Job ID = 11193085 sra ファイルのダウンロード中... Completed: 254930K bytes transferred in 6 seconds (338878K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 11492393 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233976/SRR7361125.sra Written 11492393 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233976/SRR7361125.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:53 11492393 reads; of these: 11492393 (100.00%) were unpaired; of these: 639442 (5.56%) aligned 0 times 10010595 (87.11%) aligned exactly 1 time 842356 (7.33%) aligned >1 times 94.44% overall alignment rate Time searching: 00:01:53 Overall time: 00:01:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2818685 / 10852951 = 0.2597 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:59:23: # Command line: callpeak -t SRX4233976.bam -f BAM -g 12100000 -n SRX4233976.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233976.10 # format = BAM # ChIP-seq file = ['SRX4233976.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:59:23: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:59:23: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:59:23: # Command line: callpeak -t SRX4233976.bam -f BAM -g 12100000 -n SRX4233976.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233976.05 # format = BAM # ChIP-seq file = ['SRX4233976.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:59:23: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:59:23: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:59:23: # Command line: callpeak -t SRX4233976.bam -f BAM -g 12100000 -n SRX4233976.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233976.20 # format = BAM # ChIP-seq file = ['SRX4233976.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:59:23: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:59:23: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:59:29: 1000000 INFO @ Sat, 15 Sep 2018 10:59:29: 1000000 INFO @ Sat, 15 Sep 2018 10:59:29: 1000000 INFO @ Sat, 15 Sep 2018 10:59:35: 2000000 INFO @ Sat, 15 Sep 2018 10:59:35: 2000000 INFO @ Sat, 15 Sep 2018 10:59:36: 2000000 INFO @ Sat, 15 Sep 2018 10:59:41: 3000000 INFO @ Sat, 15 Sep 2018 10:59:42: 3000000 INFO @ Sat, 15 Sep 2018 10:59:42: 3000000 INFO @ Sat, 15 Sep 2018 10:59:47: 4000000 INFO @ Sat, 15 Sep 2018 10:59:48: 4000000 INFO @ Sat, 15 Sep 2018 10:59:49: 4000000 INFO @ Sat, 15 Sep 2018 10:59:53: 5000000 INFO @ Sat, 15 Sep 2018 10:59:54: 5000000 INFO @ Sat, 15 Sep 2018 10:59:55: 5000000 INFO @ Sat, 15 Sep 2018 10:59:58: 6000000 INFO @ Sat, 15 Sep 2018 11:00:00: 6000000 INFO @ Sat, 15 Sep 2018 11:00:01: 6000000 INFO @ Sat, 15 Sep 2018 11:00:04: 7000000 INFO @ Sat, 15 Sep 2018 11:00:07: 7000000 INFO @ Sat, 15 Sep 2018 11:00:08: 7000000 INFO @ Sat, 15 Sep 2018 11:00:10: 8000000 INFO @ Sat, 15 Sep 2018 11:00:10: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:00:10: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:00:10: #1 total tags in treatment: 8034266 INFO @ Sat, 15 Sep 2018 11:00:10: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:00:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:00:11: #1 tags after filtering in treatment: 8034266 INFO @ Sat, 15 Sep 2018 11:00:11: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:00:11: #1 finished! INFO @ Sat, 15 Sep 2018 11:00:11: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:00:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:00:11: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:00:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:00:11: Process for pairing-model is terminated! cat: SRX4233976.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233976.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233976.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233976.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:00:13: 8000000 INFO @ Sat, 15 Sep 2018 11:00:13: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:00:13: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:00:13: #1 total tags in treatment: 8034266 INFO @ Sat, 15 Sep 2018 11:00:13: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:00:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:00:13: #1 tags after filtering in treatment: 8034266 INFO @ Sat, 15 Sep 2018 11:00:13: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:00:13: #1 finished! INFO @ Sat, 15 Sep 2018 11:00:13: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:00:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:00:14: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:00:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:00:14: Process for pairing-model is terminated! cat: SRX4233976.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233976.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233976.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233976.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:00:14: 8000000 INFO @ Sat, 15 Sep 2018 11:00:14: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:00:14: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:00:14: #1 total tags in treatment: 8034266 INFO @ Sat, 15 Sep 2018 11:00:14: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:00:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:00:15: #1 tags after filtering in treatment: 8034266 INFO @ Sat, 15 Sep 2018 11:00:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:00:15: #1 finished! INFO @ Sat, 15 Sep 2018 11:00:15: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:00:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:00:15: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:00:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:00:15: Process for pairing-model is terminated! cat: SRX4233976.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233976.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233976.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233976.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。