Job ID = 2010914 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,175,455 reads read : 17,175,455 reads written : 17,175,455 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1103931.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:18 17175455 reads; of these: 17175455 (100.00%) were unpaired; of these: 530609 (3.09%) aligned 0 times 13255436 (77.18%) aligned exactly 1 time 3389410 (19.73%) aligned >1 times 96.91% overall alignment rate Time searching: 00:03:18 Overall time: 00:03:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8614690 / 16644846 = 0.5176 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:22:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:22:52: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:22:52: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:22:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:22:53: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:22:53: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:22:59: 1000000 INFO @ Sat, 06 Jul 2019 01:22:59: 1000000 INFO @ Sat, 06 Jul 2019 01:23:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:23:01: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:23:01: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:23:06: 2000000 INFO @ Sat, 06 Jul 2019 01:23:06: 2000000 INFO @ Sat, 06 Jul 2019 01:23:09: 1000000 INFO @ Sat, 06 Jul 2019 01:23:13: 3000000 INFO @ Sat, 06 Jul 2019 01:23:14: 3000000 INFO @ Sat, 06 Jul 2019 01:23:18: 2000000 INFO @ Sat, 06 Jul 2019 01:23:20: 4000000 INFO @ Sat, 06 Jul 2019 01:23:21: 4000000 INFO @ Sat, 06 Jul 2019 01:23:26: 3000000 INFO @ Sat, 06 Jul 2019 01:23:28: 5000000 INFO @ Sat, 06 Jul 2019 01:23:28: 5000000 INFO @ Sat, 06 Jul 2019 01:23:34: 4000000 INFO @ Sat, 06 Jul 2019 01:23:34: 6000000 INFO @ Sat, 06 Jul 2019 01:23:35: 6000000 INFO @ Sat, 06 Jul 2019 01:23:41: 7000000 INFO @ Sat, 06 Jul 2019 01:23:43: 5000000 INFO @ Sat, 06 Jul 2019 01:23:43: 7000000 INFO @ Sat, 06 Jul 2019 01:23:49: 8000000 INFO @ Sat, 06 Jul 2019 01:23:49: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:23:49: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:23:49: #1 total tags in treatment: 8030156 INFO @ Sat, 06 Jul 2019 01:23:49: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:23:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:23:49: #1 tags after filtering in treatment: 8030156 INFO @ Sat, 06 Jul 2019 01:23:49: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:23:49: #1 finished! INFO @ Sat, 06 Jul 2019 01:23:49: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:23:49: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:23:50: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:23:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:23:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:23:50: 6000000 INFO @ Sat, 06 Jul 2019 01:23:50: 8000000 INFO @ Sat, 06 Jul 2019 01:23:51: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:23:51: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:23:51: #1 total tags in treatment: 8030156 INFO @ Sat, 06 Jul 2019 01:23:51: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:23:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:23:51: #1 tags after filtering in treatment: 8030156 INFO @ Sat, 06 Jul 2019 01:23:51: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:23:51: #1 finished! INFO @ Sat, 06 Jul 2019 01:23:51: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:23:51: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:23:51: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:23:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:23:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:23:57: 7000000 INFO @ Sat, 06 Jul 2019 01:24:05: 8000000 INFO @ Sat, 06 Jul 2019 01:24:05: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:24:05: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:24:05: #1 total tags in treatment: 8030156 INFO @ Sat, 06 Jul 2019 01:24:05: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:24:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:24:05: #1 tags after filtering in treatment: 8030156 INFO @ Sat, 06 Jul 2019 01:24:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:24:05: #1 finished! INFO @ Sat, 06 Jul 2019 01:24:05: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:24:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:24:06: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:24:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:24:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423156/SRX423156.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。