Job ID = 2010940 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,588,149 reads read : 25,176,298 reads written : 12,588,149 reads 0-length : 12,588,149 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:50 12588149 reads; of these: 12588149 (100.00%) were unpaired; of these: 1253393 (9.96%) aligned 0 times 10309707 (81.90%) aligned exactly 1 time 1025049 (8.14%) aligned >1 times 90.04% overall alignment rate Time searching: 00:02:50 Overall time: 00:02:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3284182 / 11334756 = 0.2897 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:29:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:29:39: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:29:39: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:29:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:29:40: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:29:40: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:29:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:29:40: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:29:40: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:29:47: 1000000 INFO @ Sat, 06 Jul 2019 01:29:48: 1000000 INFO @ Sat, 06 Jul 2019 01:29:50: 1000000 INFO @ Sat, 06 Jul 2019 01:29:56: 2000000 INFO @ Sat, 06 Jul 2019 01:29:57: 2000000 INFO @ Sat, 06 Jul 2019 01:29:59: 2000000 INFO @ Sat, 06 Jul 2019 01:30:04: 3000000 INFO @ Sat, 06 Jul 2019 01:30:05: 3000000 INFO @ Sat, 06 Jul 2019 01:30:07: 3000000 INFO @ Sat, 06 Jul 2019 01:30:12: 4000000 INFO @ Sat, 06 Jul 2019 01:30:13: 4000000 INFO @ Sat, 06 Jul 2019 01:30:15: 4000000 INFO @ Sat, 06 Jul 2019 01:30:20: 5000000 INFO @ Sat, 06 Jul 2019 01:30:21: 5000000 INFO @ Sat, 06 Jul 2019 01:30:23: 5000000 INFO @ Sat, 06 Jul 2019 01:30:28: 6000000 INFO @ Sat, 06 Jul 2019 01:30:29: 6000000 INFO @ Sat, 06 Jul 2019 01:30:31: 6000000 INFO @ Sat, 06 Jul 2019 01:30:36: 7000000 INFO @ Sat, 06 Jul 2019 01:30:37: 7000000 INFO @ Sat, 06 Jul 2019 01:30:39: 7000000 INFO @ Sat, 06 Jul 2019 01:30:43: 8000000 INFO @ Sat, 06 Jul 2019 01:30:44: #1 tag size is determined as 67 bps INFO @ Sat, 06 Jul 2019 01:30:44: #1 tag size = 67 INFO @ Sat, 06 Jul 2019 01:30:44: #1 total tags in treatment: 8050574 INFO @ Sat, 06 Jul 2019 01:30:44: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:30:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:30:44: #1 tags after filtering in treatment: 8050574 INFO @ Sat, 06 Jul 2019 01:30:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:30:44: #1 finished! INFO @ Sat, 06 Jul 2019 01:30:44: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:30:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:30:44: 8000000 INFO @ Sat, 06 Jul 2019 01:30:45: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:30:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:30:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 06 Jul 2019 01:30:45: #1 tag size is determined as 67 bps INFO @ Sat, 06 Jul 2019 01:30:45: #1 tag size = 67 INFO @ Sat, 06 Jul 2019 01:30:45: #1 total tags in treatment: 8050574 INFO @ Sat, 06 Jul 2019 01:30:45: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:30:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:30:45: #1 tags after filtering in treatment: 8050574 INFO @ Sat, 06 Jul 2019 01:30:45: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:30:45: #1 finished! INFO @ Sat, 06 Jul 2019 01:30:45: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:30:45: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:30:46: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:30:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:30:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:30:47: 8000000 INFO @ Sat, 06 Jul 2019 01:30:47: #1 tag size is determined as 67 bps INFO @ Sat, 06 Jul 2019 01:30:47: #1 tag size = 67 INFO @ Sat, 06 Jul 2019 01:30:47: #1 total tags in treatment: 8050574 INFO @ Sat, 06 Jul 2019 01:30:47: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:30:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:30:47: #1 tags after filtering in treatment: 8050574 INFO @ Sat, 06 Jul 2019 01:30:47: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:30:47: #1 finished! INFO @ Sat, 06 Jul 2019 01:30:47: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:30:47: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:30:48: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:30:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:30:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342593/SRX4342593.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。