Job ID = 2640905 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,339,210 reads read : 6,339,210 reads written : 6,339,210 spots read : 4,560,266 reads read : 4,560,266 reads written : 4,560,266 fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:05 10899476 reads; of these: 10899476 (100.00%) were unpaired; of these: 174078 (1.60%) aligned 0 times 7989168 (73.30%) aligned exactly 1 time 2736230 (25.10%) aligned >1 times 98.40% overall alignment rate Time searching: 00:02:05 Overall time: 00:02:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5219640 / 10725398 = 0.4867 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:58:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:58:42: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:58:42: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:58:50: 1000000 INFO @ Sat, 24 Aug 2019 19:58:57: 2000000 INFO @ Sat, 24 Aug 2019 19:59:04: 3000000 INFO @ Sat, 24 Aug 2019 19:59:11: 4000000 INFO @ Sat, 24 Aug 2019 19:59:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:59:12: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:59:12: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:59:18: 5000000 INFO @ Sat, 24 Aug 2019 19:59:22: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:59:22: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:59:22: #1 total tags in treatment: 5505758 INFO @ Sat, 24 Aug 2019 19:59:22: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:59:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:59:22: 1000000 INFO @ Sat, 24 Aug 2019 19:59:22: #1 tags after filtering in treatment: 5505758 INFO @ Sat, 24 Aug 2019 19:59:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:59:22: #1 finished! INFO @ Sat, 24 Aug 2019 19:59:22: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:59:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:59:22: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:59:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:59:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:59:30: 2000000 INFO @ Sat, 24 Aug 2019 19:59:39: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:59:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:59:42: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:59:42: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:59:48: 4000000 INFO @ Sat, 24 Aug 2019 19:59:50: 1000000 INFO @ Sat, 24 Aug 2019 19:59:56: 5000000 INFO @ Sat, 24 Aug 2019 19:59:57: 2000000 INFO @ Sat, 24 Aug 2019 20:00:00: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:00:00: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:00:00: #1 total tags in treatment: 5505758 INFO @ Sat, 24 Aug 2019 20:00:00: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:00:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:00:00: #1 tags after filtering in treatment: 5505758 INFO @ Sat, 24 Aug 2019 20:00:00: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:00:00: #1 finished! INFO @ Sat, 24 Aug 2019 20:00:00: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:00:00: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:00:00: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:00:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:00:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 20:00:05: 3000000 INFO @ Sat, 24 Aug 2019 20:00:11: 4000000 INFO @ Sat, 24 Aug 2019 20:00:18: 5000000 INFO @ Sat, 24 Aug 2019 20:00:22: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 20:00:22: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 20:00:22: #1 total tags in treatment: 5505758 INFO @ Sat, 24 Aug 2019 20:00:22: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 20:00:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 20:00:22: #1 tags after filtering in treatment: 5505758 INFO @ Sat, 24 Aug 2019 20:00:22: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 20:00:22: #1 finished! INFO @ Sat, 24 Aug 2019 20:00:22: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 20:00:22: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 20:00:22: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 20:00:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 20:00:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292272/SRX4292272.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。