Job ID = 11193118 sra ファイルのダウンロード中... Completed: 236982K bytes transferred in 6 seconds (282298K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 11807604 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4234139/SRR7360962.sra Written 11807604 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4234139/SRR7360962.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:14 11807604 reads; of these: 11807604 (100.00%) were unpaired; of these: 1330672 (11.27%) aligned 0 times 9573048 (81.08%) aligned exactly 1 time 903884 (7.66%) aligned >1 times 88.73% overall alignment rate Time searching: 00:02:14 Overall time: 00:02:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3102427 / 10476932 = 0.2961 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 11:02:57: # Command line: callpeak -t SRX4234139.bam -f BAM -g 12100000 -n SRX4234139.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4234139.05 # format = BAM # ChIP-seq file = ['SRX4234139.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:02:57: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:02:57: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:02:57: # Command line: callpeak -t SRX4234139.bam -f BAM -g 12100000 -n SRX4234139.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4234139.10 # format = BAM # ChIP-seq file = ['SRX4234139.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:02:57: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:02:57: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:02:57: # Command line: callpeak -t SRX4234139.bam -f BAM -g 12100000 -n SRX4234139.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4234139.20 # format = BAM # ChIP-seq file = ['SRX4234139.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:02:57: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:02:57: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:03:04: 1000000 INFO @ Sat, 15 Sep 2018 11:03:04: 1000000 INFO @ Sat, 15 Sep 2018 11:03:04: 1000000 INFO @ Sat, 15 Sep 2018 11:03:11: 2000000 INFO @ Sat, 15 Sep 2018 11:03:12: 2000000 INFO @ Sat, 15 Sep 2018 11:03:12: 2000000 INFO @ Sat, 15 Sep 2018 11:03:18: 3000000 INFO @ Sat, 15 Sep 2018 11:03:19: 3000000 INFO @ Sat, 15 Sep 2018 11:03:19: 3000000 INFO @ Sat, 15 Sep 2018 11:03:25: 4000000 INFO @ Sat, 15 Sep 2018 11:03:26: 4000000 INFO @ Sat, 15 Sep 2018 11:03:26: 4000000 INFO @ Sat, 15 Sep 2018 11:03:32: 5000000 INFO @ Sat, 15 Sep 2018 11:03:34: 5000000 INFO @ Sat, 15 Sep 2018 11:03:34: 5000000 INFO @ Sat, 15 Sep 2018 11:03:39: 6000000 INFO @ Sat, 15 Sep 2018 11:03:41: 6000000 INFO @ Sat, 15 Sep 2018 11:03:41: 6000000 INFO @ Sat, 15 Sep 2018 11:03:47: 7000000 INFO @ Sat, 15 Sep 2018 11:03:48: 7000000 INFO @ Sat, 15 Sep 2018 11:03:48: 7000000 INFO @ Sat, 15 Sep 2018 11:03:49: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:03:49: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:03:49: #1 total tags in treatment: 7374505 INFO @ Sat, 15 Sep 2018 11:03:49: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:03:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:03:49: #1 tags after filtering in treatment: 7374505 INFO @ Sat, 15 Sep 2018 11:03:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:03:49: #1 finished! INFO @ Sat, 15 Sep 2018 11:03:49: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:03:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:03:50: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:03:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:03:50: Process for pairing-model is terminated! cat: SRX4234139.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4234139.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4234139.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4234139.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:03:51: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:03:51: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:03:51: #1 total tags in treatment: 7374505 INFO @ Sat, 15 Sep 2018 11:03:51: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:03:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:03:51: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:03:51: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:03:51: #1 total tags in treatment: 7374505 INFO @ Sat, 15 Sep 2018 11:03:51: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:03:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:03:51: #1 tags after filtering in treatment: 7374505 INFO @ Sat, 15 Sep 2018 11:03:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:03:51: #1 finished! INFO @ Sat, 15 Sep 2018 11:03:51: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:03:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:03:51: #1 tags after filtering in treatment: 7374505 INFO @ Sat, 15 Sep 2018 11:03:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:03:51: #1 finished! INFO @ Sat, 15 Sep 2018 11:03:51: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:03:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:03:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:03:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:03:51: Process for pairing-model is terminated! cat: SRX4234139.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4234139.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4234139.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4234139.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:03:51: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:03:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:03:51: Process for pairing-model is terminated! cat: SRX4234139.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4234139.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4234139.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4234139.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。