Job ID = 2640899 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,442,910 reads read : 6,442,910 reads written : 6,442,910 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:11 6442910 reads; of these: 6442910 (100.00%) were unpaired; of these: 641752 (9.96%) aligned 0 times 4225638 (65.59%) aligned exactly 1 time 1575520 (24.45%) aligned >1 times 90.04% overall alignment rate Time searching: 00:01:11 Overall time: 00:01:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1794586 / 5801158 = 0.3093 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:52:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:52:36: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:52:36: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:52:46: 1000000 INFO @ Sat, 24 Aug 2019 19:52:57: 2000000 INFO @ Sat, 24 Aug 2019 19:53:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:53:05: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:53:05: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:53:07: 3000000 INFO @ Sat, 24 Aug 2019 19:53:15: 1000000 INFO @ Sat, 24 Aug 2019 19:53:17: 4000000 INFO @ Sat, 24 Aug 2019 19:53:17: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:53:17: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:53:17: #1 total tags in treatment: 4006572 INFO @ Sat, 24 Aug 2019 19:53:17: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:53:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:53:17: #1 tags after filtering in treatment: 4006572 INFO @ Sat, 24 Aug 2019 19:53:17: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:53:17: #1 finished! INFO @ Sat, 24 Aug 2019 19:53:17: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:53:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:53:18: #2 number of paired peaks: 28 WARNING @ Sat, 24 Aug 2019 19:53:18: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:53:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:53:25: 2000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:53:34: 3000000 INFO @ Sat, 24 Aug 2019 19:53:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:53:35: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:53:35: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:53:44: 4000000 INFO @ Sat, 24 Aug 2019 19:53:44: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:53:44: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:53:44: #1 total tags in treatment: 4006572 INFO @ Sat, 24 Aug 2019 19:53:44: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:53:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:53:44: #1 tags after filtering in treatment: 4006572 INFO @ Sat, 24 Aug 2019 19:53:44: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:53:44: #1 finished! INFO @ Sat, 24 Aug 2019 19:53:44: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:53:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:53:44: #2 number of paired peaks: 28 WARNING @ Sat, 24 Aug 2019 19:53:44: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:53:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:53:45: 1000000 INFO @ Sat, 24 Aug 2019 19:53:54: 2000000 INFO @ Sat, 24 Aug 2019 19:54:03: 3000000 INFO @ Sat, 24 Aug 2019 19:54:12: 4000000 INFO @ Sat, 24 Aug 2019 19:54:12: #1 tag size is determined as 51 bps INFO @ Sat, 24 Aug 2019 19:54:12: #1 tag size = 51 INFO @ Sat, 24 Aug 2019 19:54:12: #1 total tags in treatment: 4006572 INFO @ Sat, 24 Aug 2019 19:54:12: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:54:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:54:12: #1 tags after filtering in treatment: 4006572 INFO @ Sat, 24 Aug 2019 19:54:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:54:12: #1 finished! INFO @ Sat, 24 Aug 2019 19:54:12: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:54:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:54:12: #2 number of paired peaks: 28 WARNING @ Sat, 24 Aug 2019 19:54:12: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:54:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4292266/SRX4292266.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。