Job ID = 2010913 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,211,789 reads read : 17,211,789 reads written : 17,211,789 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1103930.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:06 17211789 reads; of these: 17211789 (100.00%) were unpaired; of these: 242721 (1.41%) aligned 0 times 13460404 (78.20%) aligned exactly 1 time 3508664 (20.39%) aligned >1 times 98.59% overall alignment rate Time searching: 00:03:06 Overall time: 00:03:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8895048 / 16969068 = 0.5242 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:25:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:25:51: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:25:51: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:25:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:25:52: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:25:52: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:26:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:26:18: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:26:18: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:26:24: 1000000 INFO @ Sat, 06 Jul 2019 01:26:28: 1000000 INFO @ Sat, 06 Jul 2019 01:26:28: 1000000 INFO @ Sat, 06 Jul 2019 01:26:32: 2000000 INFO @ Sat, 06 Jul 2019 01:26:37: 2000000 INFO @ Sat, 06 Jul 2019 01:26:39: 2000000 INFO @ Sat, 06 Jul 2019 01:26:40: 3000000 INFO @ Sat, 06 Jul 2019 01:26:45: 3000000 INFO @ Sat, 06 Jul 2019 01:26:47: 4000000 INFO @ Sat, 06 Jul 2019 01:26:50: 3000000 INFO @ Sat, 06 Jul 2019 01:26:55: 4000000 INFO @ Sat, 06 Jul 2019 01:26:55: 5000000 INFO @ Sat, 06 Jul 2019 01:27:01: 4000000 INFO @ Sat, 06 Jul 2019 01:27:02: 6000000 INFO @ Sat, 06 Jul 2019 01:27:04: 5000000 INFO @ Sat, 06 Jul 2019 01:27:10: 7000000 INFO @ Sat, 06 Jul 2019 01:27:12: 5000000 INFO @ Sat, 06 Jul 2019 01:27:13: 6000000 INFO @ Sat, 06 Jul 2019 01:27:18: 8000000 INFO @ Sat, 06 Jul 2019 01:27:18: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:27:18: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:27:18: #1 total tags in treatment: 8074020 INFO @ Sat, 06 Jul 2019 01:27:18: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:27:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:27:18: #1 tags after filtering in treatment: 8074020 INFO @ Sat, 06 Jul 2019 01:27:18: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:27:18: #1 finished! INFO @ Sat, 06 Jul 2019 01:27:18: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:27:18: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:27:19: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:27:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:27:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:27:22: 7000000 INFO @ Sat, 06 Jul 2019 01:27:24: 6000000 INFO @ Sat, 06 Jul 2019 01:27:30: 8000000 INFO @ Sat, 06 Jul 2019 01:27:31: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:27:31: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:27:31: #1 total tags in treatment: 8074020 INFO @ Sat, 06 Jul 2019 01:27:31: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:27:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:27:31: #1 tags after filtering in treatment: 8074020 INFO @ Sat, 06 Jul 2019 01:27:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:27:31: #1 finished! INFO @ Sat, 06 Jul 2019 01:27:31: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:27:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:27:32: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:27:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:27:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:27:35: 7000000 INFO @ Sat, 06 Jul 2019 01:27:45: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 01:27:46: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 01:27:46: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 01:27:46: #1 total tags in treatment: 8074020 INFO @ Sat, 06 Jul 2019 01:27:46: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:27:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:27:46: #1 tags after filtering in treatment: 8074020 INFO @ Sat, 06 Jul 2019 01:27:46: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:27:46: #1 finished! INFO @ Sat, 06 Jul 2019 01:27:46: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:27:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:27:46: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:27:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:27:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX423155/SRX423155.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。