Job ID = 2010932 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,842,184 reads read : 16,842,184 reads written : 16,842,184 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1118266.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:00 16842184 reads; of these: 16842184 (100.00%) were unpaired; of these: 500478 (2.97%) aligned 0 times 14457833 (85.84%) aligned exactly 1 time 1883873 (11.19%) aligned >1 times 97.03% overall alignment rate Time searching: 00:05:00 Overall time: 00:05:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8329241 / 16341706 = 0.5097 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:31:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:31:01: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:31:01: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:31:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:31:02: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:31:02: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:31:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:31:03: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:31:03: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:31:08: 1000000 INFO @ Sat, 06 Jul 2019 01:31:09: 1000000 INFO @ Sat, 06 Jul 2019 01:31:11: 1000000 INFO @ Sat, 06 Jul 2019 01:31:15: 2000000 INFO @ Sat, 06 Jul 2019 01:31:17: 2000000 INFO @ Sat, 06 Jul 2019 01:31:19: 2000000 INFO @ Sat, 06 Jul 2019 01:31:23: 3000000 INFO @ Sat, 06 Jul 2019 01:31:24: 3000000 INFO @ Sat, 06 Jul 2019 01:31:27: 3000000 INFO @ Sat, 06 Jul 2019 01:31:30: 4000000 INFO @ Sat, 06 Jul 2019 01:31:31: 4000000 INFO @ Sat, 06 Jul 2019 01:31:34: 4000000 INFO @ Sat, 06 Jul 2019 01:31:37: 5000000 INFO @ Sat, 06 Jul 2019 01:31:38: 5000000 INFO @ Sat, 06 Jul 2019 01:31:42: 5000000 INFO @ Sat, 06 Jul 2019 01:31:44: 6000000 INFO @ Sat, 06 Jul 2019 01:31:46: 6000000 INFO @ Sat, 06 Jul 2019 01:31:49: 6000000 INFO @ Sat, 06 Jul 2019 01:31:51: 7000000 INFO @ Sat, 06 Jul 2019 01:31:53: 7000000 INFO @ Sat, 06 Jul 2019 01:31:57: 7000000 INFO @ Sat, 06 Jul 2019 01:31:58: 8000000 INFO @ Sat, 06 Jul 2019 01:31:58: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 01:31:58: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 01:31:58: #1 total tags in treatment: 8012465 INFO @ Sat, 06 Jul 2019 01:31:58: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:31:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:31:59: #1 tags after filtering in treatment: 8012465 INFO @ Sat, 06 Jul 2019 01:31:59: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:31:59: #1 finished! INFO @ Sat, 06 Jul 2019 01:31:59: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:31:59: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:31:59: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:31:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:31:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:32:00: 8000000 INFO @ Sat, 06 Jul 2019 01:32:00: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 01:32:00: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 01:32:00: #1 total tags in treatment: 8012465 INFO @ Sat, 06 Jul 2019 01:32:00: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:32:00: #1 tags after filtering in treatment: 8012465 INFO @ Sat, 06 Jul 2019 01:32:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:32:00: #1 finished! INFO @ Sat, 06 Jul 2019 01:32:00: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:32:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:32:01: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:32:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:32:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:32:04: 8000000 INFO @ Sat, 06 Jul 2019 01:32:04: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 01:32:04: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 01:32:04: #1 total tags in treatment: 8012465 INFO @ Sat, 06 Jul 2019 01:32:04: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:32:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:32:04: #1 tags after filtering in treatment: 8012465 INFO @ Sat, 06 Jul 2019 01:32:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:32:04: #1 finished! INFO @ Sat, 06 Jul 2019 01:32:04: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:32:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:32:05: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:32:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:32:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433138/SRX433138.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。