Job ID = 11193034 sra ファイルのダウンロード中... Completed: 26207K bytes transferred in 3 seconds (63943K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 1247692 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233811/SRR7360738.sra Written 1247692 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233811/SRR7360738.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:14 1247692 reads; of these: 1247692 (100.00%) were unpaired; of these: 67162 (5.38%) aligned 0 times 1045617 (83.80%) aligned exactly 1 time 134913 (10.81%) aligned >1 times 94.62% overall alignment rate Time searching: 00:00:14 Overall time: 00:00:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 81464 / 1180530 = 0.0690 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:48:56: # Command line: callpeak -t SRX4233811.bam -f BAM -g 12100000 -n SRX4233811.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233811.20 # format = BAM # ChIP-seq file = ['SRX4233811.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:48:56: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:48:56: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:48:56: # Command line: callpeak -t SRX4233811.bam -f BAM -g 12100000 -n SRX4233811.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233811.05 # format = BAM # ChIP-seq file = ['SRX4233811.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:48:56: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:48:56: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:48:56: # Command line: callpeak -t SRX4233811.bam -f BAM -g 12100000 -n SRX4233811.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233811.10 # format = BAM # ChIP-seq file = ['SRX4233811.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:48:56: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:48:56: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:49:04: 1000000 INFO @ Sat, 15 Sep 2018 10:49:04: 1000000 INFO @ Sat, 15 Sep 2018 10:49:04: 1000000 INFO @ Sat, 15 Sep 2018 10:49:05: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:49:05: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:49:05: #1 total tags in treatment: 1099066 INFO @ Sat, 15 Sep 2018 10:49:05: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:49:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:49:05: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:49:05: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:49:05: #1 total tags in treatment: 1099066 INFO @ Sat, 15 Sep 2018 10:49:05: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:49:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:49:05: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:49:05: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:49:05: #1 total tags in treatment: 1099066 INFO @ Sat, 15 Sep 2018 10:49:05: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:49:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:49:05: #1 tags after filtering in treatment: 1099066 INFO @ Sat, 15 Sep 2018 10:49:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:49:05: #1 finished! INFO @ Sat, 15 Sep 2018 10:49:05: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:49:05: #1 tags after filtering in treatment: 1099066 INFO @ Sat, 15 Sep 2018 10:49:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:49:05: #1 finished! INFO @ Sat, 15 Sep 2018 10:49:05: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:49:05: #1 tags after filtering in treatment: 1099066 INFO @ Sat, 15 Sep 2018 10:49:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:49:05: #1 finished! INFO @ Sat, 15 Sep 2018 10:49:05: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:49:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:49:05: #2 number of paired peaks: 66 WARNING @ Sat, 15 Sep 2018 10:49:05: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:49:05: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 10:49:05: #2 number of paired peaks: 66 WARNING @ Sat, 15 Sep 2018 10:49:05: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:49:05: Process for pairing-model is terminated! cat: SRX4233811.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Sat, 15 Sep 2018 10:49:05: #2 number of paired peaks: 66 WARNING @ Sat, 15 Sep 2018 10:49:05: Too few paired peaks (66) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:49:05: Process for pairing-model is terminated! cat: SRX4233811.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis cat: SRX4233811.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX4233811.05_model.r': そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX4233811.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233811.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling rm: cannot remove `SRX4233811.20_model.r'rm: cannot remove `SRX4233811.10_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233811.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233811.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233811.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233811.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。