Job ID = 11193060 sra ファイルのダウンロード中... Completed: 233554K bytes transferred in 19 seconds (97434K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 11604936 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233898/SRR7361203.sra Written 11604936 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233898/SRR7361203.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:21 11604936 reads; of these: 11604936 (100.00%) were unpaired; of these: 683890 (5.89%) aligned 0 times 9619134 (82.89%) aligned exactly 1 time 1301912 (11.22%) aligned >1 times 94.11% overall alignment rate Time searching: 00:02:21 Overall time: 00:02:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3239838 / 10921046 = 0.2967 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:57:32: # Command line: callpeak -t SRX4233898.bam -f BAM -g 12100000 -n SRX4233898.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233898.05 # format = BAM # ChIP-seq file = ['SRX4233898.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:57:32: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:57:32: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:57:32: # Command line: callpeak -t SRX4233898.bam -f BAM -g 12100000 -n SRX4233898.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233898.20 # format = BAM # ChIP-seq file = ['SRX4233898.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:57:32: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:57:32: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:57:32: # Command line: callpeak -t SRX4233898.bam -f BAM -g 12100000 -n SRX4233898.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233898.10 # format = BAM # ChIP-seq file = ['SRX4233898.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:57:32: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:57:32: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:57:38: 1000000 INFO @ Sat, 15 Sep 2018 10:57:39: 1000000 INFO @ Sat, 15 Sep 2018 10:57:39: 1000000 INFO @ Sat, 15 Sep 2018 10:57:45: 2000000 INFO @ Sat, 15 Sep 2018 10:57:46: 2000000 INFO @ Sat, 15 Sep 2018 10:57:46: 2000000 INFO @ Sat, 15 Sep 2018 10:57:53: 3000000 INFO @ Sat, 15 Sep 2018 10:57:53: 3000000 INFO @ Sat, 15 Sep 2018 10:57:53: 3000000 INFO @ Sat, 15 Sep 2018 10:58:00: 4000000 INFO @ Sat, 15 Sep 2018 10:58:00: 4000000 INFO @ Sat, 15 Sep 2018 10:58:00: 4000000 INFO @ Sat, 15 Sep 2018 10:58:07: 5000000 INFO @ Sat, 15 Sep 2018 10:58:08: 5000000 INFO @ Sat, 15 Sep 2018 10:58:08: 5000000 INFO @ Sat, 15 Sep 2018 10:58:15: 6000000 INFO @ Sat, 15 Sep 2018 10:58:15: 6000000 INFO @ Sat, 15 Sep 2018 10:58:15: 6000000 INFO @ Sat, 15 Sep 2018 10:58:22: 7000000 INFO @ Sat, 15 Sep 2018 10:58:23: 7000000 INFO @ Sat, 15 Sep 2018 10:58:23: 7000000 INFO @ Sat, 15 Sep 2018 10:58:27: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:58:27: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:58:27: #1 total tags in treatment: 7681208 INFO @ Sat, 15 Sep 2018 10:58:27: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:58:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:58:27: #1 tags after filtering in treatment: 7681208 INFO @ Sat, 15 Sep 2018 10:58:27: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:58:27: #1 finished! INFO @ Sat, 15 Sep 2018 10:58:27: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:58:27: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:58:28: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:58:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:58:28: Process for pairing-model is terminated! cat: SRX4233898.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 6 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233898.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233898.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233898.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:58:28: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:58:28: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:58:28: #1 total tags in treatment: 7681208 INFO @ Sat, 15 Sep 2018 10:58:28: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:58:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:58:28: #1 tags after filtering in treatment: 7681208 INFO @ Sat, 15 Sep 2018 10:58:28: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:58:28: #1 finished! INFO @ Sat, 15 Sep 2018 10:58:28: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:58:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:58:28: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:58:28: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:58:28: #1 total tags in treatment: 7681208 INFO @ Sat, 15 Sep 2018 10:58:28: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:58:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:58:29: #1 tags after filtering in treatment: 7681208 INFO @ Sat, 15 Sep 2018 10:58:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:58:29: #1 finished! INFO @ Sat, 15 Sep 2018 10:58:29: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:58:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:58:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:58:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:58:29: Process for pairing-model is terminated! cat: SRX4233898.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233898.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233898.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233898.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:58:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:58:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:58:29: Process for pairing-model is terminated! cat: SRX4233898.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233898.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233898.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233898.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。