Job ID = 11193028 sra ファイルのダウンロード中... Completed: 266470K bytes transferred in 6 seconds (321049K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 13334375 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233804/SRR7360745.sra Written 13334375 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233804/SRR7360745.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:08 13334375 reads; of these: 13334375 (100.00%) were unpaired; of these: 936690 (7.02%) aligned 0 times 10854661 (81.40%) aligned exactly 1 time 1543024 (11.57%) aligned >1 times 92.98% overall alignment rate Time searching: 00:02:08 Overall time: 00:02:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4202408 / 12397685 = 0.3390 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:52:52: # Command line: callpeak -t SRX4233804.bam -f BAM -g 12100000 -n SRX4233804.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233804.05 # format = BAM # ChIP-seq file = ['SRX4233804.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:52: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:52: # Command line: callpeak -t SRX4233804.bam -f BAM -g 12100000 -n SRX4233804.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233804.10 # format = BAM # ChIP-seq file = ['SRX4233804.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:52: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:52: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:52: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:52: # Command line: callpeak -t SRX4233804.bam -f BAM -g 12100000 -n SRX4233804.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233804.20 # format = BAM # ChIP-seq file = ['SRX4233804.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:52: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:52: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:57: 1000000 INFO @ Sat, 15 Sep 2018 10:52:58: 1000000 INFO @ Sat, 15 Sep 2018 10:52:58: 1000000 INFO @ Sat, 15 Sep 2018 10:53:03: 2000000 INFO @ Sat, 15 Sep 2018 10:53:03: 2000000 INFO @ Sat, 15 Sep 2018 10:53:04: 2000000 INFO @ Sat, 15 Sep 2018 10:53:09: 3000000 INFO @ Sat, 15 Sep 2018 10:53:09: 3000000 INFO @ Sat, 15 Sep 2018 10:53:09: 3000000 INFO @ Sat, 15 Sep 2018 10:53:15: 4000000 INFO @ Sat, 15 Sep 2018 10:53:15: 4000000 INFO @ Sat, 15 Sep 2018 10:53:15: 4000000 INFO @ Sat, 15 Sep 2018 10:53:21: 5000000 INFO @ Sat, 15 Sep 2018 10:53:21: 5000000 INFO @ Sat, 15 Sep 2018 10:53:21: 5000000 INFO @ Sat, 15 Sep 2018 10:53:26: 6000000 INFO @ Sat, 15 Sep 2018 10:53:27: 6000000 INFO @ Sat, 15 Sep 2018 10:53:27: 6000000 INFO @ Sat, 15 Sep 2018 10:53:32: 7000000 INFO @ Sat, 15 Sep 2018 10:53:33: 7000000 INFO @ Sat, 15 Sep 2018 10:53:33: 7000000 INFO @ Sat, 15 Sep 2018 10:53:38: 8000000 INFO @ Sat, 15 Sep 2018 10:53:39: 8000000 INFO @ Sat, 15 Sep 2018 10:53:39: 8000000 INFO @ Sat, 15 Sep 2018 10:53:39: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:53:39: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:53:39: #1 total tags in treatment: 8195277 INFO @ Sat, 15 Sep 2018 10:53:39: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:39: #1 tags after filtering in treatment: 8195277 INFO @ Sat, 15 Sep 2018 10:53:39: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:39: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:39: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:40: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:53:40: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:53:40: #1 total tags in treatment: 8195277 INFO @ Sat, 15 Sep 2018 10:53:40: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:53:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:53:40: Process for pairing-model is terminated! cat: SRX4233804.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233804.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233804.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233804.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:53:40: #1 tags after filtering in treatment: 8195277 INFO @ Sat, 15 Sep 2018 10:53:40: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:40: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:40: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:40: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:53:40: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:53:40: #1 total tags in treatment: 8195277 INFO @ Sat, 15 Sep 2018 10:53:40: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:53:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:53:40: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:53:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:53:40: Process for pairing-model is terminated! cat: SRX4233804.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233804.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233804.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233804.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:53:41: #1 tags after filtering in treatment: 8195277 INFO @ Sat, 15 Sep 2018 10:53:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:53:41: #1 finished! INFO @ Sat, 15 Sep 2018 10:53:41: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:53:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:53:41: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:53:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:53:41: Process for pairing-model is terminated! cat: SRX4233804.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233804.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233804.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233804.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。