Job ID = 2640918 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,341,087 reads read : 5,341,087 reads written : 5,341,087 spots read : 5,819,206 reads read : 5,819,206 reads written : 5,819,206 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:01 11160293 reads; of these: 11160293 (100.00%) were unpaired; of these: 660317 (5.92%) aligned 0 times 7990070 (71.59%) aligned exactly 1 time 2509906 (22.49%) aligned >1 times 94.08% overall alignment rate Time searching: 00:02:01 Overall time: 00:02:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4182495 / 10499976 = 0.3983 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:03:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:03:00: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:03:00: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:03:06: 1000000 INFO @ Sat, 24 Aug 2019 20:03:13: 2000000 INFO @ Sat, 24 Aug 2019 20:03:19: 3000000 INFO @ Sat, 24 Aug 2019 20:03:25: 4000000 INFO @ Sat, 24 Aug 2019 20:03:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:03:30: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:03:30: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:03:31: 5000000 INFO @ Sat, 24 Aug 2019 20:03:37: 1000000 INFO @ Sat, 24 Aug 2019 20:03:37: 6000000 INFO @ Sat, 24 Aug 2019 20:03:39: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:03:39: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:03:39: #1 total tags in treatment: 6317481 INFO @ Sat, 24 Aug 2019 20:03:39: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:03:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:03:39: #1 tags after filtering in treatment: 6317481 INFO @ Sat, 24 Aug 2019 20:03:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:03:39: #1 finished! INFO @ Sat, 24 Aug 2019 20:03:39: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:03:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:03:40: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:03:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:03:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:03:44: 2000000 INFO @ Sat, 24 Aug 2019 20:03:51: 3000000 INFO @ Sat, 24 Aug 2019 20:03:57: 4000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:04:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:04:00: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:04:00: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:04:04: 5000000 INFO @ Sat, 24 Aug 2019 20:04:07: 1000000 INFO @ Sat, 24 Aug 2019 20:04:10: 6000000 INFO @ Sat, 24 Aug 2019 20:04:13: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:04:13: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:04:13: #1 total tags in treatment: 6317481 INFO @ Sat, 24 Aug 2019 20:04:13: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:04:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:04:13: #1 tags after filtering in treatment: 6317481 INFO @ Sat, 24 Aug 2019 20:04:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:04:13: #1 finished! INFO @ Sat, 24 Aug 2019 20:04:13: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:04:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:04:13: 2000000 INFO @ Sat, 24 Aug 2019 20:04:13: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:04:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:04:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:04:19: 3000000 INFO @ Sat, 24 Aug 2019 20:04:25: 4000000 INFO @ Sat, 24 Aug 2019 20:04:31: 5000000 INFO @ Sat, 24 Aug 2019 20:04:37: 6000000 INFO @ Sat, 24 Aug 2019 20:04:39: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:04:39: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:04:39: #1 total tags in treatment: 6317481 INFO @ Sat, 24 Aug 2019 20:04:39: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:04:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:04:39: #1 tags after filtering in treatment: 6317481 INFO @ Sat, 24 Aug 2019 20:04:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:04:39: #1 finished! INFO @ Sat, 24 Aug 2019 20:04:39: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:04:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:04:40: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:04:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:04:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292282/SRX4292282.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。