Sat, 06 Jul 2019 01:32:36: 5000000 INFO @ Sat, 06 Jul 2019 01:32:36: 6000000 INFO @ Sat, 06 Jul 2019 01:32:37: 7000000 INFO @ Sat, 06 Jul 2019 01:32:44: 6000000 INFO @ Sat, 06 Jul 2019 01:32:44: 8000000 INFO @ Sat, 06 Jul 2019 01:32:44: 7000000 INFO @ Sat, 06 Jul 2019 01:32:46: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:32:46: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:32:46: #1 total tags in treatment: 8260186 INFO @ Sat, 06 Jul 2019 01:32:46: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:32:46: #1 tags after filtering in treatment: 8260186 INFO @ Sat, 06 Jul 2019 01:32:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:32:46: #1 finished! INFO @ Sat, 06 Jul 2019 01:32:46: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:32:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:32:46: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:32:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:32:46: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:32:51: 7000000 INFO @ Sat, 06 Jul 2019 01:32:52: 8000000 INFO @ Sat, 06 Jul 2019 01:32:54: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:32:54: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:32:54: #1 total tags in treatment: 8260186 INFO @ Sat, 06 Jul 2019 01:32:54: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:32:54: #1 tags after filtering in treatment: 8260186 INFO @ Sat, 06 Jul 2019 01:32:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:32:54: #1 finished! INFO @ Sat, 06 Jul 2019 01:32:54: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:32:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:32:54: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:32:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:32:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.05_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:32:58: 8000000 INFO @ Sat, 06 Jul 2019 01:32:59: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:32:59: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:32:59: #1 total tags in treatment: 8260186 INFO @ Sat, 06 Jul 2019 01:32:59: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:33:00: #1 tags after filtering in treatment: 8260186 INFO @ Sat, 06 Jul 2019 01:33:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:33:00: #1 finished! INFO @ Sat, 06 Jul 2019 01:33:00: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:33:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:33:00: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:33:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:33:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342595/SRX4342595.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。