Job ID = 11193033 sra ファイルのダウンロード中... Completed: 189878K bytes transferred in 5 seconds (289515K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 9459633 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233810/SRR7360739.sra Written 9459633 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233810/SRR7360739.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:40 9459633 reads; of these: 9459633 (100.00%) were unpaired; of these: 1144837 (12.10%) aligned 0 times 7315693 (77.34%) aligned exactly 1 time 999103 (10.56%) aligned >1 times 87.90% overall alignment rate Time searching: 00:01:40 Overall time: 00:01:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2621719 / 8314796 = 0.3153 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:52:38: # Command line: callpeak -t SRX4233810.bam -f BAM -g 12100000 -n SRX4233810.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233810.05 # format = BAM # ChIP-seq file = ['SRX4233810.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:38: # Command line: callpeak -t SRX4233810.bam -f BAM -g 12100000 -n SRX4233810.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233810.10 # format = BAM # ChIP-seq file = ['SRX4233810.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:38: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:38: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:38: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:38: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:38: # Command line: callpeak -t SRX4233810.bam -f BAM -g 12100000 -n SRX4233810.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233810.20 # format = BAM # ChIP-seq file = ['SRX4233810.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:38: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:38: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:47: 1000000 INFO @ Sat, 15 Sep 2018 10:52:47: 1000000 INFO @ Sat, 15 Sep 2018 10:52:47: 1000000 INFO @ Sat, 15 Sep 2018 10:52:56: 2000000 INFO @ Sat, 15 Sep 2018 10:52:56: 2000000 INFO @ Sat, 15 Sep 2018 10:52:56: 2000000 INFO @ Sat, 15 Sep 2018 10:53:05: 3000000 INFO @ Sat, 15 Sep 2018 10:53:05: 3000000 INFO @ Sat, 15 Sep 2018 10:53:05: 3000000 INFO @ Sat, 15 Sep 2018 10:53:14: 4000000 INFO @ Sat, 15 Sep 2018 10:53:14: 4000000 INFO @ Sat, 15 Sep 2018 10:53:14: 4000000 INFO @ Sat, 15 Sep 2018 10:53:23: 5000000 INFO @ Sat, 15 Sep 2018 10:53:23: 5000000 INFO @ Sat, 15 Sep 2018 10:53:24: 5000000 INFO @ Sat, 15 Sep 2018 10:53:29: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:53:29: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:53:29: #1 total tags in treatment: 5693077 INFO @ Sat, 15 Sep 2018 10:53:29: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:29: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:53:29: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:53:29: #1 total tags in treatment: 5693077 INFO @ Sat, 15 Sep 2018 10:53:29: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:29: #1 tags after filtering in treatment: 5693077 INFO @ Sat, 15 Sep 2018 10:53:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:29: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:29: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:29: #1 tags after filtering in treatment: 5693077 INFO @ Sat, 15 Sep 2018 10:53:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:29: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:29: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:53:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:53:29: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 10:53:29: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:53:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:53:29: Process for pairing-model is terminated! cat: cat: SRX4233810.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません SRX4233810.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms)pass1 - making usageList (0 chroms): 20 millis : 20 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184)needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233810.10_model.r'rm: cannot remove `SRX4233810.05_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233810.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233810.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233810.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233810.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:53:31: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:53:31: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:53:31: #1 total tags in treatment: 5693077 INFO @ Sat, 15 Sep 2018 10:53:31: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:32: #1 tags after filtering in treatment: 5693077 INFO @ Sat, 15 Sep 2018 10:53:32: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:32: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:32: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:32: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:32: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:53:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:53:32: Process for pairing-model is terminated! cat: SRX4233810.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233810.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233810.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233810.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。