Job ID = 11193056 sra ファイルのダウンロード中... Completed: 138620K bytes transferred in 5 seconds (207447K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 7281826 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233894/SRR7360655.sra Written 7281826 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233894/SRR7360655.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:09 7281826 reads; of these: 7281826 (100.00%) were unpaired; of these: 1045416 (14.36%) aligned 0 times 5470074 (75.12%) aligned exactly 1 time 766336 (10.52%) aligned >1 times 85.64% overall alignment rate Time searching: 00:01:09 Overall time: 00:01:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2804511 / 6236410 = 0.4497 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:53:12: # Command line: callpeak -t SRX4233894.bam -f BAM -g 12100000 -n SRX4233894.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233894.05 # format = BAM # ChIP-seq file = ['SRX4233894.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:53:12: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:53:12: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:53:12: # Command line: callpeak -t SRX4233894.bam -f BAM -g 12100000 -n SRX4233894.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233894.20 # format = BAM # ChIP-seq file = ['SRX4233894.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:53:12: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:53:12: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:53:12: # Command line: callpeak -t SRX4233894.bam -f BAM -g 12100000 -n SRX4233894.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233894.10 # format = BAM # ChIP-seq file = ['SRX4233894.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:53:12: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:53:12: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:53:18: 1000000 INFO @ Sat, 15 Sep 2018 10:53:18: 1000000 INFO @ Sat, 15 Sep 2018 10:53:18: 1000000 INFO @ Sat, 15 Sep 2018 10:53:24: 2000000 INFO @ Sat, 15 Sep 2018 10:53:24: 2000000 INFO @ Sat, 15 Sep 2018 10:53:24: 2000000 INFO @ Sat, 15 Sep 2018 10:53:30: 3000000 INFO @ Sat, 15 Sep 2018 10:53:30: 3000000 INFO @ Sat, 15 Sep 2018 10:53:30: 3000000 INFO @ Sat, 15 Sep 2018 10:53:32: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:53:32: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:53:32: #1 total tags in treatment: 3431899 INFO @ Sat, 15 Sep 2018 10:53:32: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:32: #1 tags after filtering in treatment: 3431899 INFO @ Sat, 15 Sep 2018 10:53:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:32: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:32: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:33: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:53:33: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:53:33: #1 total tags in treatment: 3431899 INFO @ Sat, 15 Sep 2018 10:53:33: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:33: #2 number of paired peaks: 109 WARNING @ Sat, 15 Sep 2018 10:53:33: Fewer paired peaks (109) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 109 pairs to build model! INFO @ Sat, 15 Sep 2018 10:53:33: start model_add_line... INFO @ Sat, 15 Sep 2018 10:53:33: #1 tags after filtering in treatment: 3431899 INFO @ Sat, 15 Sep 2018 10:53:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:33: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:33: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:33: start X-correlation... INFO @ Sat, 15 Sep 2018 10:53:33: end of X-cor INFO @ Sat, 15 Sep 2018 10:53:33: #2 finished! INFO @ Sat, 15 Sep 2018 10:53:33: #2 predicted fragment length is 241 bps INFO @ Sat, 15 Sep 2018 10:53:33: #2 alternative fragment length(s) may be 3,114,142,186,216,223,241,248,273,295,304,309,343,365 bps INFO @ Sat, 15 Sep 2018 10:53:33: #2.2 Generate R script for model : SRX4233894.05_model.r INFO @ Sat, 15 Sep 2018 10:53:33: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:53:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:53:33: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:53:33: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:53:33: #1 total tags in treatment: 3431899 INFO @ Sat, 15 Sep 2018 10:53:33: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:33: #1 tags after filtering in treatment: 3431899 INFO @ Sat, 15 Sep 2018 10:53:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:33: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:33: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:33: #2 number of paired peaks: 109 WARNING @ Sat, 15 Sep 2018 10:53:33: Fewer paired peaks (109) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 109 pairs to build model! INFO @ Sat, 15 Sep 2018 10:53:33: start model_add_line... INFO @ Sat, 15 Sep 2018 10:53:33: start X-correlation... INFO @ Sat, 15 Sep 2018 10:53:33: end of X-cor INFO @ Sat, 15 Sep 2018 10:53:33: #2 finished! INFO @ Sat, 15 Sep 2018 10:53:33: #2 predicted fragment length is 241 bps INFO @ Sat, 15 Sep 2018 10:53:33: #2 alternative fragment length(s) may be 3,114,142,186,216,223,241,248,273,295,304,309,343,365 bps INFO @ Sat, 15 Sep 2018 10:53:33: #2.2 Generate R script for model : SRX4233894.10_model.r INFO @ Sat, 15 Sep 2018 10:53:33: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:53:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:53:33: #2 number of paired peaks: 109 WARNING @ Sat, 15 Sep 2018 10:53:33: Fewer paired peaks (109) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 109 pairs to build model! INFO @ Sat, 15 Sep 2018 10:53:33: start model_add_line... INFO @ Sat, 15 Sep 2018 10:53:33: start X-correlation... INFO @ Sat, 15 Sep 2018 10:53:33: end of X-cor INFO @ Sat, 15 Sep 2018 10:53:33: #2 finished! INFO @ Sat, 15 Sep 2018 10:53:33: #2 predicted fragment length is 241 bps INFO @ Sat, 15 Sep 2018 10:53:33: #2 alternative fragment length(s) may be 3,114,142,186,216,223,241,248,273,295,304,309,343,365 bps INFO @ Sat, 15 Sep 2018 10:53:33: #2.2 Generate R script for model : SRX4233894.20_model.r INFO @ Sat, 15 Sep 2018 10:53:33: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:53:33: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:53:47: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:53:47: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:53:48: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:53:50: #4 Write output xls file... SRX4233894.05_peaks.xls INFO @ Sat, 15 Sep 2018 10:53:50: #4 Write peak in narrowPeak format file... SRX4233894.05_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:53:50: #4 Write summits bed file... SRX4233894.05_summits.bed INFO @ Sat, 15 Sep 2018 10:53:50: Done! pass1 - making usageList (16 chroms): 0 millis pass2 - checking and writing primary data (1527 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:53:51: #4 Write output xls file... SRX4233894.10_peaks.xls INFO @ Sat, 15 Sep 2018 10:53:51: #4 Write peak in narrowPeak format file... SRX4233894.10_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:53:51: #4 Write summits bed file... SRX4233894.10_summits.bed INFO @ Sat, 15 Sep 2018 10:53:51: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (937 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:53:51: #4 Write output xls file... SRX4233894.20_peaks.xls INFO @ Sat, 15 Sep 2018 10:53:51: #4 Write peak in narrowPeak format file... SRX4233894.20_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:53:51: #4 Write summits bed file... SRX4233894.20_summits.bed INFO @ Sat, 15 Sep 2018 10:53:51: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (432 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。