Job ID = 11193014 sra ファイルのダウンロード中... Completed: 462261K bytes transferred in 8 seconds (430226K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 23010266 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233789/SRR7360760.sra Written 23010266 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233789/SRR7360760.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:46 23010266 reads; of these: 23010266 (100.00%) were unpaired; of these: 1217341 (5.29%) aligned 0 times 20099282 (87.35%) aligned exactly 1 time 1693643 (7.36%) aligned >1 times 94.71% overall alignment rate Time searching: 00:03:46 Overall time: 00:03:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9144279 / 21792925 = 0.4196 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:55:33: # Command line: callpeak -t SRX4233789.bam -f BAM -g 12100000 -n SRX4233789.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233789.20 # format = BAM # ChIP-seq file = ['SRX4233789.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:55:33: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:55:33: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:55:33: # Command line: callpeak -t SRX4233789.bam -f BAM -g 12100000 -n SRX4233789.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233789.05 # format = BAM # ChIP-seq file = ['SRX4233789.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:55:33: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:55:33: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:55:33: # Command line: callpeak -t SRX4233789.bam -f BAM -g 12100000 -n SRX4233789.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233789.10 # format = BAM # ChIP-seq file = ['SRX4233789.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:55:33: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:55:33: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:55:39: 1000000 INFO @ Sat, 15 Sep 2018 10:55:39: 1000000 INFO @ Sat, 15 Sep 2018 10:55:39: 1000000 INFO @ Sat, 15 Sep 2018 10:55:45: 2000000 INFO @ Sat, 15 Sep 2018 10:55:45: 2000000 INFO @ Sat, 15 Sep 2018 10:55:45: 2000000 INFO @ Sat, 15 Sep 2018 10:55:52: 3000000 INFO @ Sat, 15 Sep 2018 10:55:52: 3000000 INFO @ Sat, 15 Sep 2018 10:55:52: 3000000 INFO @ Sat, 15 Sep 2018 10:55:58: 4000000 INFO @ Sat, 15 Sep 2018 10:55:58: 4000000 INFO @ Sat, 15 Sep 2018 10:55:58: 4000000 INFO @ Sat, 15 Sep 2018 10:56:04: 5000000 INFO @ Sat, 15 Sep 2018 10:56:05: 5000000 INFO @ Sat, 15 Sep 2018 10:56:05: 5000000 INFO @ Sat, 15 Sep 2018 10:56:11: 6000000 INFO @ Sat, 15 Sep 2018 10:56:11: 6000000 INFO @ Sat, 15 Sep 2018 10:56:11: 6000000 INFO @ Sat, 15 Sep 2018 10:56:17: 7000000 INFO @ Sat, 15 Sep 2018 10:56:17: 7000000 INFO @ Sat, 15 Sep 2018 10:56:17: 7000000 INFO @ Sat, 15 Sep 2018 10:56:24: 8000000 INFO @ Sat, 15 Sep 2018 10:56:24: 8000000 INFO @ Sat, 15 Sep 2018 10:56:24: 8000000 INFO @ Sat, 15 Sep 2018 10:56:30: 9000000 INFO @ Sat, 15 Sep 2018 10:56:30: 9000000 INFO @ Sat, 15 Sep 2018 10:56:30: 9000000 INFO @ Sat, 15 Sep 2018 10:56:36: 10000000 INFO @ Sat, 15 Sep 2018 10:56:37: 10000000 INFO @ Sat, 15 Sep 2018 10:56:37: 10000000 INFO @ Sat, 15 Sep 2018 10:56:43: 11000000 INFO @ Sat, 15 Sep 2018 10:56:43: 11000000 INFO @ Sat, 15 Sep 2018 10:56:43: 11000000 INFO @ Sat, 15 Sep 2018 10:56:49: 12000000 INFO @ Sat, 15 Sep 2018 10:56:49: 12000000 INFO @ Sat, 15 Sep 2018 10:56:50: 12000000 INFO @ Sat, 15 Sep 2018 10:56:53: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:56:53: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:56:53: #1 total tags in treatment: 12648646 INFO @ Sat, 15 Sep 2018 10:56:53: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:56:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:56:53: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:56:53: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:56:53: #1 total tags in treatment: 12648646 INFO @ Sat, 15 Sep 2018 10:56:53: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:56:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:56:54: #1 tags after filtering in treatment: 12648646 INFO @ Sat, 15 Sep 2018 10:56:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:56:54: #1 finished! INFO @ Sat, 15 Sep 2018 10:56:54: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:56:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:56:54: #1 tags after filtering in treatment: 12648646 INFO @ Sat, 15 Sep 2018 10:56:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:56:54: #1 finished! INFO @ Sat, 15 Sep 2018 10:56:54: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:56:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:56:54: #1 tag size is determined as 48 bps INFO @ Sat, 15 Sep 2018 10:56:54: #1 tag size = 48 INFO @ Sat, 15 Sep 2018 10:56:54: #1 total tags in treatment: 12648646 INFO @ Sat, 15 Sep 2018 10:56:54: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:56:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:56:54: #1 tags after filtering in treatment: 12648646 INFO @ Sat, 15 Sep 2018 10:56:54: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:56:54: #1 finished! INFO @ Sat, 15 Sep 2018 10:56:54: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:56:54: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:56:54: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:56:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:56:54: Process for pairing-model is terminated! cat: SRX4233789.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233789.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233789.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233789.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:56:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:56:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:56:55: Process for pairing-model is terminated! cat: SRX4233789.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233789.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233789.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233789.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:56:55: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:56:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:56:55: Process for pairing-model is terminated! cat: SRX4233789.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233789.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233789.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233789.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。