Job ID = 11192995 sra ファイルのダウンロード中... Completed: 928175K bytes transferred in 15 seconds (495455K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 41548400 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233703/SRR7360846.sra Written 41548400 spots for /home/okishinya/chipatlas/results/sacCer3/SRX4233703/SRR7360846.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:27 41548400 reads; of these: 41548400 (100.00%) were unpaired; of these: 4689810 (11.29%) aligned 0 times 34638646 (83.37%) aligned exactly 1 time 2219944 (5.34%) aligned >1 times 88.71% overall alignment rate Time searching: 00:08:27 Overall time: 00:08:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 21561634 / 36858590 = 0.5850 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 11:03:55: # Command line: callpeak -t SRX4233703.bam -f BAM -g 12100000 -n SRX4233703.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX4233703.05 # format = BAM # ChIP-seq file = ['SRX4233703.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:03:55: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:03:55: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:03:55: # Command line: callpeak -t SRX4233703.bam -f BAM -g 12100000 -n SRX4233703.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX4233703.10 # format = BAM # ChIP-seq file = ['SRX4233703.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:03:55: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:03:55: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:03:55: # Command line: callpeak -t SRX4233703.bam -f BAM -g 12100000 -n SRX4233703.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX4233703.20 # format = BAM # ChIP-seq file = ['SRX4233703.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 11:03:55: #1 read tag files... INFO @ Sat, 15 Sep 2018 11:03:55: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 11:04:03: 1000000 INFO @ Sat, 15 Sep 2018 11:04:03: 1000000 INFO @ Sat, 15 Sep 2018 11:04:03: 1000000 INFO @ Sat, 15 Sep 2018 11:04:11: 2000000 INFO @ Sat, 15 Sep 2018 11:04:11: 2000000 INFO @ Sat, 15 Sep 2018 11:04:11: 2000000 INFO @ Sat, 15 Sep 2018 11:04:18: 3000000 INFO @ Sat, 15 Sep 2018 11:04:20: 3000000 INFO @ Sat, 15 Sep 2018 11:04:20: 3000000 INFO @ Sat, 15 Sep 2018 11:04:25: 4000000 INFO @ Sat, 15 Sep 2018 11:04:28: 4000000 INFO @ Sat, 15 Sep 2018 11:04:28: 4000000 INFO @ Sat, 15 Sep 2018 11:04:32: 5000000 INFO @ Sat, 15 Sep 2018 11:04:36: 5000000 INFO @ Sat, 15 Sep 2018 11:04:36: 5000000 INFO @ Sat, 15 Sep 2018 11:04:39: 6000000 INFO @ Sat, 15 Sep 2018 11:04:44: 6000000 INFO @ Sat, 15 Sep 2018 11:04:44: 6000000 INFO @ Sat, 15 Sep 2018 11:04:46: 7000000 INFO @ Sat, 15 Sep 2018 11:04:52: 7000000 INFO @ Sat, 15 Sep 2018 11:04:52: 7000000 INFO @ Sat, 15 Sep 2018 11:04:53: 8000000 INFO @ Sat, 15 Sep 2018 11:05:00: 8000000 INFO @ Sat, 15 Sep 2018 11:05:00: 8000000 INFO @ Sat, 15 Sep 2018 11:05:00: 9000000 INFO @ Sat, 15 Sep 2018 11:05:07: 9000000 INFO @ Sat, 15 Sep 2018 11:05:07: 9000000 INFO @ Sat, 15 Sep 2018 11:05:08: 10000000 INFO @ Sat, 15 Sep 2018 11:05:15: 11000000 INFO @ Sat, 15 Sep 2018 11:05:15: 10000000 INFO @ Sat, 15 Sep 2018 11:05:15: 10000000 INFO @ Sat, 15 Sep 2018 11:05:22: 12000000 INFO @ Sat, 15 Sep 2018 11:05:23: 11000000 INFO @ Sat, 15 Sep 2018 11:05:23: 11000000 INFO @ Sat, 15 Sep 2018 11:05:30: 13000000 INFO @ Sat, 15 Sep 2018 11:05:32: 12000000 INFO @ Sat, 15 Sep 2018 11:05:32: 12000000 INFO @ Sat, 15 Sep 2018 11:05:37: 14000000 INFO @ Sat, 15 Sep 2018 11:05:40: 13000000 INFO @ Sat, 15 Sep 2018 11:05:40: 13000000 INFO @ Sat, 15 Sep 2018 11:05:44: 15000000 INFO @ Sat, 15 Sep 2018 11:05:46: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:05:46: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:05:46: #1 total tags in treatment: 15296956 INFO @ Sat, 15 Sep 2018 11:05:46: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:05:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:05:46: #1 tags after filtering in treatment: 15296956 INFO @ Sat, 15 Sep 2018 11:05:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:05:46: #1 finished! INFO @ Sat, 15 Sep 2018 11:05:46: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:05:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:05:47: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:05:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:05:47: Process for pairing-model is terminated! cat: SRX4233703.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233703.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233703.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233703.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 11:05:48: 14000000 INFO @ Sat, 15 Sep 2018 11:05:48: 14000000 INFO @ Sat, 15 Sep 2018 11:05:55: 15000000 INFO @ Sat, 15 Sep 2018 11:05:55: 15000000 INFO @ Sat, 15 Sep 2018 11:05:57: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:05:57: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:05:57: #1 total tags in treatment: 15296956 INFO @ Sat, 15 Sep 2018 11:05:57: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:05:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:05:57: #1 tag size is determined as 49 bps INFO @ Sat, 15 Sep 2018 11:05:57: #1 tag size = 49 INFO @ Sat, 15 Sep 2018 11:05:57: #1 total tags in treatment: 15296956 INFO @ Sat, 15 Sep 2018 11:05:57: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 11:05:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 11:05:57: #1 tags after filtering in treatment: 15296956 INFO @ Sat, 15 Sep 2018 11:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:05:57: #1 finished! INFO @ Sat, 15 Sep 2018 11:05:57: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:05:57: #1 tags after filtering in treatment: 15296956 INFO @ Sat, 15 Sep 2018 11:05:57: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 11:05:57: #1 finished! INFO @ Sat, 15 Sep 2018 11:05:57: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 11:05:57: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 11:05:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:05:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:05:58: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 11:05:58: #2 number of paired peaks: 0 WARNING @ Sat, 15 Sep 2018 11:05:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 11:05:58: Process for pairing-model is terminated! cat: SRX4233703.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX4233703.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233703.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233703.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233703.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX4233703.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233703.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX4233703.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。