Job ID = 11193041 sra ファイルのダウンロード中... Completed: 186135K bytes transferred in 4 seconds (318647K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 9221129 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233818/SRR7360731.sra Written 9221129 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233818/SRR7360731.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:48 9221129 reads; of these: 9221129 (100.00%) were unpaired; of these: 268106 (2.91%) aligned 0 times 8456919 (91.71%) aligned exactly 1 time 496104 (5.38%) aligned >1 times 97.09% overall alignment rate Time searching: 00:01:48 Overall time: 00:01:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2261097 / 8953023 = 0.2526 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:53:04: # Command line: callpeak -t SRX4233818.bam -f BAM -g 12100000 -n SRX4233818.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233818.10 # format = BAM # ChIP-seq file = ['SRX4233818.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:53:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:53:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:53:04: # Command line: callpeak -t SRX4233818.bam -f BAM -g 12100000 -n SRX4233818.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233818.05 # format = BAM # ChIP-seq file = ['SRX4233818.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:53:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:53:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:53:04: # Command line: callpeak -t SRX4233818.bam -f BAM -g 12100000 -n SRX4233818.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233818.20 # format = BAM # ChIP-seq file = ['SRX4233818.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:53:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:53:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:53:12: 1000000 INFO @ Sat, 15 Sep 2018 10:53:13: 1000000 INFO @ Sat, 15 Sep 2018 10:53:13: 1000000 INFO @ Sat, 15 Sep 2018 10:53:20: 2000000 INFO @ Sat, 15 Sep 2018 10:53:21: 2000000 INFO @ Sat, 15 Sep 2018 10:53:22: 2000000 INFO @ Sat, 15 Sep 2018 10:53:28: 3000000 INFO @ Sat, 15 Sep 2018 10:53:30: 3000000 INFO @ Sat, 15 Sep 2018 10:53:30: 3000000 INFO @ Sat, 15 Sep 2018 10:53:35: 4000000 INFO @ Sat, 15 Sep 2018 10:53:38: 4000000 INFO @ Sat, 15 Sep 2018 10:53:38: 4000000 INFO @ Sat, 15 Sep 2018 10:53:43: 5000000 INFO @ Sat, 15 Sep 2018 10:53:47: 5000000 INFO @ Sat, 15 Sep 2018 10:53:47: 5000000 INFO @ Sat, 15 Sep 2018 10:53:50: 6000000 INFO @ Sat, 15 Sep 2018 10:53:55: 6000000 INFO @ Sat, 15 Sep 2018 10:53:55: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:53:55: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:53:55: #1 total tags in treatment: 6691926 INFO @ Sat, 15 Sep 2018 10:53:55: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:55: 6000000 INFO @ Sat, 15 Sep 2018 10:53:56: #1 tags after filtering in treatment: 6691926 INFO @ Sat, 15 Sep 2018 10:53:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:56: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:56: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:53:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:53:56: Process for pairing-model is terminated! cat: SRX4233818.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233818.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233818.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233818.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:54:01: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:54:01: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:54:01: #1 total tags in treatment: 6691926 INFO @ Sat, 15 Sep 2018 10:54:01: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:54:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:54:01: #1 tags after filtering in treatment: 6691926 INFO @ Sat, 15 Sep 2018 10:54:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:54:01: #1 finished! INFO @ Sat, 15 Sep 2018 10:54:01: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:54:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:54:01: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:54:01: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:54:01: #1 total tags in treatment: 6691926 INFO @ Sat, 15 Sep 2018 10:54:01: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:54:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:54:01: #1 tags after filtering in treatment: 6691926 INFO @ Sat, 15 Sep 2018 10:54:01: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:54:01: #1 finished! INFO @ Sat, 15 Sep 2018 10:54:01: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:54:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:54:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:54:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:54:01: Process for pairing-model is terminated! cat: SRX4233818.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233818.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233818.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233818.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:54:01: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:54:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:54:01: Process for pairing-model is terminated! cat: SRX4233818.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233818.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233818.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233818.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。