Job ID = 11193002 sra ファイルのダウンロード中... Completed: 349065K bytes transferred in 26 seconds (106193K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 17328422 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233730/SRR7360819.sra Written 17328422 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233730/SRR7360819.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:20 17328422 reads; of these: 17328422 (100.00%) were unpaired; of these: 889784 (5.13%) aligned 0 times 15561530 (89.80%) aligned exactly 1 time 877108 (5.06%) aligned >1 times 94.87% overall alignment rate Time searching: 00:03:20 Overall time: 00:03:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6739751 / 16438638 = 0.4100 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:52:56: # Command line: callpeak -t SRX4233730.bam -f BAM -g 12100000 -n SRX4233730.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233730.05 # format = BAM # ChIP-seq file = ['SRX4233730.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:56: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:56: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:56: # Command line: callpeak -t SRX4233730.bam -f BAM -g 12100000 -n SRX4233730.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233730.20 # format = BAM # ChIP-seq file = ['SRX4233730.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:56: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:56: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:52:56: # Command line: callpeak -t SRX4233730.bam -f BAM -g 12100000 -n SRX4233730.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233730.10 # format = BAM # ChIP-seq file = ['SRX4233730.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:52:56: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:52:56: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:53:05: 1000000 INFO @ Sat, 15 Sep 2018 10:53:05: 1000000 INFO @ Sat, 15 Sep 2018 10:53:05: 1000000 INFO @ Sat, 15 Sep 2018 10:53:13: 2000000 INFO @ Sat, 15 Sep 2018 10:53:14: 2000000 INFO @ Sat, 15 Sep 2018 10:53:14: 2000000 INFO @ Sat, 15 Sep 2018 10:53:22: 3000000 INFO @ Sat, 15 Sep 2018 10:53:23: 3000000 INFO @ Sat, 15 Sep 2018 10:53:23: 3000000 INFO @ Sat, 15 Sep 2018 10:53:31: 4000000 INFO @ Sat, 15 Sep 2018 10:53:32: 4000000 INFO @ Sat, 15 Sep 2018 10:53:32: 4000000 INFO @ Sat, 15 Sep 2018 10:53:39: 5000000 INFO @ Sat, 15 Sep 2018 10:53:40: 5000000 INFO @ Sat, 15 Sep 2018 10:53:40: 5000000 INFO @ Sat, 15 Sep 2018 10:53:46: 6000000 INFO @ Sat, 15 Sep 2018 10:53:49: 6000000 INFO @ Sat, 15 Sep 2018 10:53:49: 6000000 INFO @ Sat, 15 Sep 2018 10:53:54: 7000000 INFO @ Sat, 15 Sep 2018 10:53:58: 7000000 INFO @ Sat, 15 Sep 2018 10:53:58: 7000000 INFO @ Sat, 15 Sep 2018 10:54:02: 8000000 INFO @ Sat, 15 Sep 2018 10:54:07: 8000000 INFO @ Sat, 15 Sep 2018 10:54:07: 8000000 INFO @ Sat, 15 Sep 2018 10:54:09: 9000000 INFO @ Sat, 15 Sep 2018 10:54:15: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:54:15: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:54:15: #1 total tags in treatment: 9698887 INFO @ Sat, 15 Sep 2018 10:54:15: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:54:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:54:15: 9000000 INFO @ Sat, 15 Sep 2018 10:54:15: 9000000 INFO @ Sat, 15 Sep 2018 10:54:15: #1 tags after filtering in treatment: 9698887 INFO @ Sat, 15 Sep 2018 10:54:15: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:54:15: #1 finished! INFO @ Sat, 15 Sep 2018 10:54:15: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:54:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:54:16: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:54:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:54:16: Process for pairing-model is terminated! cat: SRX4233730.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233730.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233730.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233730.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:54:21: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:54:21: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:54:21: #1 total tags in treatment: 9698887 INFO @ Sat, 15 Sep 2018 10:54:21: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:54:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:54:21: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 10:54:21: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 10:54:21: #1 total tags in treatment: 9698887 INFO @ Sat, 15 Sep 2018 10:54:21: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:54:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:54:21: #1 tags after filtering in treatment: 9698887 INFO @ Sat, 15 Sep 2018 10:54:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:54:21: #1 finished! INFO @ Sat, 15 Sep 2018 10:54:21: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:54:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:54:21: #1 tags after filtering in treatment: 9698887 INFO @ Sat, 15 Sep 2018 10:54:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:54:21: #1 finished! INFO @ Sat, 15 Sep 2018 10:54:21: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:54:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:54:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:54:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:54:22: Process for pairing-model is terminated! cat: SRX4233730.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233730.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233730.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233730.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:54:22: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 10:54:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:54:22: Process for pairing-model is terminated! cat: SRX4233730.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233730.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233730.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233730.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。