Job ID = 2640913 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,578,977 reads read : 7,578,977 reads written : 7,578,977 spots read : 8,942,707 reads read : 8,942,707 reads written : 8,942,707 spots read : 8,789,537 reads read : 8,789,537 reads written : 8,789,537 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:19 25311221 reads; of these: 25311221 (100.00%) were unpaired; of these: 1919038 (7.58%) aligned 0 times 17874834 (70.62%) aligned exactly 1 time 5517349 (21.80%) aligned >1 times 92.42% overall alignment rate Time searching: 00:04:19 Overall time: 00:04:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12142388 / 23392183 = 0.5191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:16:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:16:54: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:16:54: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:17:04: 1000000 INFO @ Sat, 24 Aug 2019 20:17:12: 2000000 INFO @ Sat, 24 Aug 2019 20:17:21: 3000000 INFO @ Sat, 24 Aug 2019 20:17:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:17:24: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:17:24: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:17:29: 4000000 INFO @ Sat, 24 Aug 2019 20:17:34: 1000000 INFO @ Sat, 24 Aug 2019 20:17:39: 5000000 INFO @ Sat, 24 Aug 2019 20:17:43: 2000000 INFO @ Sat, 24 Aug 2019 20:17:48: 6000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:17:53: 3000000 INFO @ Sat, 24 Aug 2019 20:17:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:17:54: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:17:54: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:17:58: 7000000 INFO @ Sat, 24 Aug 2019 20:18:02: 4000000 INFO @ Sat, 24 Aug 2019 20:18:04: 1000000 INFO @ Sat, 24 Aug 2019 20:18:08: 8000000 INFO @ Sat, 24 Aug 2019 20:18:11: 5000000 INFO @ Sat, 24 Aug 2019 20:18:13: 2000000 INFO @ Sat, 24 Aug 2019 20:18:19: 9000000 INFO @ Sat, 24 Aug 2019 20:18:19: 6000000 INFO @ Sat, 24 Aug 2019 20:18:22: 3000000 INFO @ Sat, 24 Aug 2019 20:18:28: 7000000 INFO @ Sat, 24 Aug 2019 20:18:29: 10000000 INFO @ Sat, 24 Aug 2019 20:18:31: 4000000 INFO @ Sat, 24 Aug 2019 20:18:37: 8000000 INFO @ Sat, 24 Aug 2019 20:18:38: 11000000 INFO @ Sat, 24 Aug 2019 20:18:40: 5000000 INFO @ Sat, 24 Aug 2019 20:18:41: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:18:41: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:18:41: #1 total tags in treatment: 11249795 INFO @ Sat, 24 Aug 2019 20:18:41: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:18:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:18:41: #1 tags after filtering in treatment: 11249795 INFO @ Sat, 24 Aug 2019 20:18:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:18:41: #1 finished! INFO @ Sat, 24 Aug 2019 20:18:41: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:18:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:18:42: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:18:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:18:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:18:46: 9000000 INFO @ Sat, 24 Aug 2019 20:18:49: 6000000 INFO @ Sat, 24 Aug 2019 20:18:56: 10000000 INFO @ Sat, 24 Aug 2019 20:18:57: 7000000 INFO @ Sat, 24 Aug 2019 20:19:06: 11000000 INFO @ Sat, 24 Aug 2019 20:19:06: 8000000 INFO @ Sat, 24 Aug 2019 20:19:08: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:19:08: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:19:08: #1 total tags in treatment: 11249795 INFO @ Sat, 24 Aug 2019 20:19:08: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:19:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:19:09: #1 tags after filtering in treatment: 11249795 INFO @ Sat, 24 Aug 2019 20:19:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:19:09: #1 finished! INFO @ Sat, 24 Aug 2019 20:19:09: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:19:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:19:09: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:19:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:19:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:19:16: 9000000 INFO @ Sat, 24 Aug 2019 20:19:24: 10000000 INFO @ Sat, 24 Aug 2019 20:19:33: 11000000 INFO @ Sat, 24 Aug 2019 20:19:35: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:19:35: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:19:35: #1 total tags in treatment: 11249795 INFO @ Sat, 24 Aug 2019 20:19:35: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:19:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:19:35: #1 tags after filtering in treatment: 11249795 INFO @ Sat, 24 Aug 2019 20:19:35: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:19:35: #1 finished! INFO @ Sat, 24 Aug 2019 20:19:35: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:19:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:19:36: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:19:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:19:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292277/SRX4292277.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。