Job ID = 2640906 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 4,401,424 reads read : 4,401,424 reads written : 4,401,424 spots read : 6,101,418 reads read : 6,101,418 reads written : 6,101,418 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:46 10502842 reads; of these: 10502842 (100.00%) were unpaired; of these: 204292 (1.95%) aligned 0 times 8025527 (76.41%) aligned exactly 1 time 2273023 (21.64%) aligned >1 times 98.05% overall alignment rate Time searching: 00:01:46 Overall time: 00:01:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4610806 / 10298550 = 0.4477 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:58:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:58:08: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:58:08: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:58:16: 1000000 INFO @ Sat, 24 Aug 2019 19:58:23: 2000000 INFO @ Sat, 24 Aug 2019 19:58:31: 3000000 INFO @ Sat, 24 Aug 2019 19:58:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:58:38: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:58:38: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:58:38: 4000000 INFO @ Sat, 24 Aug 2019 19:58:46: 1000000 INFO @ Sat, 24 Aug 2019 19:58:46: 5000000 INFO @ Sat, 24 Aug 2019 19:58:51: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:58:51: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:58:51: #1 total tags in treatment: 5687744 INFO @ Sat, 24 Aug 2019 19:58:51: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:58:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:58:51: #1 tags after filtering in treatment: 5687744 INFO @ Sat, 24 Aug 2019 19:58:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:58:51: #1 finished! INFO @ Sat, 24 Aug 2019 19:58:51: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:58:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:58:52: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:58:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:58:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:58:54: 2000000 INFO @ Sat, 24 Aug 2019 19:59:01: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:59:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:59:08: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:59:08: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:59:09: 4000000 INFO @ Sat, 24 Aug 2019 19:59:16: 5000000 INFO @ Sat, 24 Aug 2019 19:59:17: 1000000 INFO @ Sat, 24 Aug 2019 19:59:22: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:59:22: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:59:22: #1 total tags in treatment: 5687744 INFO @ Sat, 24 Aug 2019 19:59:22: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:59:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:59:22: #1 tags after filtering in treatment: 5687744 INFO @ Sat, 24 Aug 2019 19:59:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:59:22: #1 finished! INFO @ Sat, 24 Aug 2019 19:59:22: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:59:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:59:22: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:59:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:59:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:59:27: 2000000 INFO @ Sat, 24 Aug 2019 19:59:34: 3000000 INFO @ Sat, 24 Aug 2019 19:59:42: 4000000 INFO @ Sat, 24 Aug 2019 19:59:50: 5000000 INFO @ Sat, 24 Aug 2019 19:59:55: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:59:55: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:59:55: #1 total tags in treatment: 5687744 INFO @ Sat, 24 Aug 2019 19:59:55: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:59:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:59:55: #1 tags after filtering in treatment: 5687744 INFO @ Sat, 24 Aug 2019 19:59:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:59:55: #1 finished! INFO @ Sat, 24 Aug 2019 19:59:55: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:59:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:59:56: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:59:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:59:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292273/SRX4292273.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。