Job ID = 2010927 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,837,965 reads read : 17,837,965 reads written : 17,837,965 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1118262.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:16 17837965 reads; of these: 17837965 (100.00%) were unpaired; of these: 594898 (3.34%) aligned 0 times 14687514 (82.34%) aligned exactly 1 time 2555553 (14.33%) aligned >1 times 96.66% overall alignment rate Time searching: 00:05:16 Overall time: 00:05:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 11318806 / 17243067 = 0.6564 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:29:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:29:58: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:29:58: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:29:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:29:59: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:29:59: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:30:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:30:25: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:30:25: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:30:32: 1000000 INFO @ Sat, 06 Jul 2019 01:30:32: 1000000 INFO @ Sat, 06 Jul 2019 01:30:33: 1000000 INFO @ Sat, 06 Jul 2019 01:30:42: 2000000 INFO @ Sat, 06 Jul 2019 01:30:42: 2000000 INFO @ Sat, 06 Jul 2019 01:30:42: 2000000 INFO @ Sat, 06 Jul 2019 01:30:50: 3000000 INFO @ Sat, 06 Jul 2019 01:30:52: 3000000 INFO @ Sat, 06 Jul 2019 01:30:52: 3000000 INFO @ Sat, 06 Jul 2019 01:30:58: 4000000 INFO @ Sat, 06 Jul 2019 01:31:01: 4000000 INFO @ Sat, 06 Jul 2019 01:31:01: 4000000 INFO @ Sat, 06 Jul 2019 01:31:06: 5000000 INFO @ Sat, 06 Jul 2019 01:31:10: 5000000 INFO @ Sat, 06 Jul 2019 01:31:10: 5000000 INFO @ Sat, 06 Jul 2019 01:31:13: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 01:31:13: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 01:31:13: #1 total tags in treatment: 5924261 INFO @ Sat, 06 Jul 2019 01:31:13: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:31:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:31:13: #1 tags after filtering in treatment: 5924261 INFO @ Sat, 06 Jul 2019 01:31:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:31:13: #1 finished! INFO @ Sat, 06 Jul 2019 01:31:13: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:31:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:31:13: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:31:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:31:13: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:31:18: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 01:31:18: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 01:31:18: #1 total tags in treatment: 5924261 INFO @ Sat, 06 Jul 2019 01:31:18: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:31:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:31:18: #1 tag size is determined as 101 bps INFO @ Sat, 06 Jul 2019 01:31:18: #1 tag size = 101 INFO @ Sat, 06 Jul 2019 01:31:18: #1 total tags in treatment: 5924261 INFO @ Sat, 06 Jul 2019 01:31:18: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:31:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:31:18: #1 tags after filtering in treatment: 5924261 INFO @ Sat, 06 Jul 2019 01:31:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:31:18: #1 finished! INFO @ Sat, 06 Jul 2019 01:31:18: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:31:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:31:18: #1 tags after filtering in treatment: 5924261 INFO @ Sat, 06 Jul 2019 01:31:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:31:18: #1 finished! INFO @ Sat, 06 Jul 2019 01:31:18: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:31:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:31:18: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:31:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:31:18: Process for pairing-model is terminated! INFO @ Sat, 06 Jul 2019 01:31:18: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:31:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:31:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.10_peaks.narrowPeakcut: : No such file or directory/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.05_peaks.narrowPeak : No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX433134/SRX433134.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。