Job ID = 11193170 sra ファイルのダウンロード中... Completed: 64271K bytes transferred in 7 seconds (71921K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 4550490 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4337762/SRR7467841.sra Written 4550490 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4337762/SRR7467841.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:36 4550490 reads; of these: 4550490 (100.00%) were unpaired; of these: 1770963 (38.92%) aligned 0 times 2278227 (50.07%) aligned exactly 1 time 501300 (11.02%) aligned >1 times 61.08% overall alignment rate Time searching: 00:00:36 Overall time: 00:00:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1422367 / 2779527 = 0.5117 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 11:05:26: # Command line: callpeak -t SRX4337762.bam -f BAM -g 12100000 -n SRX4337762.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4337762.20 # format = BAM # ChIP-seq file = ['SRX4337762.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:05:26: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:05:26: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:05:26: # Command line: callpeak -t SRX4337762.bam -f BAM -g 12100000 -n SRX4337762.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4337762.05 # format = BAM # ChIP-seq file = ['SRX4337762.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:05:26: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:05:26: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:05:26: # Command line: callpeak -t SRX4337762.bam -f BAM -g 12100000 -n SRX4337762.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4337762.10 # format = BAM # ChIP-seq file = ['SRX4337762.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:05:26: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:05:26: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:05:33: 1000000 INFO @ Sat, 15 Sep 2018 11:05:33: 1000000 INFO @ Sat, 15 Sep 2018 11:05:33: 1000000 INFO @ Sat, 15 Sep 2018 11:05:35: #1 tag size is determined as 51 bps INFO @ Sat, 15 Sep 2018 11:05:35: #1 tag size = 51 INFO @ Sat, 15 Sep 2018 11:05:35: #1 total tags in treatment: 1357160 INFO @ Sat, 15 Sep 2018 11:05:35: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:05:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:05:35: #1 tags after filtering in treatment: 1357160 INFO @ Sat, 15 Sep 2018 11:05:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:05:35: #1 finished! INFO @ Sat, 15 Sep 2018 11:05:35: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:05:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:05:35: #1 tag size is determined as 51 bps INFO @ Sat, 15 Sep 2018 11:05:35: #1 tag size = 51 INFO @ Sat, 15 Sep 2018 11:05:35: #1 total tags in treatment: 1357160 INFO @ Sat, 15 Sep 2018 11:05:35: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:05:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:05:35: #1 tags after filtering in treatment: 1357160 INFO @ Sat, 15 Sep 2018 11:05:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:05:35: #1 finished! INFO @ Sat, 15 Sep 2018 11:05:35: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:05:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:05:35: #1 tag size is determined as 51 bps INFO @ Sat, 15 Sep 2018 11:05:35: #1 tag size = 51 INFO @ Sat, 15 Sep 2018 11:05:35: #1 total tags in treatment: 1357160 INFO @ Sat, 15 Sep 2018 11:05:35: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:05:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:05:35: #2 number of paired peaks: 120 WARNING @ Sat, 15 Sep 2018 11:05:35: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Sat, 15 Sep 2018 11:05:35: start model_add_line... INFO @ Sat, 15 Sep 2018 11:05:35: #1 tags after filtering in treatment: 1357160 INFO @ Sat, 15 Sep 2018 11:05:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:05:35: #1 finished! INFO @ Sat, 15 Sep 2018 11:05:35: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:05:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:05:35: start X-correlation... INFO @ Sat, 15 Sep 2018 11:05:36: end of X-cor INFO @ Sat, 15 Sep 2018 11:05:36: #2 finished! INFO @ Sat, 15 Sep 2018 11:05:36: #2 predicted fragment length is 224 bps INFO @ Sat, 15 Sep 2018 11:05:36: #2 alternative fragment length(s) may be 4,224 bps INFO @ Sat, 15 Sep 2018 11:05:36: #2.2 Generate R script for model : SRX4337762.20_model.r INFO @ Sat, 15 Sep 2018 11:05:36: #3 Call peaks... INFO @ Sat, 15 Sep 2018 11:05:36: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 11:05:36: #2 number of paired peaks: 120 WARNING @ Sat, 15 Sep 2018 11:05:36: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Sat, 15 Sep 2018 11:05:36: start model_add_line... INFO @ Sat, 15 Sep 2018 11:05:36: start X-correlation... INFO @ Sat, 15 Sep 2018 11:05:36: end of X-cor INFO @ Sat, 15 Sep 2018 11:05:36: #2 finished! INFO @ Sat, 15 Sep 2018 11:05:36: #2 predicted fragment length is 224 bps INFO @ Sat, 15 Sep 2018 11:05:36: #2 alternative fragment length(s) may be 4,224 bps INFO @ Sat, 15 Sep 2018 11:05:36: #2.2 Generate R script for model : SRX4337762.05_model.r INFO @ Sat, 15 Sep 2018 11:05:36: #3 Call peaks... INFO @ Sat, 15 Sep 2018 11:05:36: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 11:05:36: #2 number of paired peaks: 120 WARNING @ Sat, 15 Sep 2018 11:05:36: Fewer paired peaks (120) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 120 pairs to build model! INFO @ Sat, 15 Sep 2018 11:05:36: start model_add_line... INFO @ Sat, 15 Sep 2018 11:05:36: start X-correlation... INFO @ Sat, 15 Sep 2018 11:05:36: end of X-cor INFO @ Sat, 15 Sep 2018 11:05:36: #2 finished! INFO @ Sat, 15 Sep 2018 11:05:36: #2 predicted fragment length is 224 bps INFO @ Sat, 15 Sep 2018 11:05:36: #2 alternative fragment length(s) may be 4,224 bps INFO @ Sat, 15 Sep 2018 11:05:36: #2.2 Generate R script for model : SRX4337762.10_model.r INFO @ Sat, 15 Sep 2018 11:05:36: #3 Call peaks... INFO @ Sat, 15 Sep 2018 11:05:36: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 11:05:40: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 11:05:40: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 11:05:40: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write output xls file... SRX4337762.20_peaks.xls INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write peak in narrowPeak format file... SRX4337762.20_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write summits bed file... SRX4337762.20_summits.bed INFO @ Sat, 15 Sep 2018 11:05:41: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (210 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write output xls file... SRX4337762.05_peaks.xls INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write peak in narrowPeak format file... SRX4337762.05_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write summits bed file... SRX4337762.05_summits.bed INFO @ Sat, 15 Sep 2018 11:05:41: Done! INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write output xls file... SRX4337762.10_peaks.xls INFO @ Sat, 15 Sep 2018 11:05:41: #4 Write peak in narrowPeak format file... SRX4337762.10_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 11:05:42: #4 Write summits bed file... SRX4337762.10_summits.bed INFO @ Sat, 15 Sep 2018 11:05:42: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (707 records, 4 fields): 3 millis CompletedMACS2peakCalling pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (393 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。