Job ID = 2640907 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,877,131 reads read : 5,877,131 reads written : 5,877,131 spots read : 6,494,385 reads read : 6,494,385 reads written : 6,494,385 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:30 12371516 reads; of these: 12371516 (100.00%) were unpaired; of these: 316050 (2.55%) aligned 0 times 9529509 (77.03%) aligned exactly 1 time 2525957 (20.42%) aligned >1 times 97.45% overall alignment rate Time searching: 00:02:30 Overall time: 00:02:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5504400 / 12055466 = 0.4566 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 20:00:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:00:17: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:00:17: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:00:25: 1000000 INFO @ Sat, 24 Aug 2019 20:00:32: 2000000 INFO @ Sat, 24 Aug 2019 20:00:40: 3000000 INFO @ Sat, 24 Aug 2019 20:00:47: 4000000 INFO @ Sat, 24 Aug 2019 20:00:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:00:47: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:00:47: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:00:54: 1000000 INFO @ Sat, 24 Aug 2019 20:00:54: 5000000 INFO @ Sat, 24 Aug 2019 20:01:01: 2000000 INFO @ Sat, 24 Aug 2019 20:01:01: 6000000 INFO @ Sat, 24 Aug 2019 20:01:05: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:01:05: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:01:05: #1 total tags in treatment: 6551066 INFO @ Sat, 24 Aug 2019 20:01:05: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:01:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:01:05: #1 tags after filtering in treatment: 6551066 INFO @ Sat, 24 Aug 2019 20:01:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:01:05: #1 finished! INFO @ Sat, 24 Aug 2019 20:01:05: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:01:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:01:06: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:01:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:01:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:01:07: 3000000 INFO @ Sat, 24 Aug 2019 20:01:14: 4000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 20:01:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 20:01:17: #1 read tag files... INFO @ Sat, 24 Aug 2019 20:01:17: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 20:01:20: 5000000 INFO @ Sat, 24 Aug 2019 20:01:26: 1000000 INFO @ Sat, 24 Aug 2019 20:01:27: 6000000 INFO @ Sat, 24 Aug 2019 20:01:31: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:01:31: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:01:31: #1 total tags in treatment: 6551066 INFO @ Sat, 24 Aug 2019 20:01:31: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:01:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:01:31: #1 tags after filtering in treatment: 6551066 INFO @ Sat, 24 Aug 2019 20:01:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:01:31: #1 finished! INFO @ Sat, 24 Aug 2019 20:01:31: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:01:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:01:31: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:01:31: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:01:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:01:36: 2000000 INFO @ Sat, 24 Aug 2019 20:01:44: 3000000 INFO @ Sat, 24 Aug 2019 20:01:53: 4000000 INFO @ Sat, 24 Aug 2019 20:02:01: 5000000 INFO @ Sat, 24 Aug 2019 20:02:10: 6000000 INFO @ Sat, 24 Aug 2019 20:02:14: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:02:14: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:02:14: #1 total tags in treatment: 6551066 INFO @ Sat, 24 Aug 2019 20:02:14: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:02:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:02:15: #1 tags after filtering in treatment: 6551066 INFO @ Sat, 24 Aug 2019 20:02:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:02:15: #1 finished! INFO @ Sat, 24 Aug 2019 20:02:15: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:02:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:02:15: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:02:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:02:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292274/SRX4292274.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。