Job ID = 11193039 sra ファイルのダウンロード中... Completed: 129792K bytes transferred in 5 seconds (202602K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 6433213 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233816/SRR7360733.sra Written 6433213 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233816/SRR7360733.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:04 6433213 reads; of these: 6433213 (100.00%) were unpaired; of these: 176173 (2.74%) aligned 0 times 5781954 (89.88%) aligned exactly 1 time 475086 (7.38%) aligned >1 times 97.26% overall alignment rate Time searching: 00:01:04 Overall time: 00:01:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1049209 / 6257040 = 0.1677 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:51:20: # Command line: callpeak -t SRX4233816.bam -f BAM -g 12100000 -n SRX4233816.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233816.10 # format = BAM # ChIP-seq file = ['SRX4233816.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:51:20: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:51:20: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:51:20: # Command line: callpeak -t SRX4233816.bam -f BAM -g 12100000 -n SRX4233816.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233816.20 # format = BAM # ChIP-seq file = ['SRX4233816.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:51:20: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:51:20: # Command line: callpeak -t SRX4233816.bam -f BAM -g 12100000 -n SRX4233816.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233816.05 # format = BAM # ChIP-seq file = ['SRX4233816.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:51:20: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:51:20: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:51:20: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:51:27: 1000000 INFO @ Sat, 15 Sep 2018 10:51:27: 1000000 INFO @ Sat, 15 Sep 2018 10:51:27: 1000000 INFO @ Sat, 15 Sep 2018 10:51:33: 2000000 INFO @ Sat, 15 Sep 2018 10:51:34: 2000000 INFO @ Sat, 15 Sep 2018 10:51:34: 2000000 INFO @ Sat, 15 Sep 2018 10:51:40: 3000000 INFO @ Sat, 15 Sep 2018 10:51:40: 3000000 INFO @ Sat, 15 Sep 2018 10:51:41: 3000000 INFO @ Sat, 15 Sep 2018 10:51:46: 4000000 INFO @ Sat, 15 Sep 2018 10:51:47: 4000000 INFO @ Sat, 15 Sep 2018 10:51:47: 4000000 INFO @ Sat, 15 Sep 2018 10:51:53: 5000000 INFO @ Sat, 15 Sep 2018 10:51:54: 5000000 INFO @ Sat, 15 Sep 2018 10:51:54: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:51:54: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:51:54: #1 total tags in treatment: 5207831 INFO @ Sat, 15 Sep 2018 10:51:54: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:51:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:51:54: #1 tags after filtering in treatment: 5207831 INFO @ Sat, 15 Sep 2018 10:51:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:51:54: #1 finished! INFO @ Sat, 15 Sep 2018 10:51:54: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:51:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:51:54: 5000000 INFO @ Sat, 15 Sep 2018 10:51:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:51:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:51:54: Process for pairing-model is terminated! cat: SRX4233816.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233816.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233816.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233816.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:51:55: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:51:55: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:51:55: #1 total tags in treatment: 5207831 INFO @ Sat, 15 Sep 2018 10:51:55: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:51:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:51:55: #1 tags after filtering in treatment: 5207831 INFO @ Sat, 15 Sep 2018 10:51:55: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:51:55: #1 finished! INFO @ Sat, 15 Sep 2018 10:51:55: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:51:55: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:51:55: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:51:55: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:51:55: #1 total tags in treatment: 5207831 INFO @ Sat, 15 Sep 2018 10:51:55: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:51:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:51:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:51:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:51:55: Process for pairing-model is terminated! cat: SRX4233816.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233816.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233816.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233816.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:51:56: #1 tags after filtering in treatment: 5207831 INFO @ Sat, 15 Sep 2018 10:51:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:51:56: #1 finished! INFO @ Sat, 15 Sep 2018 10:51:56: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:51:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:51:56: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:51:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:51:56: Process for pairing-model is terminated! cat: SRX4233816.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233816.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233816.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233816.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。